Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture

A technology for premature rupture of membranes and a kit, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of increasing premature fetal membranes, premature rupture, uterine contractions or infection in pregnant women, and achieves improved accuracy and easy application. Effect

Inactive Publication Date: 2012-05-09
WUXI CITY KAIAOSHAN BIOPHARML TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the above-mentioned clinical diagnoses of premature labor and premature rupture of membranes are carried out separately; this kind of treatment increases the complexity of the operation, and at the same time, because of the need to frequently sample pregnant women, it will stimulate pregnant women to produce uterine contractions or infection chance, which in turn increases the risk of preterm birth and premature rupture of membranes

Method used

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  • Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture
  • Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture
  • Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Colloidal gold labeling

[0042] Antibody (anti-human phosphorylated insulin-like growth factor binding protein-1) colloidal gold labeling method for premature birth diagnosis is as follows: adjust the pH value of 50ml colloidal gold solution to 7.7 with potassium carbonate solution; Add 0.65ml 1mg / ml anti-human phosphorylated insulin-like growth factor binding protein-1 antibody to the above colloidal gold solution, mix well, and let it stand at room temperature for 30min; slowly add 12.5ml blocking solution to the above colloidal gold solution, mix well , stand at room temperature for 30min; centrifuge at 8000g 4°C for 1h, remove the supernatant, and leave the loose precipitate; dissolve the precipitate in each tube with 25ml washing solution, centrifuge at 6000g 4°C for 1h, remove the supernatant, leave the loose precipitate; wash the precipitate in each tube with 1.2ml solution, transferred to a 1.5ml centrifuge tube, centrifuged at 4500g for 1 hour at 4°C...

Embodiment 2

[0044] Spray point of embodiment 2 colloidal gold pad

[0045] The method of spraying the colloidal gold pad of the antibody (anti-human phosphorylated insulin-like growth factor binding protein-1) for premature birth diagnosis is as follows: colloidal gold antibody solution dilution: the colloidal gold antibody prepared above is combined with the colloidal gold antibody resuspension Dilute the substance by 4 times; set the dot film device, turn on the power of the dot film device, set the spray point program, the spray point volume is 8ul / cm; the No. 1 pipeline is the spray point channel; the dot film device initialization: set the No. In the colloidal gold antibody resuspension solution, select the initialization program and initialize 6 cycles; spray spot: place the gold label pad flat on the film spotting instrument at a fixed position, press the "GO" button on the control panel to start spraying, and finish spotting Then take it off and check the sprayed gold label pad. T...

Embodiment 3

[0047] The preparation of embodiment 3 nitrocellulose membranes

[0048] The antibody (anti-human phosphorylated insulin-like growth factor binding protein-1) nitrocellulose membrane preparation method for preterm birth diagnosis is as follows: take 500ug of goat anti-human IGFBP-1 polyclonal antibody, add it to a 5ml graduated centrifuge tube, and dilute the antibody Liquid to 1ml, the container is marked with a T mark. Take 25ul of goat anti-mouse IgG antibody, add it to a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container with a C mark. Set the film spotting instrument, turn on the power supply of the film spotting instrument, set the spray point program, and the spray point volume is 1ul / cm; the No. 1 pipeline is the detection channel with spray points, and the No. 2 pipeline is the control channel with spray point points; the film spotting instrument Initialization: put the No. 1 pipe in the detection zone solution, put the No. 2 pipe in th...

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Abstract

The invention relates to a combined assay kit for premature birth of a pregnant woman and fetal membrane premature rupture, which comprises a premature birth and fetal membrane premature rupture immunochromatographic assay test strip or comprises a premature birth immunochromatographic assay test strip and a fetal membrane premature rupture immunochromatographic assay test strip. More preferably, the combination mat and the nitrocellulose membrane of the premature birth immunochromatographic assay test strip are respectively provided with a premature birth assay immunolabelling antibody and a premature birth assay antibody; the combination mat and the nitrocellulose membrane of the fetal membrane premature rupture immunochromatographic assay test strip are respectively provided with a fetal membrane premature rupture assay immunolabelling antibody and a fetal membrane premature rupture assay antibody, or the immunolabelling antibodies and the antibodies are respectively coated onto the combination mats and the nitrocellulose membranes of the premature birth and fetal membrane premature rupture immunochromatographic assay test strip. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is capable of simultaneously diagnosing the premature birth and the fetal membrane premature rupture through primary sampling, is simple and convenient in application, and further, is capable of improving the accuracy of premature birth diagnosis. A powerful basis is provided for the next accurate treatment by combined assay results. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is suitable for popularization and application in a large scale.

Description

technical field [0001] The invention relates to the technical field of detection kits, in particular to the technical field of joint detection kits, in particular to a joint detection kit for premature birth and premature rupture of membranes in pregnant women. Background technique [0002] Birth between 28 weeks and less than 37 weeks of gestation is called premature birth. Preterm birth is divided into two types: spontaneous premature birth and therapeutic premature birth. Spontaneous premature birth includes preterm delivery and preterm premature rupture of membranes. Therapeutic premature birth is a pregnancy complication or complication that requires early termination of pregnancy; among them, spontaneous Preterm births account for the vast majority. [0003] At present, there are mainly the following methods for clinical diagnosis of premature birth: [0004] 1) Cervical assessment: measure the length and structural changes of the cervix. Generally, when the length o...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 雷震韦彦余
Owner WUXI CITY KAIAOSHAN BIOPHARML TECH
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