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Method for preparing xylooligosaccharide from lignocellulose

A technology of lignocellulose and xylooligosaccharides, which is applied in the food field, can solve environmental pollution and other problems, and achieve the effects of high enzymatic hydrolysis reaction efficiency, increased surface area, and excellent extraction rate

Inactive Publication Date: 2018-04-03
XUCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional pretreatment methods include partial hydrolysis under acid or alkaline conditions to remove lignin, and it has also been reported that ionic liquids are used to improve the solubility of cellulose. However, these methods tend to decompose lignocellulose into monosaccharides in large quantities. At the same time, the use of acid or alkali also has certain pollution to the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing xylooligosaccharides from lignocellulose, the method comprising:

[0022] (1) Superfine grinding of corncobs

[0023] After the corn cob is dried, it is first coarsely pulverized in a universal pulverizer, and then pulverized in a superfine pulverizer for more than 15 minutes to obtain corn cob powder with a particle size range of 0.1 μm-5 μm;

[0024] (2) Hydrothermal pretreatment

[0025] Put 100g of corncob powder obtained in step (1) and 600mL of water in a reaction kettle, conduct hydrothermal pretreatment at 190°C for 60min, cool and filter to obtain the extract;

[0026] (3) Enzymolysis reaction

[0027] Add 1 g of xylanase to 100 mL of the extract, shake well, and place it at 50°C for 30 minutes to hydrolyze to obtain a hydrolyzate;

[0028] (4) Enzyme inactivation

[0029] The hydrolyzed solution was kept at 90° C. for 20 minutes, cooled and centrifuged, and the supernatant was collected to obtain xylooligosaccharide extract.

Embodiment 2

[0031] A method for preparing xylooligosaccharides from lignocellulose, the method comprising:

[0032] (1) Superfine grinding of corncobs

[0033] After the corn cob is dried, it is first coarsely pulverized in a universal pulverizer, and then pulverized in a superfine pulverizer for more than 15 minutes to obtain corn cob powder with a particle size range of 0.1 μm-5 μm;

[0034] (2) Hydrothermal pretreatment

[0035] Put 100g of corncob powder obtained in step (1) and 1500mL of water in a reaction kettle, conduct hydrothermal pretreatment at 150°C for 50min, cool and filter to obtain the extract;

[0036] (3) Enzymolysis reaction

[0037] Add 0.5 g of xylanase to 100 mL of the extract, shake well, and hydrolyze at 55°C for 20 minutes to obtain a hydrolyzate;

[0038] (4) Enzyme inactivation

[0039] The hydrolyzed solution was kept at 90° C. for 20 minutes, cooled and centrifuged, and the supernatant was collected to obtain xylooligosaccharide extract.

Embodiment 3

[0041] A method for preparing xylooligosaccharides from lignocellulose, the method comprising:

[0042] (1) Superfine grinding of corncobs

[0043] After the corncobs are dried, they are first coarsely pulverized in a universal pulverizer, and then ultrafine pulverized in a superfine pulverizer for more than 15 minutes to obtain corncob powder with a particle size ranging from 0.1 μm to 5 μm;

[0044] (2) Hydrothermal pretreatment

[0045] Put 100g of corn cob powder obtained in step (1) and 500mL of water in a reaction kettle, conduct hydrothermal pretreatment at 190°C for 20min, cool and filter to obtain an extract;

[0046] (3) Enzymolysis reaction

[0047] Add 1.5 g of xylanase to 100 mL of the extract, shake well, and place it at 50°C for 30 minutes to obtain a hydrolyzate;

[0048] (4) Enzyme inactivation

[0049] The hydrolyzed solution was kept at 85° C. for 15 minutes, cooled and centrifuged, and the supernatant was collected to obtain xylooligosaccharide extract....

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Abstract

The invention relates to a method for preparing xylooligosaccharide from lignocellulose. The method comprises the following steps: performing superfine pulverization on the raw material lignocelluloses, performing hydrothermal pretreatment, performing enzymolysis reaction and performing enzyme deactivation. The xylobiose content of xylooligosaccharide extracting liquid is high, the extraction rateof the xylooligosaccharide is more excellent than that of an alkali treatment scheme, pollution is avoided, the enzymolysis reaction efficiency is high, and a new technical scheme is provided for industrialized application of the lignocellulose such as corncobs.

Description

technical field [0001] The invention belongs to the technical field of food, and in particular relates to a method for preparing xylooligosaccharides from lignocellulose. Background technique [0002] Lignocellulose is an abundant and cheap renewable resource. Lignocellulose-rich biomass mainly includes agricultural biomass (such as corn straw, wheat straw, rice straw, and bagasse) and forestry biomass (poplar, According to trees and pine trees, etc.), its main components include cellulose, hemicellulose and lignin. According to statistics, only 3.5% of the biomass on the earth is used by humans, and most of the biomass resources have not been developed and utilized by humans. They are left to fend for themselves in the growth environment. If the biomass resources on the earth can be effectively used, human Possess an inexhaustible and inexhaustible treasure house of resources. With the shortage of petroleum resources and people's increasing attention to energy and environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/00
CPCC12P19/00C12P19/12C12P19/14C13K13/00
Inventor 孙军涛张智超肖付刚王德国张永清魏泉增兰天璐詹静薛文龙
Owner XUCHANG UNIV
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