54 results about "Ferroporphyrin" patented technology

The invention discloses a method for producing mRNA (messenger ribonucleic acid) template plasmids by fermenting escherichia coli. When the recombinant escherichia coli is fermented and cultured, a seed solution of the recombinant escherichia coli is inoculated into a basic culture medium to be fermented and cultured until the OD600 value of fermentation liquor is 18-22, then a fed-batch culture medium is continuously added at the feeding rate of 5-15 mL / L / h to be subjected to continuous fed-batch fermentation culture, the basic culture medium contains 0.05-0.2 mmoL / L of ferric salt compound and 0.05-0.2 mmoL / L of copper chloride dihydrate, and the basic culture medium contains 0.05-0.2 mmoL / L of ferric salt compound and 0.05-0.2 mmoL / L of copper chloride dihydrate. The basal culture medium contains 10-15 g / L of copper chloride dihydrate and 100-300 mg / L of p-hydroxybenzoic acid, the carbon source of the basal culture medium contains 0.1-0.3 mmoL / L of a ferric salt compound, 0.1-0.3 mmoL / L of copper chloride dihydrate and 100-300 mg / L of p-hydroxybenzoic acid, the carbon source of the fed-batch culture medium contains 360-480 g / L of glycerol, and the ferric salt compound is one or more of ferrous citrate, ferric ammonium citrate or ferrous sulfate heptahydrate. According to the method, the yield and superhelix content of the mRNA template plasmids are improved by optimizing the culture medium formula and culture conditions, and large-scale production of the high-quality target mRNA template plasmids is facilitated.

The invention belongs to the technical field of bioactive peptides, and discloses a white snakehead oligopeptide as well as a preparation method and application thereof. The white snakehead oligopeptide is selected from one of oligopeptides with amino acid sequences as shown in SEQ ID NO.1-8. The invention further discloses a preparation method of the white snakehead oligopeptide. The polypeptide provided by the invention has a relatively high ferrous / zinc absorption rate to a human body, has a certain function of treating iron-deficiency anemia and zinc deficiency, and has a good application prospect in mineral (iron / zinc) supplements, functional foods and health-care products.

The application discloses a method for synthesizing tervalent tetraaryl iron porphyrin from pyrrole, aromatic aldehyde and ferrous salt, which comprises the following steps: adding anhydrous aluminum chloride, aromatic aldehyde, pyrrole and ferrous salt into DMF under stirring, and then heating and refluxing the reaction mixture for 0.5 hours under nitrogen; removing the nitrogen and continuing to heat the reaction mixture for a certain time; stopping the reaction; cooling the reaction mixture; and placing the reaction mixture at 273K overnight; and finally filtering the reaction mixture to obtain the iron porphyrin.

The application relates to the technical field of sialic acid application, in particular to application of sialic acid in promoting iron absorption of iron supplement agents. It is found for the first time that sialic acid with a daily intake of 0.8-3.5 mg / kg and ferrous iron with a daily intake of 0.2-0.5 mg / kg can promote the absorption of ferrous iron by human bodies. More specifically, the application claims an iron supplement agent containing ferrous iron drugs and sialic acid. The iron supplement agent provided by the application promotes iron supplement by compounding sialic acid and ferrous iron drugs, thereby improving the bioavailability of iron, regulating immunity, promoting intestinal health and achieving various biological activities while supplementing iron.

There is provided a method for producing ferrous sulphate and phosphoric acid comprising treating ferrous phosphate with sulphuric acid for obtaining ferrous sulphate and phosphoric acid. Additionally, there is provided a use of sulphuric acid for treating ferrous phosphate for obtaining ferrous sulphate and phosphoric acid.

