53 results about "Premature rupture of membranes" patented technology

The invention relates to a combined assay kit for premature birth of a pregnant woman and fetal membrane premature rupture, which comprises a premature birth and fetal membrane premature rupture immunochromatographic assay test strip or comprises a premature birth immunochromatographic assay test strip and a fetal membrane premature rupture immunochromatographic assay test strip. More preferably, the combination mat and the nitrocellulose membrane of the premature birth immunochromatographic assay test strip are respectively provided with a premature birth assay immunolabelling antibody and a premature birth assay antibody; the combination mat and the nitrocellulose membrane of the fetal membrane premature rupture immunochromatographic assay test strip are respectively provided with a fetal membrane premature rupture assay immunolabelling antibody and a fetal membrane premature rupture assay antibody, or the immunolabelling antibodies and the antibodies are respectively coated onto the combination mats and the nitrocellulose membranes of the premature birth and fetal membrane premature rupture immunochromatographic assay test strip. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is capable of simultaneously diagnosing the premature birth and the fetal membrane premature rupture through primary sampling, is simple and convenient in application, and further, is capable of improving the accuracy of premature birth diagnosis. A powerful basis is provided for the next accurate treatment by combined assay results. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is suitable for popularization and application in a large scale.

The invention discloses a premature rupture of membrane (PROM) fast examination tool using an intercellular adhesion molecular (ICAM)-1 as an examination index. The tool comprises a gasket, an absorbent pad, a nitrocellulose membrane, a conjugate pad and a sample pad, wherein the absorbent pad, the nitrocellulose membrane, the conjugate pad and the sample pad are connected from top to bottom and adhered to the gasket; the conjugate pad is covered by the sample pad part; the nitrocellulose membrane is provided with a goat-anti-human ICAM-1 polyclonal antibody coated examination line and a goat-anti-mouse immunoglobulin G polyclonal antibody coated control line; the examination line is below and away from the control line at intervals; and the conjugate pad is a water absorbing fibber coated with conjugated mouse-anti-human ICAM-1 monoclonal antibody conjugate. A PROM fast examination kit using the ICAM-1 as the examination index consists of a kit body and the examination tool arranged in the kit body.

A method is taught for the accurate determination of the premature rupture of membranes (PROM), defined as spontaneous rupture of membranes before the onset of uterine contractions. More specifically, a lateral flow assay strip tests for at least two antigens to greatly limit or eliminate the possibility of false negatives. A built in timer in the cassette holding the lateral flow assay further increases the accuracy of the test. A collection buffer vial with self-contained shipping and dropper caps and built in stand is also taught.

The presence of 3-chlorotyrosine in vaginal secretions in a predictor of the likelihood preterm premature rupture of the fetal membranes, i.e., the chorioamnion and / or the occurrence of the risk of preterm labor. 3-chlorotyrosine is a marker for the excessive production of hypochlorous acid which causes focal areas of increased collagen destruction in the chorioamnion, preterm premature rupture of membranes, or increased production of postaglandin and preterm labor. The inventive method comprises determining the likelihood of the occurrence of preterm premature rupture of membranes or increased production of postaglandin and preterm labor in a pregnant female by obtaining a sample of the females vaginal secretions, and analyzing the secretions for the presence and amount of 3-chlorotyrosine in the sample. Various antibody-based tests can be used to measure 3-chlorotyrosine, and a number of neoantigens, useful in raising antibodies to 3-chlorotyrosine, are disclosed.

The invention discloses a premature rupture of membrane (PROM) detection kit using an intercellular adhesion molecular (ICAM)-1 as an examination index. The kit comprises a perforated plate coated with an ICAM-1 monoclonal antibody, biotin-labeled ICAM-1 monoclonal antibody (biotin-ICAM-1Ab) examination fluid, aidin-horseradish peroxidase combined with the biotin-labeled ICAM-1 monoclonal antibody, chromogenic substrate 3',3',5,5'-tetramethylbenzidine and ICAM-1 protein standard. A preparation method of the kit comprises the following steps of: (1) preparing the perforated plate coated with the ICAM-1 monoclonal antibody; (2) preparing the biotin-labeled ICAM-1 monoclonal antibody examination fluid; and (3) preparing the aidin-horseradish peroxidase, the chromogenic substrate 3',3',5,5'-tetramethylbenzidine and the ICAM-1 protein standard.

