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Screening method for difference protein of vaginal secretion in pregnant woman with premature rupture of membranes based on iTRAQ technology

A technology for vaginal secretions and differential proteins, which is applied in the field of healthcare, can solve the problems of good specificity and inability to obtain sensitivity, and achieve the effects of accurate screening, fast screening, and comprehensive results

Inactive Publication Date: 2019-01-04
重庆早柒天生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the currently disclosed methods for screening biomarkers of premature rupture of membranes have some defects, resulting in the inability to obtain biomarkers with high sensitivity and good specificity

Method used

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  • Screening method for difference protein of vaginal secretion in pregnant woman with premature rupture of membranes based on iTRAQ technology
  • Screening method for difference protein of vaginal secretion in pregnant woman with premature rupture of membranes based on iTRAQ technology
  • Screening method for difference protein of vaginal secretion in pregnant woman with premature rupture of membranes based on iTRAQ technology

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Embodiment

[0034] This embodiment provides a screening method for differential proteins in vaginal secretions of pregnant women with premature rupture of membranes based on iTRAQ technology. In this embodiment, the differential proteins obtained through screening are further identified and verified to determine the screened differential proteins. Feasibility of differential proteins as biomarkers for premature rupture of membranes. The specific scheme content of this embodiment is as follows:

[0035] 1. Materials and methods

[0036] 1. Clinical data

[0037] The specimens came from 60 patients who visited Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University from March 2017 to June 2017. The research content and research protocol of this example were approved by the Ethics Committee of Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University. Those who met the following conditions were enrolled: 1) pregnant women who were pregn...

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Abstract

The invention belongs to the field of medical health care technology, and particularly provides a screening method for difference protein of vaginal secretion in pregnant woman with premature ruptureof membranes based on iTRAQ technology. By combining the iTRAQ technology and the 2DLC-MS-MS technology, a rapid and high correlation degree comprehensively screening of the vaginal secretion difference protein of the premature woman with premature rupture of membranes is achieved, after further identification analysis, the screened differential proteins IGFBP-1, AFP, PRL and LZM can be used as biomarkers for diagnosis of premature rupture of membranes with higher sensitivity and specificity.

Description

technical field [0001] The invention belongs to the technical field of medical care, and in particular relates to a method for screening differential proteins in vaginal secretions of pregnant women with premature rupture of membranes based on iTRAQ technology. Based on the screening method, the present invention also provides the differential protein in vaginal secretions of pregnant women with premature rupture of membranes obtained by the screening method, and further provides the application of the differential protein as a biomarker in the preparation of products for diagnosing fetal membranes. Background technique [0002] Premature rupture of membranes refers to the rupture of membranes before labor, which is a common obstetric complication with an incidence rate of 2.7%-7% [Xie Xing, Gou Wenli. Obstetrics and Gynecology. 8th Edition [M]. People's Health Publishing House, 2013 :133-135.]. Preterm rupture of membranes can cause serious adverse consequences to pregnant...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 王建东
Owner 重庆早柒天生物科技股份有限公司
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