In an iron (II) citrate monohydrate, identified by the formula FeC6H6O7-H2O ( Ferrous citrate monohydrate or iron citrate (II) monohydrate), obtained according to a particular production method, citric acid and elemental iron are bound in a molecular complex where the iron is agglomerated in the citrate which moves to a cyclic form and the iron is bound to the O groups of the carboxylic groups 1 and 3 through ionic bonds, forming two enantiomers, D and L (R and S), both perfectly water-soluble and highly assimilable by the human body. Formula (L) & Formula (D). With a different absorption mechanism from traditional iron molecules, said iron citrate monohydrate is usable in pharmaceutical and / or nutraceutical and / or food compositions, in particular in the treatment and prevention of iron deficiency anemias and in general of all diseases and disorders associated with iron deficiency. The effectiveness is proven by several examples.

The invention relates to the field of compound preparation, and discloses a method for preparing alkali metal ferrocyanide by using iron powder as an iron source through a mechanochemical method, and the method comprises the following steps: mixing the iron powder with a solution containing alkali metal cyanide to obtain a mixture, and enabling the water content of the mixture to be not more than 50wt%, the alkali metal cyanide comprises sodium cyanide or potassium cyanide, the reaction time is short, the reaction temperature is low, the process of treating the raw materials in advance is omitted, hydrolysis of the sodium cyanide is reduced, side reactions are reduced, a small amount of NH3 is released in the complexing reaction process, and the method is suitable for industrial production. The yield is high, sulfate containing ferrocyanide is not produced as a byproduct, and unreacted metal residues are recycled. Compared with a traditional method, the method disclosed by the invention is low in production cost and high in added value of products, and has a relatively good industrial prospect.

The utility model discloses a pneumatic stirring device for preparing ferrous chloride, and relates to the technical field of ferrous chloride preparation. The device comprises a tank body, the top of the tank body is fixedly provided with a top cover through bolts, the bottom of the tank body is fixedly connected with an inclined filter pipe, one side of the filter pipe is fixedly communicated with a horizontal discharge pipe, the bottom of the filter pipe is open and is in threaded connection with a bottom cover, and the top of the bottom cover is fixedly connected with a supporting column. A filter cylinder is fixedly connected to the top of the supporting column, a gap is formed between the filter cylinder and the inner wall of the filter pipe, and a limiting ring is fixedly connected to the top of the filter cylinder and makes contact with the inner wall of the filter pipe. According to the utility model, the filter cartridge is taken down by taking down the bottom cover, so that iron powder which is not completely reacted can be conveniently recycled and reused, and meanwhile, during discharging, the stirring shaft drives the movable shaft to rotate, and the movable shaft stirs in the filter cartridge, so that the filter cartridge is prevented from being blocked, and the discharging efficiency is improved.

A method for preparing magnetic amino-functionalized biochar and its application are disclosed, relating to the field of biochar preparation technology. The method involves dissolving ferrous chloride dihydrate, ferric chloride dihydrate, and ammonia in deoxygenated water, stirring until homogeneous, then adding amino-functionalized biochar. After thorough stirring, the product is collected by magnetic adsorption and finally washed to obtain magnetic amino-functionalized biochar. The magnetic amino-functionalized biochar is used for removing chromium from water. This invention provides a method for preparing magnetic amino-functionalized biochar and its application.

The application discloses a method for preparing pure chromium trichloride and ferrous chloride by using low-silicon ferrochrome, and comprises the following steps: an acid leaching step: crushing ferrochrome raw materials with silicon content less than 1% to 1-5mm, adding hydrochloric acid for step-by-step leaching to obtain a leaching solution; a purification step: diluting the leaching solution to a total content of Cr and Fe of 20-80g / L, adding a purifying agent for stirring, standing and filtering to obtain a purified solution; a concentration step: performing negative pressure vacuum concentration on the purified solution, filtering most of sodium chloride when the concentration is 1 / 4-1 / 3 of the original volume, then adding components according to the content of chromium and iron in the filtrate and continuing to concentrate, cooling and crystallizing, and drying to obtain pure chromium trichloride and ferrous chloride. The application significantly improves the filtering performance in the purification process, reduces the loss of chromium and iron elements and the use cost of the purifying agent, and overcomes the problems of battery charge-discharge efficiency reduction and ion membrane blockage caused by the high silicon ion concentration in the electrolyte solution.