The invention discloses a test strip for fast detecting premature rupture of fetal membranes, which is formed in a way that a sample pad, a glass fiber membrane, a coating membrane and absorbent paper are sequentially overlapped and pasted on a substrate, wherein the glass fiber membrane is coated with a high-specificity monoclonal antibody of latex particle-labeled insulin-like growth factor binding protein-1 (IGFBP-1); and the coating membrane comprises a detection area coating an anti-IGFBP-1 specific monoclonal antibody and a control area coating an anti-mouse antibody. Compared with the radioimmunoassay method and the enzyme-linked immunosorbent assay (ELISA) method, the test strip disclosed by the invention has the advantages of operational safety, fast detection and the like, is simple and convenient and is applicable to individual detection. The test strip disclosed by the invention can be conveniently and fast operated by people without professional skills, can not cause damages and can realize timely on-site and household self detection of premature rupture of fetal membranes; and the result can be read easily.

The invention relates to a tool and a method for diagnosing premature rupture of a membrane by continuous monitoring, which are applicable to household. The tool consists of an upper isolation filter layer, a medium testing system and a lower absorption layer, wherein the testing system consists of an A-type solid-phase vector containing urease and pH indicator and a B-type solid-phase vector containing the pH indicator. The diagnosing method comprises the following steps that: when fluid from the lower body simultaneously soaks two solid-phase vectors, and color development on the A-type solid-phase vector and the B-type solid phase vector has differences, the fluid is urine; and when the color development and color gradation on the A-type solid-phase vector and the B-type solid-phase vector are completely same, the fluid is amniotic fluid. The tool and the method have the advantages of simple operation, higher sensitivity and specificity, continuous detection of discontinuous leakage of the amniotic fluid and real-time property.

The invention provides an air cushion used for lifting buttocks of a pregnant woman for treatment during premature rupture of the membrane of the pregnant woman. The air cushion comprises an upper part and a lower part, wherein the upper part is a longitudinal rectangle from an overlook view and is a triangle from top down from a lateral view, and an air inflation hole, an air inflation tube, an air inflation piston and an air inflation ball are arranged on a side surface; and the lower part is a transverse rectangle from the overlook view and is a rectangle slightly higher than the bottom edge of the triangle from the lateral view, and an air inflation hole, an air inflation tube, an air inflation piston and an air inflation ball are arranged on a side surface. The air cushion solves the problems that at present, when premature rupture of the membrane occurs on the pregnant woman, the amniotic fluid flows out, the buttocks are in urgent need of being lifted for treatment, while the traditional method which adopts toilet paper to support below the buttocks is unreliable in effect, inconvenient to use, unsafe and uncomfortable. The air cushion which is lifted gradually from top down can relieve the discomfort in the waist part, the back part and the shoulder part caused by lifting of the buttocks, can be folded, packaged and placed in a carried bag in a non-inflation state for emergency, and is portable. During premature rupture of the membrane of the pregnant woman, the pregnant woman lies the buttocks lifting air cushion, so that complications such as oligohydramnios, prolapse of umbilical cord, fetal distress, and even intrauterine death and the like caused by outflow of the amniotic fluid can be prevented or reduced, and the buttocks lifting operation for treatment is simple, convenient and safe.

Assay methods for identifying an increased risk of preterm delivery induced by premature rupture of the fetal membranes are provided. More specifically, the invention provides salivary protease assays indicative of amniochorion and fetal membrane concentrations of proteases involved in the degradation of the fetal membranes leading to rupture. An increase in salivary protease levels beyond normal or individual baseline provides an indication that protease levels in fetal membranes and / or the amniotic fluid are abnormally elevated. Abnormally elevated protease levels define an increased risk of a premature delivery caused by rupture of the fetal membranes. Thus, abnormally high levels of salivary protease levels indicates elevated risk of PROM, and provides an indication for therapeutic intervention to prevent PROM-induced preterm labor.

