Xylooligosaccharide with low DP (degree of polymerization) as well as preparation method and application of xylooligosaccharide

A technology of xylo-oligosaccharide and low polymerization degree, which is applied in the field of xylo-oligosaccharide with low polymerization degree and its preparation, can solve problems such as limiting the scope of application, improve product purity, reduce chromaticity and by-product generation, and save production cost effect

Pending Publication Date: 2017-05-10
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the initial raw material in this preparation method is xylan, which is purified by ethanol precipitation, ultrafiltration, nanofiltration column chromatography, etc., which limits the application range of this technology

Method used

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  • Xylooligosaccharide with low DP (degree of polymerization) as well as preparation method and application of xylooligosaccharide
  • Xylooligosaccharide with low DP (degree of polymerization) as well as preparation method and application of xylooligosaccharide
  • Xylooligosaccharide with low DP (degree of polymerization) as well as preparation method and application of xylooligosaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Take 450g of cottonseed hulls, mix it with 0.05% sodium hydroxide solution at a mass ratio of 1:7, heat up to 60 degrees Celsius and keep it warm for 30 minutes, filter out the liquid; wash it with water until the pH value is neutral; mix it with process water at a ratio of 1:3 The mass ratio is mixed, and the weak acid catalyst (acetic acid) of 0.7% cottonseed hull dry weight percentage is added, steam explosion treatment, processing pressure 1.5-2.5MPa, time 120s; The xylose content in the steam explosion liquid is 5.85%, cooling for stand-by. Add 0.8g xylanase to the cooking liquid, enzymolysis reaction temperature is 60°C, enzymolysis time is 8h, enzymolysis solution is deenzyme, activated carbon decolorization, electrodialysis desalination, concentration to prepare xylooligosaccharides with low polymerization degree. The decolorization temperature used for the decolorization of activated carbon is 50-90°C, the amount of activated carbon added is 0.1-2%, the decolori...

Embodiment 2

[0043] Take 450g of corncobs, mix them with process water at a mass ratio of 1:6 and heat up to 80 degrees Celsius for 40 minutes, filter out the liquid; add acetic acid with a percentage of dry weight of corncobs of 0.8%, and cook at high temperature. The treatment conditions are: cooking temperature 165 ℃, the cooking time is 50min, the xylan in the corn cob is dissolved, and the xylose content in the cooking liquid is 15.75%, and the temperature is lowered for later use. Add 0.1g xylanase to the cooking liquid, enzymolysis reaction temperature is 80°C, enzymolysis time is 8h, enzymolysis solution is deenzyme, activated carbon decolorization, electrodialysis desalination, concentration to prepare xylooligosaccharides with low polymerization degree. The decolorization temperature used for the decolorization of activated carbon is 50-90°C, the amount of activated carbon added is 0.1-2%, the decolorization time is 20-90min, and the decolorization is completed after plate and fra...

Embodiment 3

[0048] Get 450g of wheat straw, mix it with process water in a mass ratio of 1:10 and heat up to 90 degrees centigrade for 60 minutes, filter out the liquid; mix it with process water in a mass ratio of 1:8, add wheat straw dry weight percentage of 1.2% acetic acid, high temperature Cooking treatment, the treatment conditions are: cooking temperature 165 ° C, cooking time 60 minutes, xylan in the wheat straw is dissolved, and the content of xylose in the cooking liquid is 19.96%, and the temperature is lowered for use. Add 0.4g xylanase to the cooking liquid, enzymolysis reaction temperature is 80°C, enzymolysis time is 8h, enzymolysis solution is deenzyme, activated carbon decolorization, electrodialysis desalination, concentration to prepare xylooligosaccharides with low polymerization degree. The decolorization temperature used for the decolorization of activated carbon is 50-90°C, the amount of activated carbon added is 0.1-2%, the decolorization time is 20-90min, and the d...

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Abstract

The invention discloses a preparation method of xylooligosaccharide with low DP (degree of polymerization). The preparation method is characterized by comprising steps as follows: (1) wood fiber biomass is taken as a raw material and subjected to diluted acid / diluted base / hot water pretreatment, a liquid is filtered, a solid phase is treated in a high-temperature degrading or steam explosion pretreatment manner, and the wood fiber biomass is cooled for later use; (2) xylanase is added to a pretreatment solution in step (1) for an enzymatic hydrolysis reaction; (3) an enzymatic hydrolysis solution is treated through enzyme deactivation, purification, concentration and drying, and xylooligosaccharide with low DP is prepared. The DP of finally obtained xylooligosaccharide is low by reasonably optimizing each process step, and xylobiose, xylotriose and xylotetraose are taken as main components, so that the functionality of xylooligosaccharide as a bifidus factor is enhanced. Tests verify that prepared xylooligosaccharide has a good proliferative effect on bifidobacterium animalis and lactobacillus casei. The process is simplified, the product quality is improved, and xylooligosaccharide has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of functional sugar alcohol production, and in particular relates to a xylooligosaccharide with a low degree of polymerization and its preparation method and application. Background technique [0002] Xylo-oligosaccharides are formed by hydrolysis of β-1,4 glycosidic bonds by β-1,4 endoxylanase. Xylo-oligosaccharides can act as a bifidus factor to multiply the beneficial bacteria bifidobacteria in the human intestinal tract, improve the balance of the digestive tract flora of organisms (human and animals), promote the growth of beneficial bacteria in the digestive tract, and inhibit the reproduction of harmful microorganisms. Promote nutrient absorption and improve the body's immunity. [0003] Xylobiose in xylooligosaccharides is a sugar substance that cannot be digested and absorbed by the human body but can be fermented. It has the ability to proliferate bifidobacteria. Xylotriose is formed by connecting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C12P19/00C12N1/20C12R1/01C12R1/245
CPCC12N1/20C12P19/00C12P19/04C12P19/14
Inventor 程少博肖林李莹夏蕊蕊刘宪夫覃树林宋光明王燕
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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