Blood ammonia determination and kit thereof

A diagnostic kit and blood ammonia technology are applied in the field of medical examination and determination, which can solve the problems of undiscovered blood ammonia determination method, high recovery rate, influence of accuracy and precision, etc., and achieve low test cost, easy operation, and test results. precise effect

Inactive Publication Date: 2006-03-15
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so it is rarely used at present; the ion exchange method is more accurate than the diffusion method, and the CV is 8%-13%. Selective electrode method is to use NH 3 Diffusion to the surface of the electrode, causing the pH of the electrode to change for meas...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment one (single agent)

[0057] The blood ammonia diagnostic kit of the present embodiment comprises:

[0058] Buffer 80mmol / l

[0059] 2-ketoglutarate 4mmol / l

[0060] Reduced coenzyme 0.2mmol / l

[0061] Glutamate dehydrogenase 40000U / l

[0062] Stabilizer 50% (total volume)

[0063] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the measured blood ammonia sample to reagent is 1 / 25, and the reaction direction is negative reaction .

[0064] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0065] Ammonium ion + 2-ketoglutarate + reduced coenzyme glutamate dehydrogenase glutamic acid

[0066] +coenzyme+water

[0067] The final reactant is placed under a biochemical analysis instrument, and the degree of decrease in the absorbance at the main wavelength o...

Embodiment 2

[0069] Embodiment two (two doses)

[0070] The blood ammonia diagnostic reagent of the present embodiment has:

[0071] Reagent I

[0072] Buffer 100mmol / l

[0073] 2-ketoglutarate 7mmol / l

[0074] Reduced coenzyme 0.25mmol / l

[0075] Stabilizer 35mmol / l

[0076] Reagent II

[0077] Buffer 100mmol / l

[0078] Glutamate dehydrogenase 120000U / l

[0079] Stabilizer 50% (total volume)

[0080] When measuring blood ammonia content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 340nm, the secondary wavelength of the test is above 405nm, the volume ratio of the blood ammonia sample to the reagent is 1 / 25, and the reaction direction is negative reaction.

[0081] The specific measurement steps are:

[0082] Ammonium ion + 2-ketoglutarate + reduced coenzyme glutamate dehydrogenase glutamic acid

[0083] +coenzyme+water

[0084] The final reactant is placed under a biochemical...

Embodiment 3

[0086] Embodiment three (three doses)

[0087] The blood ammonia diagnostic reagent of the present embodiment is three doses, has:

[0088] Reagent I

[0089] Buffer 120mmol / l

[0090] 2-ketoglutarate 10mmol / l

[0091] Stabilizer 50mmol / l

[0092] Reagent II

[0093] Buffer 120mmol / l

[0094] Reduced coenzyme 0.3mmol / l

[0095] Stabilizer 50mmol / l

[0096] Reagent III

[0097] Buffer 120mmol / l

[0098] Glutamate dehydrogenase 200000U / l

[0099] Stabilizer 50% (total volume)

[0100] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the measured blood ammonia sample to reagent is 1 / 25, and the reaction direction is negative reaction .

[0101] The specific measurement steps are:

[0102] Ammonium ion + 2-ketoglutarate + reduced coenzyme glutamate dehydrogenase glutamic acid

[0103] +coenzyme+water

[0104]...

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PUM

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Abstract

A diagnostic kit of blood ammonia consists of buffer solution, 2 ¿C ketoglutarate, reduced coenzyme, glutamate dehydrogenase and stabilizer. Its measuring method includes mixing sample with reagent in certain volume ratio to let them generate enzyme couple reaction, placing reacted matter under biochemical analyzer for detecting absorbance variation of main wavelength so as to measure and calculating out content of blood ammonia.

Description

technical field [0001] The invention relates to a method for measuring blood ammonia content, and at the same time, the invention also relates to a blood ammonia diagnosis kit, which belongs to the technical field of medical examination and measurement. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so it is rarely used at present; the ion exchange method is more accurate than the diffusion method, and the CV is...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/31G01N21/33
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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