Nocardia asteroid selective medium for sputum specimen
A technology of Nocardia astrocytoma and selection medium, which is applied in the field of testing microorganism culture, can solve the problems of death, low positive rate, slow growth of Nocardia stellariformis, etc., and achieves high separation rate and reliable detection. Effect
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Embodiment 1
[0028] Example 1, the formula for preparing 1000ml medium contains: beef liver powder 15.0g; malt extract powder 2.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; porphyrin iron 0.01g; g; nalidixic acid 0.05g; chrysotrione 0.001g; vitamin K 1 0.1g; sterile defibrated sheep blood 100ml; add distilled water to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; Iron; Sterilized Nalidixic Acid; Sterilized Vitamin K 1 ; Mix the aseptic defibrated sheep blood evenly, dilute it with sterilized distilled water to 1000ml, and dispense it.
Embodiment 2
[0029] Example 2, the formula for preparing 1000ml medium contains: bovine liver powder 10.0g; malt extract powder 5.0g; yeast extract powder 8.0g; sodium chloride 5.0g; agar 15.0g; porphyrin iron 0.01g; g; nalidixic acid 0.03g; chrysotrione 0.003g; vitamin K 1 0.01g; complexin phosphopeptide 0.1g; sterile defibrinated sheep blood 80ml; add distilled water to 1000ml. Preparation method Weigh beef liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; calcium lactate; Ferromorph; Sterilized Nalidixic Acid; Sterilized Vitamin K 1 ; Sterilized Collin Phosphopeptide; Sterile defibrinated sheep blood mixed evenly, sterilized distilled water to 1000ml before packing.
Embodiment 3
[0030] Example 3, the formula for preparing 1000ml medium contains: bovine liver powder 15.0g; malt extract powder 3.0g; yeast extract powder 10.0g; sodium chloride 5.0g; agar 20.0g; porphyrin iron 0.02g; g; nalidixic acid 0.05; chrysone 0.005g; vitamin K 1 0.05g; asparagine 0.01g; sterile defibrinated sheep blood 90ml; add distilled water to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; calcium lactate; Add sterilized porphyrin iron; sterilized nalidixic acid; sterilized vitamin K 1 ; Mix the aseptic defibrated sheep blood evenly, dilute it with sterilized distilled water to 1000ml, and dispense it.
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