Group B streptococcus selective culture medium, identification culture medium and using method thereof

Abstract

The invention provides a group B streptococcus selective culture medium and a using method thereof, and belongs to the field of microbial culture media. 1 liter of the culture medium comprises the following components: 1 to 10 grams of heart extract, 1 to 50 grams of new peptone, 1 to 10 grams of dextroglucose, 1 to 10 grams of NaCl, 0.1 to 5 grams of Na2HPO4, 1 to 10 grams of Na2CO3, 1 to 50 milligrams of gentamycin, 1 to 50 milligrams of nalidixic acid and the balance of water. The invention also provides a using method for the culture medium. The invention has the advantages that: the group B streptococcus selective culture medium provided by the invention can inhibit the growth of other bacteria, and if a sample has group B streptococcus, the streptococcus can be normally grown to predominant bacteria so that the culture medium brings great convenience to the subsequent identification operation and improves the detection accuracy and the detection efficiency of the group B streptococcus; and the using method for the selective culture medium is simple in operation and does not need special instruments and equipment.

Description

technical field

[0001] The invention relates to a microorganism culture medium, in particular to a group B streptococcus selective culture medium. Background technique

[0002] Group B Streptococcus (GBS), also known as Streptococcus agalactiae, is a Gram-positive bacterium that inhabits the human gastrointestinal tract and lower reproductive tract. It is one of the important pathogens of infectious diseases and has attracted widespread attention all over the world. GBS infection is the main cause of perinatal intrauterine infection, neonatal pneumonia, sepsis, meningitis and other serious infections. It can lead to serious consequences such as premature birth, fetal growth retardation (low birth weight infant), premature rupture of membranes and late miscarriage, and occupies an important position in perinatal medicine.

[0003] Neonatal GBS infection is mainly caused by the vertical infection of the mother. It is reported that the rate of vaginal bacteria in the delivery...

Examples

Embodiment 1

[0044] According to the composition of Example 1 given in Table 1, dissolve the heart extract, new peptone, dextrose, sodium chloride, disodium hydrogen phosphate, and sodium carbonate in distilled water, and set the volume to 1 L, and sterilize at 121°C After 15-30 minutes, cool to room temperature, add gentamicin and nalidixic acid, mix well, distribute into sterile test tubes, and package for later use. The nalidixic acid is one of antibacterial synthetic compounds, has a broad antibacterial spectrum, and is particularly effective against Gram-negative bacteria, and its mechanism of action is to inhibit the synthesis of bacterial deoxynucleotides. Gentamicin is an aminoglycoside antibiotic that is effective against Escherichia coli, Bacillus aerogenes, Klebsiella, Proteus mirabilis, some indole-positive Proteus, Pseudomonas aeruginosa, some Neisseria, some Gram-negative bacteria such as Serratia pigment and Shigella have antibacterial effects, and also have antibacterial ef...

Embodiment 2

[0046] According to the composition of Example 2 given in Table 1, the heart extract, new peptone, dextrose, sodium chloride, disodium hydrogen phosphate, and sodium carbonate were dissolved in distilled water, and the volume was adjusted to 1 L, and extinguished at 121°C. Bacteria for 15-30min, cooled to room temperature, added gentamicin, nalidixic acid and fluconazole, mixed evenly, divided into sterile test tubes, packaged for later use. The fluconazole can highly selectively inhibit the cytochrome P-450 of fungi, so that the bacterial cells lose normal sterols, while the 14α-methyl sterols accumulate in the bacterial cells and play a bacteriostatic effect. Bacteria and other Candida, Aspergillus flavus, Aspergillus fumigatus, Blastomyces dermatitidis, Coccidioides immobilis, Histoplasma capsulata, etc. have antibacterial effects.

Embodiment 3

[0048] According to the composition of Example 3 given in Table 1, the heart extract, new peptone, dextrose, sodium chloride, disodium hydrogen phosphate, and sodium carbonate were dissolved in distilled water, and the volume was adjusted to 1 L, and extinguished at 121°C. Bacteria for 15-30min, cooled to room temperature, added gentamicin, nalidixic acid and fluconazole, mixed evenly, divided into sterile test tubes, packaged for later use.

