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A kind of premature rupture of membranes colloidal gold test paper reaction buffer and preparation method thereof

A technology of colloidal gold test paper and reaction buffer, which is applied in the field of medical testing, can solve problems such as insufficient migration speed and slow detection line speed, and achieve the effects of convenient preparation, improved migration speed, and low price

Inactive Publication Date: 2011-12-28
吉权 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the buffer solution used for the reaction of colloidal gold test paper for premature rupture of membranes does not migrate fast enough and the quality control line and detection line appear slowly

Method used

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  • A kind of premature rupture of membranes colloidal gold test paper reaction buffer and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Weigh 1.2 g of trisodium citrate and dissolve in 100 ml of purified water to make solution A; weigh 0.1 g of citric acid and dissolve in 100 ml of purified water to make solution B; use solution B to adjust solution A to pH6. 50, make solution C

[0020] (2) Measure 100 ml of solution C, weigh 1 g of BSA, 0.5 g of Nacl, 0.2 g of TritonX100, and 0.01 ml of Proclin300 into solution C, stir evenly, and make reaction buffer for premature rupture of membranes colloidal gold test paper;

Embodiment 2

[0022] (1) Weigh 1.5 g of trisodium citrate and dissolve in 100 ml of purified water to make solution A; weigh 0.2 g of citric acid and dissolve in 100 ml of purified water to make solution B; use solution B to adjust solution A to pH7. 00, made into solution C

[0023] (2) Measure 100 ml of solution C, weigh 1.5 g of BSA, 0.6 g of Nacl, 0.3 g of TritonX100, and 0.02 ml of Proclin300 into solution C, stir evenly, and prepare the reaction buffer for premature rupture of membranes colloidal gold test paper;

Embodiment 3

[0025] (1) Weigh 2.0 g of trisodium citrate and dissolve in 100 ml of purified water to make solution A; weigh 0.4 g of citric acid and dissolve in 100 ml of purified water to make solution B; use solution B to adjust solution A to pH7. 50, make solution C

[0026] (2) Measure 100 ml of solution C, weigh 2.0 g of BSA, 0.8 g of Nacl, 0.5 g of TritonX100, and 0.03 ml of Proclin300 into solution C, stir well, and make reaction buffer for premature rupture of membranes colloidal gold test paper;

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Abstract

The invention discloses a premature rupture of membranes colloidal gold test paper reaction buffer solution and a preparation method thereof. 1.2 to 7.8 parts of trisodium citrate are dissolved in 100 parts of purified water to form solution A; 0.1 to 1.2 parts of citric acid are dissolved in 100 parts of Parts of purified water to make solution B; use solution B to adjust solution A to pH 6.50-10.00 to make sodium citrate buffer solution; 100 parts of sodium citrate buffer solution, 1-5 parts of BSA, 0.5-0.9 parts of Nacl, Add 0.2 to 2 parts of TritonX1000 and 0.01 to 0.06 parts of Proclin3000 into sodium citrate buffer, stir evenly, and make reaction buffer for premature rupture of membranes colloidal gold test paper. The invention can greatly increase the migration speed of the colloidal gold test paper, shorten the appearance time of the detection line and the quality control line, and has common components of the buffer solution, is relatively cheap in price, and the whole preparation is also relatively convenient, and the preparation process is simple and easy to operate.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a reaction buffer solution of an in vitro diagnostic reagent for medical use. Background technique [0002] Colloidal gold test strips belong to the category of immunological detection, and the method is specific and sensitive. In 1974, Romano et al. labeled colloidal gold on the secondary antibody (horse anti-human IgG), and established an indirect immunocolloidal gold staining method. In 1978, geoghega discovered the application of colloidal gold markers at the level of light microscopy. The application of colloidal gold in immunochemistry is also called immunogold. Afterwards, many scholars further confirmed that colloidal gold can adsorb proteins stably and rapidly, while the biological activity of proteins has no obvious change. It can be used as a probe for precise positioning of polysaccharides, proteins, polypeptides, antigens, hormones, nucleic acids and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/532G01N33/68
Inventor 吉权李民友潘能科
Owner 吉权
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