Test strip for fast detecting premature rupture of fetal membranes

A technology for detecting test strips and premature rupture of membranes, applied in the field of medical testing, can solve the problems of inability to judge the time and stage of rupture of fetal membranes, difficult to achieve quantitative detection, complicated purification process, etc., to achieve easy-to-read and optimized results. Preparation method and effect of improving detection sensitivity

Inactive Publication Date: 2011-05-11
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] ① Nitrate (pH) test paper: According to the alkaline nature of the amniotic fluid, the Nitrate test paper will turn blue, so as to achieve the purpose of detection. Negative Mirror Test Sampling
The disadvantage of the above-mentioned prior art is that the use of colloidal gold to mark markers requires contact with heavy metals during the preparation process, and the separation and purification process of colloidal gold is complicated, and it is difficult to achieve quantitative detection, and it is impossible to judge the time and stage of fetal membrane rupture

Method used

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  • Test strip for fast detecting premature rupture of fetal membranes
  • Test strip for fast detecting premature rupture of fetal membranes
  • Test strip for fast detecting premature rupture of fetal membranes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Premature rupture of membranes color latex rapid detection test strip

[0033] like figure 1 As shown, the rapid detection test strip for premature rupture of membranes according to the present invention, the test strip is that the sample pad 1 is pasted on the backing 7 in sequence, and the colored latex particles are coated with the IGFBP-1 monoclonal antibody. Made of glass fiber membrane 2, coating membrane 3 and absorbent paper 4, the coating membrane 3 has a detection area 5 and a control area 6, the detection area 5 is coated with anti-IGFBP-1 monoclonal antibody, and the control area 6 is coated with anti-mouse antibody.

[0034] The ratio of IGFBP-1 monoclonal antibody to latex particles is 10 μg / 100 μl, and the dilution parameter of latex particle-labeled IGFBP-1 monoclonal antibody coated on glass fiber membrane is 25 cm 2 / ml. The concentration of anti-IGFBP-1 specific monoclonal antibody and anti-mouse antibody was 10 μg / ml, and the amount used ...

Embodiment 2

[0051] Example 2 Premature Rupture of Membranes Magnetic Latex Rapid Detection Test Strip

[0052] The detection test strip in this embodiment is the same as the embodiment 1 except that the glass fiber membrane 2 is coated with the magnetic latex-labeled IGFBP-1 monoclonal antibody. The magnetic latex particles are core-shell silica magnetic microspheres, the surface group is carboxyl (-COOH), solid content: 10%, particle size: 500nm (deviation<10%), and magnetic content is 20%.

[0053] The ratio of IGFBP-1 monoclonal antibody to magnetic latex particles is 100 μg / 100 μl, and the dilution parameter of latex particle-labeled IGFBP-1 monoclonal antibody coated on glass fiber membrane is 30 cm 2 / ml. The concentration of anti-IGFBP-1 specific monoclonal antibody and anti-mouse antibody was 10 μg / ml, and the amount used in the detection zone and control zone was 20 μl / 35cm.

[0054] The preparation method of the detection test strip in this embodiment is:

[0055] 1. Preparat...

Embodiment 3

[0065] Example 3 Fluorescent latex rapid detection test strip for premature rupture of membranes

[0066] In the detection test strip in this embodiment, the glass fiber membrane 2 is coated with fluorescent latex-labeled IGFBP-1 monoclonal antibody, and the other structures are the same as in Embodiment 1. The fluorescent latex particles are coupled with the IGFBP-1 antibody, and the quantitative detection of IGFBP-1 is realized through the relationship curve of the antibody coupling amount, the concentration of the antibody drawn on the detection line and the detection signal.

[0067] Among them, the fluorescent latex particles are fluorescent polymer microspheres formed by combining fluorescein and a polymer with styrene as a monomer, with an average particle size of 200-250nm and a maximum excitation wavelength of 550nm.

[0068] The ratio of IGFBP-1 monoclonal antibody to fluorescent latex particles is 50 μg / 100 μl, and the dilution parameter of fluorescent latex particl...

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Abstract

The invention discloses a test strip for fast detecting premature rupture of fetal membranes, which is formed in a way that a sample pad, a glass fiber membrane, a coating membrane and absorbent paper are sequentially overlapped and pasted on a substrate, wherein the glass fiber membrane is coated with a high-specificity monoclonal antibody of latex particle-labeled insulin-like growth factor binding protein-1 (IGFBP-1); and the coating membrane comprises a detection area coating an anti-IGFBP-1 specific monoclonal antibody and a control area coating an anti-mouse antibody. Compared with the radioimmunoassay method and the enzyme-linked immunosorbent assay (ELISA) method, the test strip disclosed by the invention has the advantages of operational safety, fast detection and the like, is simple and convenient and is applicable to individual detection. The test strip disclosed by the invention can be conveniently and fast operated by people without professional skills, can not cause damages and can realize timely on-site and household self detection of premature rupture of fetal membranes; and the result can be read easily.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a rapid detection and diagnosis test strip for premature rupture of membranes. Background technique [0002] Premature rupture of membranes (premature rupture of membranes, PROM) is the spontaneous rupture of fetal membranes before labor, gestational age <37 weeks of gestational premature rupture of membranes is also known as premature (premature rupture of membranes, preterm Premature rupture of membranes, PPROM). Premature rupture of membranes is the most common perinatal complication and can have serious adverse consequences for the mother, fetus, and newborn. Premature rupture of membranes can lead to increased rates of premature birth, increased perinatal mortality, and increased rates of intrauterine infection and puerperal infection. The causes of premature rupture of membranes include: trauma, relaxation of the internal os of the cervix, ascending infection of pathogeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 王继华
Owner GUANGZHOU WONDFO BIOTECH
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