[0049] Examples 4-6 in Table 1 can be prepared in a similar manner to the above, and will not be repeated here. The sterilization steps in the above embodiments can also be replaced by other conventional sterilization methods, which will not be repeated here. The sterile test tubes in the above examples can also be replaced by other conventional sterile containers.

[0050] 2. Preparation of transport medium in selective culture of group B streptococci.

technical field

[0001] The invention relates to a microorganism culture medium, in particular to a group B streptococcus selective culture medium. Background technique

[0002] Group B Streptococcus (GBS), also known as Streptococcus agalactiae, is a Gram-positive bacterium that inhabits the human gastrointestinal tract and lower reproductive tract. It is one of the important pathogens of infectious diseases and has attracted widespread attention all over the world. GBS infection is the main cause of perinatal intrauterine infection, neonatal pneumonia, sepsis, meningitis and other serious infections. It can lead to serious consequences such as premature birth, fetal growth retardation (low birth weight infant), premature rupture of membranes and late miscarriage, and occupies an important position in perinatal medicine.

[0003] Neonatal GBS infection is mainly caused by the vertical infection of the mother. It is reported that the rate of vaginal bacteria in the delivery...

Examples

Embodiment 1

[0044] According to the composition of Example 1 given in Table 1, dissolve the heart extract, new peptone, dextrose, sodium chloride, disodium hydrogen phosphate, and sodium carbonate in distilled water, and set the volume to 1 L, and sterilize at 121°C After 15-30 minutes, cool to room temperature, add gentamicin and nalidixic acid, mix well, distribute into sterile test tubes, and package for later use. The nalidixic acid is one of antibacterial synthetic compounds, has a broad antibacterial spectrum, and is particularly effective against Gram-negative bacteria, and its mechanism of action is to inhibit the synthesis of bacterial deoxynucleotides. Gentamicin is an aminoglycoside antibiotic that is effective against Escherichia coli, Bacillus aerogenes, Klebsiella, Proteus mirabilis, some indole-positive Proteus, Pseudomonas aeruginosa, some Neisseria, some Gram-negative bacteria such as Serratia pigment and Shigella have antibacterial effects, and also have antibacterial ef...

Embodiment 2

[0046] According to the composition of Example 2 given in Table 1, the heart extract, new peptone, dextrose, sodium chloride, disodium hydrogen phosphate, and sodium carbonate were dissolved in distilled water, and the volume was adjusted to 1 L, and extinguished at 121°C. Bacteria for 15-30min, cooled to room temperature, added gentamicin, nalidixic acid and fluconazole, mixed evenly, divided into sterile test tubes, packaged for later use. The fluconazole can highly selectively inhibit the cytochrome P-450 of fungi, so that the bacterial cells lose normal sterols, while the 14α-methyl sterols accumulate in the bacterial cells and play a bacteriostatic effect. Bacteria and other Candida, Aspergillus flavus, Aspergillus fumigatus, Blastomyces dermatitidis, Coccidioides immobilis, Histoplasma capsulata, etc. have antibacterial effects.

Embodiment 3

[0048] According to the composition of Example 3 given in Table 1, the heart extract, new peptone, dextrose, sodium chloride, disodium hydrogen phosphate, and sodium carbonate were dissolved in distilled water, and the volume was adjusted to 1 L, and extinguished at 121°C. Bacteria for 15-30min, cooled to room temperature, added gentamicin, nalidixic acid and fluconazole, mixed evenly, divided into sterile test tubes, packaged for later use.

[0049] Examples 4-6 in Table 1 can be prepared in a similar manner to the above, and will not be repeated here. The sterilization steps in the above embodiments can also be replaced by other conventional sterilization methods, which will not be repeated here. The sterile test tubes in the above examples can also be replaced by other conventional sterile containers.

[0050] 2. Preparation of transport medium in selective culture of group B streptococci.

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