A composition and method for capturing and releasing cells containing CD44 antigen, comprising cholesterol-aptamers, silver nanoparticle-modified aptamers, and bleomycin-ferrous ion complexes, exhibits higher biocompatibility and stability, facilitating the formation of a biointerface for the efficient capture and release of mesenchymal stem cells. Applying the composition of this invention to the electrochemical detection of cell capture and release offers advantages such as mild reaction conditions, simplicity, speed, and sensitivity, making it easy to integrate with other automated reaction interfaces for commercial development. Furthermore, it can be combined with electrochemical biochip sensor arrays to achieve even more convenient capture, detection, and release of mesenchymal stem cells.

The invention discloses a chlamydomonas reinhardtii source organism iron steady state regulator and application thereof, and belongs to the technical field of biology. The chlamydomonas reinhardtii hydroxyproline-rich glycoprotein or the protein component thereof disclosed by the invention can activate low-oxygen inducible factor signal channels of intestinal tracts, livers and kidneys, and up-regulate the expression of iron absorption transporters such as a divalent metal ion carrier 1, duodenum cytochrome b, membrane iron transporters and ferrous oxidase in intestinal epithelial cells; according to the present invention, the liver hepcidin overexpression caused by inflammation can be inhibited, the generation of erythropoietin in the liver and the kidney can be promoted so as to stimulate the absorption of the body on the iron nutrient, and the compound can be used in the anemia improving health food and the anemia improving drug. The organism iron homeostasis regulator adopted by the invention is derived from microalgae food, so that compared with a conventional artificially synthesized organism iron homeostasis regulator drug, the organism iron homeostasis regulator has the advantage of no toxic or side effect.

In the ferric iron citrate monohydrate defined by the chemical formula FeC6H6O7 H2O (ferrous citrate monohydrate or ferric iron citrate monohydrate) obtained according to a specific production method, the citric acid and the elemental iron are combined in a molecular complex in which the iron is gathered in the citrate moving into a ring-like form and the iron is bound to the O groups of the carboxyls 1 and 3 through ionic bonds, forming two enantiomers D and L (R and S), both of which are completely water-soluble and highly absorbable by the human body. Formula (L) and Formula (D). With a different absorption mechanism than traditional iron molecules, said ferric iron citrate monohydrate can be used in pharmaceutical compositions and / or nutritional compositions and / or food compositions, in particular for the treatment and prevention of iron deficiency anemia and all diseases and conditions associated with iron deficiency. The effectiveness is demonstrated through several examples.

The application discloses a kind of phosphorus-free pre-film agent, which is composed of the following components with mass percentage: N-lauroyl sarcosine 12-18%, zinc salt 15-20%, ferrous salt 7-10%, acrylic acid-itaconic acid copolymer 5-9% and soft water 50-60%. The application also discloses a preparation method of the above-mentioned phosphorus-free pre-film agent and a method for pre-film treatment in circulating cooling water system. The pre-film agent can effectively avoid the problem that traditional phosphorus-containing pre-film agent easily causes water eutrophication. The circulating water after pre-film does not contain phosphorus and can be recycled. Therefore, the pre-film agent does not need to be discharged after pre-film is completed and can be directly transferred to normal operation, thereby saving water resources and cleaning pre-film cost. The pre-film agent has good synergistic effect among components, and forms an organic-inorganic composite film interwoven on the metal surface. The composite film has the characteristics of rapid film formation, compactness, smoothness, uniformity, stable adhesion and strong corrosion resistance.