The invention discloses a detection kit for premature rupture of fetal membranes, which comprises a detection test paper strip or a test paper card for premature rupture of fetal membranes, a buffer and a cotton swab, the test paper strip or the test paper card comprise a detection zone (T) coated with anti-human insulin-like growth factor binding protein-1 monoclonal antibody 1 and a quality control zone (C) coated with anti-mice polyclonal antibody and colloidal gold coated with anti-human insulin-like growth factor binding protein-1 monoclonal antibody 2. The preparation method of the kit comprises the following steps: preparing a treating liquid, preparing the kit, preparing a colloidal gold compound, spraying metal, cutting, assembling, cutting, packaging and the like. The detection kit for premature rupture of fetal membranes has the advantages of good specificity, high sensitivity, rapid and simple detection, and no pain in a usage process, and can be used for self-test in household and test on bed.

The invention belongs to the field of medical health care technology, and particularly provides a screening method for difference protein of vaginal secretion in pregnant woman with premature ruptureof membranes based on iTRAQ technology. By combining the iTRAQ technology and the 2DLC-MS-MS technology, a rapid and high correlation degree comprehensively screening of the vaginal secretion difference protein of the premature woman with premature rupture of membranes is achieved, after further identification analysis, the screened differential proteins IGFBP-1, AFP, PRL and LZM can be used as biomarkers for diagnosis of premature rupture of membranes with higher sensitivity and specificity.

The invention discloses a posture pad for premature rupture of fetal membranes. The posture pad comprises a latex pad conforming to the human body structure and a waterproof membrane, wherein the waterproof membrane is disposed on the upper end face of the latex pad and matched with the latex pad for use; multiple through holes are formed in the middle of the latex pad, multiple liquid discharge pipes corresponding to the through holes one to one are arranged on the lower end face of the waterproof membrane and inserted into and penetrate through the through holes respectively; a supporting plate is provided on the lower end face of the latex pad, a through groove corresponding to the through holes is formed in the middle of the supporting plate, and a liquid collecting bracket is arrangedon the portion, located at the through groove, of the bottom of the supporting plate; a liquid collecting box is arranged in the liquid collecting bracket, a first connecting rod is rotatably connected to the lower plane of the supporting plate, and a supporting rod is disposed at the end, away from the supporting plate, of the first connecting rod; the middle of the first connecting rod is rotatably connected to a transmission block through a second connecting rod, the transmission block is disposed on a screw rod, the end of the screw rod is provided with a handle capable of driving the screw rod to rotate, and ascending and descending of the latex pad can be achieved by rotating the screw rod. The posture pad for premature rupture of the fetal membranes is not only simple in structurebut also low in processing cost, safe and hygienic.

The invention discloses application of a regent for detecting Axl receptor tyrosine kinase to prepare a kit for detecting whether a pregnant woman has a premature rupture of membranes. The regent is a monoclonal antibody of the Axl receptor tyrosine kinase which is labeled by biotin. The kit includes a perforated plate coated with the monoclonal antibody of the Axl receptor tyrosine kinase, biotin-labeled monoclonal antibody detection liquid of the Axl receptor tyrosine kinase, avidin-horse radish peroxidase combined with biotin-labeled monoclonal antibody of the Axl receptor tyrosine kinase, a chromogenic substrate 3',3',5,5'-tetramethylbenzidine, and an Axl protein standard substance. A preparation method for the kit comprises the following steps of: (1) preparing the perforated plate coated with the monoclonal antibody of the Axl receptor tyrosine kinase; (2) preparing the biotin-labeled monoclonal antibody detection liquid of the Axl receptor tyrosine kinase; and (3) preparing avidin-horse radish peroxidase, the chromogenic substrate 3',3',5,5'-tetramethylbenzidine, and the protein standard substance of the Axl receptor tyrosine kinase.

The invention provides a monoclonal antibody of human insulin-like growth factor binding protein-1, a monoclonal antibody host cell, a diagnostic reagent with the monoclonal antibody of the human insulin-like growth factor binding protein-1and a diagnostic reagent kit, wherein antigen of the monoclonal antibody is antigenic determinant of the human insulin-like growth factor binding protein-1; preferably, the antigenic determinant comprises an amino acid sequence represented by SEQIDNO:1; and the monoclonal antibody host cell contains the monoclonal antibody of the human insulin-like growth factor binding protein-1. The monoclonal antibody of the human insulin-like growth factor binding protein-1 can perform specific detection on the human insulin-like growth factor binding protein-1, and the prepared diagnostic reagent has high specificity of premature rupture of membrane diagnosis, rapid reaction, low cost and suitability for large-scale popularization and application.

