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Liquid monomer reagent for determining content of ammonia in blood serum, preparation method and application thereof

A single reagent, liquid technology, applied in the field of detection of ammonia content in serum, to achieve the effect of ensuring accuracy, ensuring stability, and accurate measurement

Inactive Publication Date: 2013-03-27
BEIJING LEADMAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese invention patent application with the application number 200410043900.0 discloses a dry powder reagent for the determination of blood ammonia by enzymatic method, with a stable period of two years; the Chinese invention patent application with the application number 200410041963.2 discloses a liquid reagent, which adopts different enzyme reaction principles , but does not involve the expiration date of the reagent

Method used

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  • Liquid monomer reagent for determining content of ammonia in blood serum, preparation method and application thereof
  • Liquid monomer reagent for determining content of ammonia in blood serum, preparation method and application thereof
  • Liquid monomer reagent for determining content of ammonia in blood serum, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of liquid single reagent for blood ammonia detection

[0027] Dissolve Tris buffer, glutamate dehydrogenase, α-ketoglutarate, reduced nicotinamide adenine dinucleotide (NADH), glucose, glucose dehydrogenase and stabilizer TritonX-100 in distilled water; The dosage or concentration of each component in every liter of monomer reagent is shown in Table 1.

[0028] Table 1

[0029] components

Embodiment 2

[0030] Example 2 Preparation of liquid single reagent for blood ammonia detection

[0031] Dissolve Tris buffer, glutamate dehydrogenase, α-ketoglutarate, reduced nicotinamide adenine dinucleotide (NADH), glucose, glucose dehydrogenase and stabilizer TritonX-100 in distilled water; The dosage or concentration of each component in every 1 liter of monomer reagent is shown in Table 2.

[0032] Table 2

[0033] components

Embodiment 3

[0034] Example 3 Preparation of Liquid Single Reagent for Detection of Blood Ammonia

[0035]Dissolve CAPSO buffer, glutamate dehydrogenase, α-ketoglutarate, reduced nicotinamide adenine dinucleotide (NADH), glucose, glucose dehydrogenase and stabilizer TritonX-100 in distilled water; The dosage or concentration of each component in every 1 liter of monomer reagent is shown in Table 3.

[0036] table 3

[0037] components

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Abstract

The invention discloses a liquid monomer reagent for determining the content of ammonia in blood serum, a preparation method and an application thereof. The liquid monomer reagent comprises the following components: buffering solution, glutamate dehydrogenase, alpha-oxoglutarate, reduction type nicotinamide adenine dinucleotide, glucose, glucose dehydrogenase and a stabilizing agent, wherein the enzyme activity ratio of the glutamate dehydrogenase and the glucose dehydrogenase is (3-3000):1. Shown by accelerating destruction test at the temperature of 37 DEG C and 42 DEG C, the blood-ammonia liquid monomer reagent is good in stability, solves the fussy problem that a lyophilized agent needs to be lyophilized and redissolved, can be prepared and used at any time, and can be stable for 18 mouths at the temperature of 2-8 DEG C; and shown by clinic correlation test, the liquid monomer reagent is stable in blood-ammonia determination, is high in determined-value accuracy, is applicable to manual, semi-automatic and automatic detection system and is easily promoted and used in all hospitals.

Description

technical field [0001] The present invention relates to a reagent for detecting ammonia content, in particular to a monomer reagent for measuring ammonia content in serum. The present invention further relates to a preparation method of the monomer reagent and its application in detecting ammonia content in serum, which belongs to the category of ammonia content in serum. detection field. Background technique [0002] The content of free blood ammonia in normal people is extremely low, and the normal value is (10-47) μmol / L. Blood ammonia can be divided into endogenous ammonia and exogenous ammonia according to its source. Endogenous ammonia mainly comes from the amino acids produced during the metabolism of proteins in the body and ammonia decomposed by deamination, while exogenous ammonia mainly comes from the bacterial decomposition of protein foods in the intestinal tract. The liver is the organ that mainly participates in the removal of ammonia. Therefore, when ammoni...

Claims

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Application Information

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IPC IPC(8): C12Q1/32
Inventor 不公告发明人
Owner BEIJING LEADMAN BIOCHEM
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