The invention belongs to the technical field of food hazard reduction, and discloses a magnetic zearalenone adsorbent and a preparation method thereof. The preparation method comprises the following steps: ultrasonically mixing chitosan, a nitrogen source, ferrous chloride, fatty acid and water to obtain a mixture; reacting the mixture under high-temperature and high-pressure conditions, cooling and centrifuging to obtain a precipitate; carrying out repeated freezing and thawing treatment on the precipitate, and after the freezing and thawing treatment is finished, carrying out freeze drying to obtain a freeze-dried substance; and sequentially firing, grinding and sieving the freeze-dried substance to obtain the magnetic zearalenone adsorbent. The magnetic zearalenone adsorbent disclosed by the invention can be used for efficiently and safely reducing zearalenone.

The invention provides a method for recycling and regenerating chlorosuccinimide, and belongs to the technical field of resource recycling. The method comprises the following steps: decoloring the mother liquor after reaction, adding sulfide to enable metal palladium to form palladium sulfide precipitate, performing chlorination reaction on filtrate, ferrous chloride and phosphorus oxychloride, and performing extraction and back extraction to obtain chlorosuccinimide, so that the chlorosuccinimide is recycled and regenerated. The result of the embodiment shows that the melting point of the chlorosuccinimide obtained by the method disclosed by the invention is consistent with a literature value.

The invention relates to a nano preparation based on a ferrous phosphide nano structure and a preparation method and application thereof, and belongs to the technical field of medical nano materials. In order to solve the problems that in the prior art, the curative effect of CDT is limited, the single therapy effect is poor, and effective imaging guidance lacks, the invention provides a nano preparation based on a ferrous phosphide nanostructure, the nano preparation comprises a core layer, a modification layer and a functional layer, the core layer is a hydrophobic ferrous phosphide nanostructure, the modification layer is a PTMP-PMAA polymer, and the functional layer is glucose oxidase. The nano preparation disclosed by the invention induces tumor apoptosis by increasing the ROS level through multiple synergy of chemical kinetics therapy, hunger therapy and photothermal therapy, so that the tumor treatment effect is remarkably improved; the system also has the advantages of precise dual-mode imaging and biological safety, can precisely position the tumor boundary, monitors the distribution and treatment process of the preparation in the tumor, provides a reliable basis for the selection of treatment opportunities, and shows important application prospects in the field of precise tumor treatment.

The invention discloses a lentinan-ferrous complex for improving intestinal flora and oxidative stress as well as a preparation method and application of the lentinan-ferrous complex, and belongs to the technical field of functional foods and medicines. The preparation method comprises the following steps: dissolving lentinan and trisodium citrate in water in proportion, heating to 50-55 DEG C under the protection of nitrogen, slowly dropwise adding a FeSO4 solution, simultaneously maintaining the pH value to be 8-9 by using a NaOH solution, reacting, centrifuging the reaction solution, precipitating with alcohol, washing, and drying to obtain the lentinan. The prepared complex can significantly improve the stability of ferrous ions, and the bioavailability of the complex in a rat body is superior to that of ferrous sulfate and ferrous glycinate. More importantly, the complex has multiple effects of supplementing iron, regulating intestinal flora (such as reducing F / B value), increasing the yield of short-chain fatty acid and relieving oxidative stress and inflammatory response, and can be used for developing a novel iron supplementing preparation with an intestinal microenvironment improving function.

The invention discloses application of an iron-containing substance. The invention finds that the iron-containing substance can be used for preparing the medicine for treating leucopenia and / or thrombocytopenia; the iron-containing substance is selected from myoglobin and a derivative thereof, hemoglobin and a derivative thereof, ferrous sulfate, ferrous fumarate, ferrous gluconate, a polyferose compound, ferrous succinate, iron amino acid chelate, heme, heme iron polypeptide, ferric hydroxide polymaltose, ferrous lactate, ferric pyrophosphate and iron dextran; and one or more of iron hydroxymaltose. The iron-containing substance can effectively increase the number of leukocytes and / or platelets.

