Method for preparing xylooligosaccharide from lignocellulose
A technology of lignocellulose and xylooligosaccharides, which is applied in the food field, can solve environmental pollution and other problems, and achieve the effects of high enzymatic hydrolysis reaction efficiency, increased surface area, and excellent extraction rate
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Embodiment 1
[0021] A method for preparing xylooligosaccharides from lignocellulose, the method comprising:
[0022] (1) Superfine grinding of corncobs
[0023] After the corn cob is dried, it is first coarsely pulverized in a universal pulverizer, and then pulverized in a superfine pulverizer for more than 15 minutes to obtain corn cob powder with a particle size range of 0.1 μm-5 μm;
[0024] (2) Hydrothermal pretreatment
[0025] Put 100g of corncob powder obtained in step (1) and 600mL of water in a reaction kettle, conduct hydrothermal pretreatment at 190°C for 60min, cool and filter to obtain the extract;
[0026] (3) Enzymolysis reaction
[0027] Add 1 g of xylanase to 100 mL of the extract, shake well, and place it at 50°C for 30 minutes to hydrolyze to obtain a hydrolyzate;
[0028] (4) Enzyme inactivation
[0029] The hydrolyzed solution was kept at 90° C. for 20 minutes, cooled and centrifuged, and the supernatant was collected to obtain xylooligosaccharide extract.
Embodiment 2
[0031] A method for preparing xylooligosaccharides from lignocellulose, the method comprising:
[0032] (1) Superfine grinding of corncobs
[0033] After the corn cob is dried, it is first coarsely pulverized in a universal pulverizer, and then pulverized in a superfine pulverizer for more than 15 minutes to obtain corn cob powder with a particle size range of 0.1 μm-5 μm;
[0034] (2) Hydrothermal pretreatment
[0035] Put 100g of corncob powder obtained in step (1) and 1500mL of water in a reaction kettle, conduct hydrothermal pretreatment at 150°C for 50min, cool and filter to obtain the extract;
[0036] (3) Enzymolysis reaction
[0037] Add 0.5 g of xylanase to 100 mL of the extract, shake well, and hydrolyze at 55°C for 20 minutes to obtain a hydrolyzate;
[0038] (4) Enzyme inactivation
[0039] The hydrolyzed solution was kept at 90° C. for 20 minutes, cooled and centrifuged, and the supernatant was collected to obtain xylooligosaccharide extract.
Embodiment 3
[0041] A method for preparing xylooligosaccharides from lignocellulose, the method comprising:
[0042] (1) Superfine grinding of corncobs
[0043] After the corncobs are dried, they are first coarsely pulverized in a universal pulverizer, and then ultrafine pulverized in a superfine pulverizer for more than 15 minutes to obtain corncob powder with a particle size ranging from 0.1 μm to 5 μm;
[0044] (2) Hydrothermal pretreatment
[0045] Put 100g of corn cob powder obtained in step (1) and 500mL of water in a reaction kettle, conduct hydrothermal pretreatment at 190°C for 20min, cool and filter to obtain an extract;
[0046] (3) Enzymolysis reaction
[0047] Add 1.5 g of xylanase to 100 mL of the extract, shake well, and place it at 50°C for 30 minutes to obtain a hydrolyzate;
[0048] (4) Enzyme inactivation
[0049] The hydrolyzed solution was kept at 85° C. for 15 minutes, cooled and centrifuged, and the supernatant was collected to obtain xylooligosaccharide extract....
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Abstract
Description
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Application Information
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