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Nocardia asteroid selective medium for sputum samples

A Nocardia stellatus, selective culture medium technology, applied in the field of Nocardia stellatus selective medium for sputum specimens, can solve the problems of low positive rate, death, focus transfer, etc., and achieve reliable detection and separation high rate effect

Inactive Publication Date: 2015-08-12
王均梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nocardia stellatus grows slowly, and pathogenic bacteria such as Staphylococcus aureus, Streptococcus pneumoniae, and other actinomycetes in sputum specimens grow faster than Nocardia stellate on the current medium, and soon Covering the culture medium, Nocardia asteroides cannot grow, and the positive rate is low. It takes 96 hours to show a positive expression using the traditional method, and it takes about 1 week to make a more accurate identification. The results are delayed and cause multiple lesions to metastasize , if transferred to the brain, it can easily cause death

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, the formula for preparing 1000ml medium contains: beef liver powder 15.0g; malt extract powder 2.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; porphyrin iron 0.01g; g; nalidixic acid 0.05g; chrysotrione 0.001g; vitamin K 1 0.1g; sterile defibrated sheep blood 100ml; add distilled water to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; Iron; Sterilized Nalidixic Acid; Sterilized Vitamin K 1 ; Mix the aseptic defibrated sheep blood evenly, dilute it with sterilized distilled water to 1000ml, and dispense it.

Embodiment 2

[0024] Example 2, the formula for preparing 1000ml medium contains: bovine liver powder 10.0g; malt extract powder 5.0g; yeast extract powder 8.0g; sodium chloride 5.0g; agar 15.0g; porphyrin iron 0.01g; g; nalidixic acid 0.03g; chrysotrione 0.003g; vitamin K 1 0.01g; complexin phosphopeptide 0.1g; sterile defibrinated sheep blood 80ml; add distilled water to 1000ml. Preparation method Weigh beef liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; calcium lactate; Ferromorph; Sterilized Nalidixic Acid; Sterilized Vitamin K 1 ; Sterilized Collin Phosphopeptide; Sterile defibrinated sheep blood mixed evenly, sterilized distilled water to 1000ml before packing.

Embodiment 3

[0025] Example 3, the formula for preparing 1000ml medium contains: bovine liver powder 15.0g; malt extract powder 3.0g; yeast extract powder 10.0g; sodium chloride 5.0g; agar 20.0g; porphyrin iron 0.02g; g; nalidixic acid 0.05; chrysone 0.005g; vitamin K 1 0.05g; asparagine 0.01g; sterile defibrinated sheep blood 90ml; add distilled water to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; calcium lactate; Add sterilized porphyrin iron; sterilized nalidixic acid; sterilized vitamin K 1 ; Mix the aseptic defibrated sheep blood evenly, dilute it with sterilized distilled water to 1000ml, and dispense it.

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PUM

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Abstract

The invention discloses a nocardia asteroid selective medium for sputum samples and belongs to the field of detection of microculture. The nocardia asteroid selective medium is characterized in that 1000 ml of culture medium comprises ox liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferroporphyrin, calcium lactate, nalidixic acid, Incarvillea argute ketone, vitamin K1, casein phosphopeptides and sterile defiber sheep blood and the balance of distilled water. Compared with the prior art, the nocardia asteroid selective medium has the advantages of reliability in detection and high separation efficiency of nocardia asteroid.

Description

technical field [0001] The invention relates to the field of test microorganism culture, in particular to a selective culture medium for Nocardia stellatus of sputum samples. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Separation of desired microorganisms from confounding microorganisms. Actinomyces eppingeri is an aerobic soil saprophytic bacterium, a type of actinomycetes, and an opportunistic pathogen. Onset, it can also invade through skin wounds or traumatic wounds, causing human nocardiosis. Nocardiosis asteroids mainly causes human primary and suppurative lung infections through the respiratory tract, and symptoms of pulmonary tuberculosis can occur. Lung lesions can metastasize to the subcutaneous tissue, forming abscesses, ulcers, an...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 王均梅
Owner 王均梅
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