The invention discloses an enhanced colorimetric method for rapidly and selectively detecting glutathione and a kit thereof, and belongs to the technical field of biochemical detection. The method comprises the following steps: preparing a prussian blue solution, a Turnish blue solution, a ferrous chloride solution or a ferric chloride solution as a nano-enzyme catalysis solution; mixing the catalytic solution with an ABTS solution and a hydrogen peroxide solution according to a specific proportion to form a blank reaction solution; adding a glutathione-containing to-be-detected sample into the solution to carry out a chromogenic reaction; and determining the content of glutathione by determining the absorbance difference between the blank and the reaction solution to be detected in a specific wavelength range. The method is mainly used for rapid, selective and quantitative detection of glutathione in the fields of food, medicine and biological analysis.

The invention relates to a preparation method of bis (cyclopentadienyl) iron, which comprises the following steps: mixing 5-(trimethylsilyl)-1, 3-cyclopentadiene, a fluorine ion source, a ferrous source and a solvent under a protective atmosphere condition, and reacting to obtain the bis (cyclopentadienyl) iron. According to the preparation method provided by the invention, ferrocene is prepared through one-step reaction, the process route is simple, the reaction condition is mild, the solvent used in the preparation process can be recycled, the pollution in the preparation process is reduced, and the preparation method is less in three wastes, environment-friendly and suitable for large-scale production.

The invention discloses application of NAC in preparation of a medicine for treating temporomandibular joint disorder, and particularly discloses application of the medicine in effectively improving TMD joint tissue oxidation resistance, inhibiting inflammation level, promoting extracellular matrix secretion and inhibiting iron (ferrous) ions and lipid peroxides so as to improve cartilage injury, relieve pain in a condylar process region and improve osteoarthritis. As a clinical common medicine, NAC is low in cost, high in availability, excellent in cell and tissue safety and high in practicability. The medicine can be safely applied to TMD treatment by means of condyle local injection or condyle region skin application (microneedle) and the like, has important significance in clinical medication of TMD, and has good medication value and application prospect.

The application relates to the technical field of water treatment, and discloses an iron-based coordination assembly as well as a preparation method and application thereof. The iron-based coordination assembly comprises ferrous ions as central ions, and perfluoroalkyl sulfonic acid and / or perfluoroalkyl carboxylic acid with a perfluorinated carbon chain having 7 or 8 carbon atoms as ligand compounds; and the iron-based coordination assembly forms an active catalytic system in an aqueous solution with a pH value of 4.0-5.0. The iron-based coordination assembly provided by the application retains the active 3d electron configuration of Fe 2+ , and effectively breaks the dense hydration layer around Fe 2+ by using the extremely strong hydrophobic effect of the carbon-fluorine chain of the ligand compound, so that a hydrophobic microenvironment rich in NO2 ‑ is constructed at the active site, thereby greatly optimizing the electron transfer path, and successfully solving the key bottleneck of slow reaction rate and low mass transfer efficiency in the traditional Fe 2+ nitrite reduction technology.

The invention relates to the technical field of biological medicines, in particular to application of a ferrous ion compound in preparation of a medicine for treating perianal diseases and the medicine. The comprehensive effect of the ferrous ion compound in treatment of perianal inflammation and the like is remarkably superior to the treatment effect of existing positive drugs and high or low-concentration ferrous ions, and in a domestic rabbit model, the remarkable comprehensive effects of resisting inflammation, improving perianal circulation, inhibiting bacteria and promoting tissue repair can be achieved.

The present application relates to modified polymeric ferric sulfate preparation technology and its application field, especially to a kind of preparation method of modified polymeric ferric sulfate for high-efficiency removal of molybdenum in water.Its characterized in that the preparation method of modified polymeric ferric sulfate includes the following steps: under room temperature condition, after the acidification treatment of ferrous sulfate heptahydrate with dilute sulfuric acid solution, oxidizing agent is added to fully react, when the content of ferrous ion reaches the national standard, the reaction is stopped, after aging for a period of time, it is fully mixed with sodium sulfide solution according to certain proportion for 2-10min, and then modified polymeric ferric sulfate can be prepared.The preparation method of modified polymeric ferric sulfate involved in the present application is simple, and the preparation period is short, the prepared product is stable in performance, the reaction condition is mild, safe and without secondary pollution, the removal efficiency of molybdate in aqueous solution is high, the removal efficiency can reach 99.69% under suitable conditions, and it has good application prospect for removing other high-valence toxic ions in aqueous solution.