This invention is about a method for the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic reagent system and diagnostic kit for the diagnosis. The method, diagnostic reagent system, and kit are based on the finding that the level of MMP-8 in the amniotic fluid is significantly higher when the pregnant woman is at risk for preterm delivery, intrauterine infection, and fetal damage. The diagnostic reagent system and kit can be applied to patients with or without clinical signs of preterm labor or premature rupture of fetal membranes. With its superiority in sensitivity and specificity as well as its less invasiveness compared to the conventional method of measuring fetal blood cytokine levels, this diagnostic reagent system and kit is very useful in the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage.

A method to identify women who are at risk for preterm delivery due to premature rupture of membranes (PPROM) is provided. The method entails detecting the presence of SERPINH1 gene variants that express low levels of the gene product, heat shock protein Hsp47. The occurrence of a T (rather than C) at a single nucleotide polymorphism (SNP) site at position −656 of the SERPINH1 gene promoter, together with the absence of a 12 base pair deletion at positions −694 to −683 of the promoter, result in an increased risk of PPROM. The method enables medical professionals to identify those at risk, and to provide suitable therapeutic intervention.

The present invention relates to the technical field of obstetrics and gynecology medical science, and particularly to a method for predicting the success rate of scarred uterus trial-production. The method comprises: collecting data of a pregnant woman, including antepartum parity, an antepartum BMI (Body Mass Index), a cervix prenatal situation, and whether PROM (premature rupture of membranes) occurs or not; performing calculation by using P = 1 / [1+exp(-0.785+1.3259x1-1.5061x2-1.2449x31-1.4211x32+0.7617x4)]*100%; and predicting the success rate of the pregnant woman uterus trial-production according to a P value, so as to provide a reference index for the pregnant woman and doctors. The method is easy in data collection, high in prediction success rate, and especially suitable for vagina trial-production prediction of Chinese pregnant women with a scarred uterus.

The invention discloses a detection kit and a preparation method thereof, specifically elates to a kit for detecting premature rupture of membrane and a preparation method thereof. The kit comprises a multi-cell plate and reagents, wherein the reagents comprise: A, a magnetic microsphere suspension, wherein the magnetic microspheres are prepared by co-cross-linking hydroxylation magnetic microspheres with a diameter of 0.5-2 mum, N-hydroxysulfo succinic acid amide and carbodiimide, and a concentration of the magnetic microspheres in the suspension is 75-85 / muL; B, phycoerythrin-labeled streptavidin and a biotin-labeled antibody detection solution, wherein the biotin is N-hydroxy succinic acid imino ester, the antibody is mouse anti-human soluble intercellular adhesion molecule-1 monoclonal antibody, and a concentration of the biotin-labeled antibody is 1.7-2 mg / mL; and C, a soluble intercellular adhesion molecule-1 protein standard substance and a standard substance dilution solution. The kit provides significantly increased sensitivity and specificity for the detection result of premature rupture of membrane so as to reduce diagnostic error incidence rate, wherein the sensitivity and the specificity can respectively achieve more than 99% and 98%.

The present invention provides compositions and methods for use in treatment of premature rupture of membranes. The compositions of the invention include a therapeutic amount of an avian thick egg white composition. Preferably, the compositions include the purified thick egg white protein, ovomucin. The methods of the invention involve injecting an effective amount of the compositions into the prematurely ruptured amniotic sac of a patient in order to seal the rupture.

The invention discloses a body position adjusting device for a pregnant woman with premature rupture of fetal membranes, and effectively solves the problem of excessive amniotic fluid loss during transfer and body position adjustment of the pregnant woman with premature rupture of fetal membranes. The body position adjusting device comprises a bed plate; a back plate is hinged to the right side ofthe bed plate through a supporting shaft; a hip plate is rotationally connected to the supporting shaft; a rotating shaft is rotationally connected to the left side of the hip plate; a connecting plate is fixed to the rotating shaft; leg plates are rotationally connected to the front side and the rear side of the connecting plate; supporting plates are arranged on the front side and the rear sideof the bed plate; arc-shaped grooves are formed in the supporting plates; kidney-shaped grooves communicating with the upper side of the arc-shaped grooves are formed in the supporting plates; guidecolumns are arranged on the opposite sides of the two leg plates; the free ends of the guide columns are inserted into the corresponding kidney-shaped grooves; a first rack is slidably connected to the interior of the bed plate; a connecting rod is hinged to the right end of the first rack; the upper end of the connecting rod is hinged to the back plate; chutes are formed in the front and rear sides of the bed board; insertion columns are arranged at the lower ends of the leg plates; and the lower ends of the insertion columns are inserted in the corresponding chutes. The device is novel in concept, ingenious in structure, convenient to operate and high in practicability.

