Method of extracting xylo-oligosaccharide from bamboo shoot shells and separating to obtain xylo-oligosaccharide monomer

A technology of xylooligosaccharides and bamboo shoot shells, applied in the field of biochemistry, can solve problems such as the separation method of xylooligosaccharide monomers that have not been seen, and achieve the effects of low cost, simple and fast operation, and improved extraction rate

Active Publication Date: 2014-05-14
QINGDAO BZ OLIGO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Similarly, there is currently no rapid, economical and ef...

Method used

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  • Method of extracting xylo-oligosaccharide from bamboo shoot shells and separating to obtain xylo-oligosaccharide monomer
  • Method of extracting xylo-oligosaccharide from bamboo shoot shells and separating to obtain xylo-oligosaccharide monomer
  • Method of extracting xylo-oligosaccharide from bamboo shoot shells and separating to obtain xylo-oligosaccharide monomer

Examples

Experimental program
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Embodiment 1

[0036] 1. Method

[0037] 1. Extraction of xylooligosaccharides

[0038] Weigh bamboo shoot shells and add 10 times the volume of 6mol / L NaOH solution, soak at 60°C for 12h, wash with water until the pH value is about 8.0, and dry in an oven at 60°C until constant weight. Accurately weigh 2g of the dried sample into the digestion tank, add 50ml of distilled water, cover and seal it, and then degrade it in the digestion furnace. The microwave power is 700W, and the digestion time is 8min. The supernatant was taken as the crude xylan solution, and the xylan content was determined therein. Then add xylanase to the crude xylan solution, the amount of enzyme added is 35 IU / g xylan, enzymatically hydrolyze in a constant temperature shaking box at 50°C for 8 hours, and take the supernatant for storage. The DNS method was then used to measure the sugar content in the supernatant after enzymolysis, and at the same time determine the content of each xylooligosaccharide.

[0039] 2. M...

Embodiment 2

[0066] 1. Method

[0067] 1. Extraction of xylooligosaccharides

[0068] Weigh bamboo shoot shells and add 10 times the volume of 6mol / L NaOH solution, soak at 60°C for 12h, wash with water until the pH value is about 8.0, and dry in an oven at 60°C until constant weight. Accurately weigh 2g of the dried sample into the digestion tank, add 50ml of distilled water, cover and seal it, and then degrade it in the digestion furnace. The microwave power is 600W, and the digestion time is 10min. The supernatant was taken as the crude xylan solution, and the xylan content was determined therein. Then add xylanase to the crude xylan solution, the amount of enzyme added is 60 IU / g xylan, enzymolyze it in a constant temperature shaking box at 50°C for 6 hours, and take the supernatant for storage. The DNS method was then used to measure the sugar content in the supernatant after enzymolysis, and at the same time determine the content of each xylooligosaccharide.

[0069] 2. Mobile pha...

Embodiment 3

[0094] 1. Method

[0095] 1. Extraction of xylooligosaccharides

[0096] Weigh bamboo shoot shells and add 10 times the volume of 6mol / L NaOH solution, soak at 60°C for 12h, wash with water until the pH value is about 8.0, and dry in an oven at 60°C until constant weight. Accurately weigh 2g of the dried sample into the digestion tank, add 50ml of distilled water, cover and seal it, and degrade it in the digestion furnace. The microwave power is 800W, and the digestion time is 6min. After cooling, take it out, and finally dilute it to 100ml with distilled water The supernatant was taken as the crude xylan solution, and the xylan content was determined therein. Then add xylanase to the crude xylan solution, the amount of enzyme added is 10IU / g xylan, enzymolyze it in a constant temperature shaking box at 50°C for 10h, and take the supernatant for storage. The DNS method was then used to measure the sugar content in the supernatant after enzymolysis, and at the same time deter...

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Abstract

The invention relates to a method of extracting xylo-oligosaccharide from bamboo shoot shells and separating to obtain a xylo-oligosaccharide monomer. The method comprises the following steps: (a) after performing alkaline degradation on the bamboo shoot shells, performing microwave digestion, and adding xylanase to perform enzymolysis to obtain a liquid supernatant; (b) balancing the volume of three active carbon columns by using 50-70% ethanol at a flowing speed of 2-3ml/min, then balancing the volume of the three active carbon columns by using deionized water at a speed of 2-3ml/min, wherein the diameters of the active carbon columns are 3.5 centimeters, the heights of the active carbon columns are 45 centimeters, and active carbon used for chromatography, which is added into the active carbon column is 85g; (c) adding the xylo-oligosaccharide liquid supernatant, eluting 1-3 columns by using the deionized water at a flowing speed of 2-3ml/min, linearly eluting 7-10 columns by using 0.1-37% ethanol at the same flowing speed of 2-3ml/min, and collecting an ethanol eluting part. The method is high in xylo-oligosaccharide extraction rate, easy to operate and good in repeatability; a high-purity monomer can be obtained by separating a xylo-oligosaccharide water solution with low contents of xylobiose to xylopentaose.

Description

【Technical field】 [0001] The invention relates to the technical field of biochemistry, in particular to a method for extracting xylooligosaccharide from bamboo shoot shells and separating and obtaining xylooligosaccharide monomers. 【Background technique】 [0002] Bamboo shoots are one of the traditional green vegetables in China, and they have been regarded as "treasures in dishes" since ancient times in my country. With the popularization of the high-yield technology of bamboo shoot forest and dual-use bamboo shoot forest, the output of fresh bamboo shoots in my country is increasing year by year. Bamboo shoot shells, also known as bamboo sheaths (bambusa spp), are bulk wastes in bamboo shoot processing. Bamboo shoot shells are rich in cellulose (the main component is polypentose), with a content of about 35.08%, which is equivalent to the content of hemicellulose in corn cobs, the main raw material for the production of xylooligosaccharides. A better source of sugar. ...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/00C12P19/12C07H3/04C07H3/06C07H1/08
Inventor 张斌管昶王静王丽丽张娜娜
Owner QINGDAO BZ OLIGO BIOTECH
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