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Measurement method of ammonia content in serum

A determination method and serum technology, which are applied in the preparation of test samples, measurement of color/spectral characteristics, etc., can solve the problems of cumbersome, difficult to popularize and use in hospitals, poor stability, etc., and achieve accurate measured values, easy to popularize and use, and stable good effect

Inactive Publication Date: 2014-01-29
DALIAN CITY SHAHEKOU DISTRICT SMALL MEDIUM & MICRO ENTERPRISES SERVICE CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is unstable in aqueous solution and prone to oxidation-reduction reactions, which shortens the validity period of the reagent and is difficult to promote and use in hospitals.
The blood ammonia dry tablet preparation has solved the shortcoming of the poor stability of the liquid dosage form, but the preparation of the reagent requires a freeze-drying process, and the application requires a reconstitution process, which is cumbersome and increases the cost of the reagent.

Method used

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  • Measurement method of ammonia content in serum
  • Measurement method of ammonia content in serum
  • Measurement method of ammonia content in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of reagents and detection: Prepare reagent a and reagent b according to the concentration of each component in Table 1, mix reagent a, reagent b and serum samples at a ratio of 120-240:40:10-20, and test serum samples on a biochemical analyzer Ammonia content in the blood, or detected on a UV spectrophotometer.

[0027] Table 1

[0028] Reagent a component

Embodiment 2

[0030] Prepare reagents and test: prepare reagent a and reagent b according to the concentration of each component in Table 2, mix reagent a, reagent b and serum samples at a ratio of 120-240:40:10-20, and test serum samples on a biochemical analyzer Ammonia content in the blood, or detected on a UV spectrophotometer.

[0031] Table 2

[0032] Reagent a component

[0033] Sodium EDTA

Embodiment 3

[0035] Prepare reagents and test: Prepare reagent a and reagent b according to the concentration of each component in Table 3, mix reagent a, reagent b and serum samples at a ratio of 120-240:40:10-20, and test serum samples on a biochemical analyzer Ammonia content in the blood, or detected on a UV spectrophotometer.

[0036] table 3

[0037] Reagent a component

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Abstract

The invention discloses a method for measuring ammonia content in serum and namely discloses a dual reagent for measuring ammonia content in serum. Accelerated destruction tests of the reagent at 37 DEG C and 42 DEG C show that the reagent is good in stability, so that the complex problem that a freeze-drying agent needs freeze-drying and re-dissolving is solved; the reagent can be prepared when being used; the reagent is capable of stabilizing for 20 months at 2-8 DEG C. Clinical correlation tests show that the method is stable in measurement value, high in accuracy, applicable to manual, semi-automatic and automatic detection systems, and easy to popularize and apply.

Description

technical field [0001] The invention relates to a method for detecting ammonia content in serum, which belongs to the field of in vitro diagnosis. Background technique [0002] The content of free blood ammonia in normal people is extremely low, and the normal value is (10-52) μmol / L. Blood ammonia can be divided into endogenous ammonia and exogenous ammonia according to its source. High blood ammonia content has neurotoxicity. The determination of blood ammonia is very important for liver cirrhosis, portal hypertension, hepatic coma, Reye's syndrome and some congenital metabolic disorders in pediatrics. It is of great significance in the diagnosis, observation and prognosis of disorders and other diseases. [0003] The glutamate dehydrogenase method is easier to operate than other methods and is suitable for automatic biochemical analyzers. The reaction principle is: ammonia reacts with α-KG and NADH under the action of glutamate dehydrogenase to generate L-glutamic acid ...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28G01N1/38
Inventor 秦宏佳
Owner DALIAN CITY SHAHEKOU DISTRICT SMALL MEDIUM & MICRO ENTERPRISES SERVICE CENT
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