A method is taught for the accurate determination of the premature rupture of membranes (PROM), defined as spontaneous rupture of membranes before the onset of uterine contractions. More specifically, a lateral flow assay strip tests for at least two antigens to greatly limit or eliminate the possibility of false negatives. A built in timer in the cassette holding the lateral flow assay further increases the accuracy of the test. A collection buffer vial with self-contained shipping and dropper caps and built in stand is also taught.

The invention discloses a premature rupture of membranes colloidal gold test paper reaction buffer solution and a preparation method thereof. 1.2 to 7.8 parts of trisodium citrate are dissolved in 100 parts of purified water to form solution A; 0.1 to 1.2 parts of citric acid are dissolved in 100 parts of Parts of purified water to make solution B; use solution B to adjust solution A to pH 6.50-10.00 to make sodium citrate buffer solution; 100 parts of sodium citrate buffer solution, 1-5 parts of BSA, 0.5-0.9 parts of Nacl, Add 0.2 to 2 parts of TritonX1000 and 0.01 to 0.06 parts of Proclin3000 into sodium citrate buffer, stir evenly, and make reaction buffer for premature rupture of membranes colloidal gold test paper. The invention can greatly increase the migration speed of the colloidal gold test paper, shorten the appearance time of the detection line and the quality control line, and has common components of the buffer solution, is relatively cheap in price, and the whole preparation is also relatively convenient, and the preparation process is simple and easy to operate.

The invention discloses a biomarker and a method for predicting premature rupture of fetal membranes, and the biomarker is selected from one or more of 4-methylvaleraldehyde (isohexanal), sphingolipids (SP) metabolite, glycerolipids (GL) metabolite and glycerophospholipids (GP) metabolite; the biomarker has a change in content difference multiple between a pregnant female body and a healthy pregnant female body at risk of premature rupture of fetal membranes. The biomarker for predicting premature rupture of fetal membranes and the prediction method have important significance for early prediction of premature rupture of fetal membranes before delivery and formulation of corresponding intervention measures.

The invention relates to an aptamer capable of being specifically combined with ICAM-1. The aptamer has the high combining characteristic and the high stability. The aptamer is obtained in the mode that the new combinatorial chemistry technology SELEX is applied, ICAM-1 serves as a target, and the DNA aptamer capable of being specifically combined with the ICAM-1 is screened out from a random library. The invention further relates to a kit for identifying the premature rupture of fetal membranes, and the kit can rapidly identify the premature rupture of fetal membranes, and has quite high application value.

The invention discloses a preparation method of an immunochromatographic test strip, and specifically relates to a preparation method of an immunochromatographic test strip for detecting premature rupture of membrane. the preparation of an immunochromatographic membrane in the preparation method is characterized in that a goat anti-human soluble intercellular adhesion molecule-1 polyclonal antibody is taken as a working solution of a detection line, and a goat anti-mouse IgG polyclonal antibody is taken as a working solution of a mass control line; the concentration of the working solution of the detection line is 1.5-2.5 mg / mL, and the concentration of the working solution of the mass control line is 0.5-1.5 mg / mL; and a nitrocellulose membrane is coated with the working solution of the detection line and the working solution of the mass control line, and then is dried at 36-38 DEG C for 8-12 hours to get the immunochromatographic membrane. The sensitivity and the specificity of the immunochromatographic test strip for detecting the premature rupture of membrane are improved obviously to be more than 97% and more than 93% respectively, and thus the occurrence rate of error diagnosis is reduced.

A method is taught for the accurate determination of the premature rupture of membranes (PROM), defined as spontaneous rupture of membranes before the onset of uterine contractions. More specifically, a lateral flow assay strip tests for at least two antigens to greatly limit or eliminate the possibility of false negatives. A built-in timer in the cassette holding the lateral flow assay further increases the accuracy of the test. A collection buffer vial with self-contained shipping and dropper caps and built-in stand is also taught.