The present invention discloses a dual-responsive fluorescent probe for ferrous ions and viscosity in a Parkinson's disease model. The probe is an "off-on" fluorescent probe that can respond well to both ferrous ions and viscosity. It has strong specificity, low background interference, high detection sensitivity, and an emission wavelength with good biocompatibility and low biological background fluorescence. It has been successfully applied to fluorescence imaging in living cells and in the brain tissue of nematodes and fruit flies. The present invention also discloses a preparation method and application. The preparation method is simple to operate and the conditions are mild. The fluorescent probe of the present invention has been successfully applied to biological systems including living cells, Caenorhabditis elegans, and fruit flies, and ferrous ion and viscosity levels have been successfully detected using a confocal microscope.

The invention provides ferrous glycinate and a preparation method and application thereof, and relates to the field of feed additives, the chemical formula of the ferrous glycinate is C2H11FeNO9S, the ferrous glycinate has a first coordinated iron structure and / or a second coordinated iron structure, the first coordinated iron structure is a hexa-coordinated octahedral configuration formed by coordination of tetramolecular water, bimolecular glycine carboxyl oxygen atoms and divalent iron, and the second coordinated iron structure is a hexa-coordinated octahedral configuration formed by coordination of bimolecular water, bimolecular sulfate radicals and bimolecular glycine carboxyl oxygen atoms and divalent iron. Ferrous glycinate and sulfate radicals participate in coordination, free sulfate radicals and free ferrous ions do not exist, that is, ferrous glycinate contains complex iron, and the absorption rate of animals on amino acid ferrous sub-complex iron is far higher than that of inorganic salt ferrous sulfate, so that the ferrous glycinate added into the feed can improve the feed utilization rate. And the bioavailability of animals is higher.

The invention belongs to the technical field of fish culture, and particularly relates to application of ferroporphyrin to improvement of hypoxia stress resistance of grass carps. According to experiments, it is found for the first time that reduction of the number of red blood cells and increase of the brittleness of the red blood cells caused by hypoxia can be completely relieved by adding the porphyrin iron into the daily ration of the grass carps, and it is indicated that the porphyrin iron can completely relieve reduction of the number of the red blood cells and increase of the brittleness of the grass carps in the later growth period caused by hypoxia stress.

The present application relates to the technical field of ferrous phosphate production, and in particular to a ferrous sedimentation tank, comprising a frame and a circular cylinder and a conical cylinder, wherein the conical cylinder is mounted on the frame, the top of the conical cylinder is connected to the bottom of the circular cylinder, an annular bracket is provided on the outer peripheral side of the bottom of the circular cylinder, a power mechanism for performing circular motion around the central axis of the circular cylinder is provided on the annular bracket, a rotating portion is provided on the inner side wall of the bottom of the circular cylinder, an electromagnetic suction assembly is provided between the rotating portion and the power mechanism, the electromagnetic suction assembly is used for the power mechanism for moving to drive the rotating portion, a filtering counterweight portion is movably provided on the inner bottom of the conical cylinder, and a scraper strip is provided between the rotating portion and the filtering counterweight portion. The ferrous sedimentation tank of the present application can drive the scraper strip to rotate along the inner circumferential inclined wall of the conical cylinder by starting the power mechanism, automatically cleaning the sediment attached to the inner circumferential inclined wall of the conical cylinder, without the need for staff to enter the sedimentation tank for cleaning work, which is time-saving, labor-saving and safe.