Method for sterilization in chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid

A technology of nalidixic acid and tebuconazole, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve problems such as contamination by bacteria, and achieve favorable preservation, rich germplasm resources, and preparation convenient effect

Active Publication Date: 2015-08-12
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for removing bacteria during the cultivation of Chlamydomonas reinhardtii by mixing tebuconazole and nalidixic acid, so as to solve the problem of bacterial contamination in the current experiments and applications of Chlamydomonas control

Method used

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  • Method for sterilization in chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid
  • Method for sterilization in chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid
  • Method for sterilization in chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid

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Embodiment 1

[0025] Embodiment 1: The technique of this sterilization method is as follows: A. Chlamydomonas containing mixed bacteria pollution is inoculated on the TAP solid medium plate, and carried out at 23 ± 0.5 ° C, 14 / 10 hour light and dark photoperiod, 8000Lx light intensity Cultivate for 3-5 days; B. Prepare a TAP solid medium plate containing 10ug / ml tebuconazole and 15ug / ml nalidixic acid for use; C. Transplant the Chlamydomonas that has grown on the TAP solid medium plate Put the line on the TAP plate containing the mixed antibacterial agent, and culture it for 5-8 days at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity; D. Transfer the Chlamydomonas that has been sterilized Line it onto an ordinary TAP solid medium plate, and culture it for 3-5 days at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity, for subsequent experiments or preservation for future use.

[0026] Specific steps are as follows:

[0027] 1. Activation...

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Abstract

The invention belongs to methods for killing bacteria and fungi in chlamydomonas reinhardtii and discloses a method for sterilization in a chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid. The method includes: A, inoculating chlamydomonas contaminated by mixed bacteria onto a TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in an occulting light period of 14 / 10 and under the light intensity of 8000Lx; C, transferring the chlamydomonas well grown on the TAP solid medium plate to a TAP plate with mixed antibacterial agents, and culturing for 5-8 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx; D, transferring the sterilized chlamydomonas to the common TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx to achieve a standby for subsequent experiments or breed conservation. The method has the advantage that the problem of bacterial contamination in scientific experiment and application process of chlamydomonas is solved by adoption of the mixed antibacterial agents for treating bacterial and fungus contamination of chlamydomonas.

Description

technical field [0001] The invention relates to a method for removing bacteria and fungi of Chlamydomonas reinhardtii, in particular to a method for removing bacteria in the cultivation process of Chlamydomonas reinhardtii by mixing tebuconazole and nalidixic acid. Background technique [0002] Chlamydomonas reinhardtii is a eukaryotic unicellular microalgae with short growth cycle, easy cultivation, annotated genome, mature related genetic biochemical technology and molecular biology technology, heavy metal adsorption capacity, and high oil production. , and is used as a model organism for life science research and application. At present, research on Chlamydomonas, a model of ciliated diseases, and research on biodiesel and biodecontamination have been carried out. However, in the process of scientific research and application, it is inevitable to cause the pollution of Chlamydomonas species during inoculation, and these miscellaneous bacteria pollution will endanger the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王亮陆军郑元林陈慧怡杨丰源
Owner XUZHOU NORMAL UNIVERSITY
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