Detection kit for premature rupture of fetal membranes and preparation method thereof
A detection kit and a technology for premature rupture of membranes, applied in biological testing, material inspection products, etc., can solve the problems of high requirements for operators, need to operate instruments, and low sensitivity, and achieve high sensitivity, simple operation, and specificity Good results
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[0026] The preparation method of the premature rupture of membranes detection kit includes two major steps: the configuration of the treatment solution and the preparation of the kit, and the specific steps are as follows:
[0027] 1) Preparation of treatment solution
[0028] Preparation of A gold standard pad treatment solution: Tris-Hcl (10mM, PH=7.4)+0.5%PVP+0.5%TW-20
[0029] Preparation of B sample pad treatment solution: Tris-Hcl (0.1MPH=9.0)+1%PVP+1.0%S17+0.8%Casein+0.3%TritonX-100
[0030] C spot membrane antibody dilution preparation: PBS {PB (10mM, PH = 7.4), 0.85% NaCl}
[0031] Preparation of D-labeled antibody diluent: PB (10mM, PH=7.4)
[0032] Preparation of E colloidal gold complex dilution: PB (10mM, PH=7.4)+20% sucrose+1%BSA
[0033] 2) Preparation of the kit
[0034] A treatment of membrane materials:
[0035] a. Treatment of sample pad: The sample pad (SB08) used by generals is soaked with the prepared treatment solution. After fully soaking, it can b...
Embodiment
[0058] By adopting the aforementioned method of use, the verification test of 200 routine clinical samples has been carried out to the premature rupture of membranes detection kit (insulin-like growth factor binding protein-1 detection kit) (colloidal gold immunochromatography) of the present invention, and the control group adopts traditional The standard for the diagnosis of premature rupture of membranes is to use pH test paper to detect the pH of the vaginal secretions > 7 or to see fern-shaped crystals in the vaginal secretions directly under the microscope and to see a small amount of liquid flowing out of the cervix in vaginal speculum examination. The results showed that there was no significant difference between the test product and the control group, and the test product reached the gold standard result of clinical diagnosis.
[0059]
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