Enrichment culture solution for detection of Salmonella in poultry eggs
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An enrichment culture, Salmonella technology, applied in microorganism-based methods, microbial determination/inspection, microorganisms, etc., can solve the problems of infertile lethal cell growth, unfavorable rapid detection, and time-consuming, and achieve significant enrichment. effect, saving testing time and improving efficiency
Active Publication Date: 2013-01-09
SICHUAN UNIV
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[0013] The present invention aims to solve the problem that the selective enrichment liquid and selective medium used in the detection of Salmonella in poultry eggs in the prior art are not conducive to the growth of sublethal cells, so that a very low number of sublethally damaged Missed detection of Salmonella occurs, and the existing non-selective and selective two-step enrichment process takes 48 hours, which consumes a lot of time and is not conducive to rapid detection
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Embodiment 1
[0058] A bacterium enrichment culture solution for detection of Salmonella in poultry eggs, characterized in that it comprises the following raw material components:
[0059] According to the content in 1L of the enrichment culture solution:
[0060] Peptone 7g / L
[0061] Sodium chloride 5g / L
[0062] Disodium hydrogen phosphate 7g / L
[0063] Potassium dihydrogen phosphate 0.5g / L
[0064] Brilliant Green 6 mg / L
[0065] Ox bile salt 0.5g / L
[0066] Nalidixic acid 10mg / L
[0067] Skimmed milk powder 15g / L.
Embodiment 2
[0069] A bacterium enrichment culture solution for detection of Salmonella in poultry eggs, characterized in that it comprises the following raw material components:
[0070] According to the content in 1L of the enrichment culture solution:
[0071] Peptone 11g / L
[0072] Sodium chloride 8g / L
[0073] Disodium hydrogen phosphate 9g / L
[0074] Potassium dihydrogen phosphate 1g / L
[0075] Brilliant Green 8 mg / L
[0076] Ox bile salt 1g / L
[0077] Nalidixic acid 14mg / L
[0078] Skimmed milk powder 40g / L.
Embodiment 3
[0080] A bacterium enrichment culture solution for detection of Salmonella in poultry eggs, characterized in that it comprises the following raw material components:
[0081] According to the content in 1L of the enrichment culture solution:
[0082] Peptone 9g / L
[0083] Sodium chloride 6.5g / L
[0084] Disodium hydrogen phosphate 8g / L
[0085] Potassium dihydrogen phosphate 0.75g / L
[0086] Brilliant Green 7mg / L
[0087] Ox bile salt 0.75g / L
[0088] Nalidixic acid 12mg / L
[0089] Skimmed milk powder 27.5g / L.
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Abstract
The invention relates to an enrichment culture solution for the detection of Salmonella in poultry eggs, belonging to the technical field of the enrichment culture solution which can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low in poultry eggs. 1L of the enrichment culture solution comprises the following raw material components by weight: 7-11g of peptone, 5-8g of sodium chloride, 7-9g of disodium hydrogen phosphate, 0.5-1g of potassium dihydrogen phosphate, 6-8mg of brilliant green, 0.5-1g of bile salt, 10-14mg of nalidixic acid and 15-40g of skimmed milk powder. The enrichment culture solution can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low; and the enrichment processing steps of Salmonella in foods are reduced, the defect that the leak detection of a small amount of sublethal injured Salmonella in a sample can be overcome and the detection reliability and accuracy of Salmonella in foods can be increased.
Description
technical field [0001] The invention relates to a selective enrichment culture solution for the detection of Salmonella in poultry eggs, more specifically, the invention relates to a selective enrichment culture solution for the detection of extremely low numbers of Salmonella with sublethal damage in poultry eggs The culture solution belongs to the technical field of the enrichment culture solution for selectively enriching very low numbers of sublethal damaged Salmonella in poultry eggs. technical background [0002] salmonella( Salmonella enterica ) is a global zoonotic foodborne pathogen. Globally, between 200 million and 1.3 billion people are infected with nontyphoidal Salmonella each year, with an estimated 3 million deaths. There are many types of Salmonella, and more than 2,500 types have been found in the world, among which Salmonella typhimurium and Salmonella enteritidis are the greatest threat to human health. [0003] The total output of poultry eggs in my c...
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