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Application of trichoderma pleuroticum strain ZJ-03 in deep processing of industrial cannabis sativa

A technology of ZJ-03, Trichoderma pedicularis, applied in the field of food processing, to achieve the effect of increasing added value, simplifying the preparation process, saving time and cost

Active Publication Date: 2020-08-18
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the use of Trichoderma pleurotus fermentation for the preparation of syrup in the deep processing of industrial hemp has not been reported; and how to produce xylanase with high enzyme activity and how to prepare high-purity healthy syrup products has become a major issue in the food processing industry. technical problems within

Method used

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  • Application of trichoderma pleuroticum strain ZJ-03 in deep processing of industrial cannabis sativa
  • Application of trichoderma pleuroticum strain ZJ-03 in deep processing of industrial cannabis sativa
  • Application of trichoderma pleuroticum strain ZJ-03 in deep processing of industrial cannabis sativa

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Experimental program
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specific Embodiment approach 1

[0019] Specific embodiment one: the application of Trichoderma pleurotus strain ZJ-03 in the deep processing of industrial hemp in this embodiment is completed according to the following steps:

[0020] One. Under aseptic conditions, inoculate the spores of Trichoderma pleurotus ZJ-03 in the PDA slant medium, and then place them in a mold incubator for cultivation to obtain activated spores of Trichoderma pleurotus ZJ-03; The spores of Trichoderma pleurotus ZJ-03 strain were removed, dispersed and shaken to obtain a concentration of 1.5×10 7 A / mL~2.0×10 7 Individual / mL Trichoderma Pleurotus ZJ-03 spore suspension;

[0021] 2. Add the hemp crumb powder and the hemp seed meal powder into the nutrient solution, stir well and put it into a container for sterilization, and then inoculate the Trichoderma pleurotus ZJ-03 spore suspension obtained in step 1 under aseptic conditions , the inoculum amount is 1.5% to 2%; after inoculation, place it in a sterilized fermentation room, fi...

specific Embodiment approach 2

[0027] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the hemp crumb powder described in step 2 is prepared according to the following steps: crush the hemp crumbs, pass through a 40-mesh sieve, and take the sieve to obtain the hemp powder Hemp crumb powder; hemp seed meal powder is prepared according to the following steps: crush the hemp seed meal, pass through a 40-mesh sieve, and take the sieve to obtain the hemp seed meal powder; the nutrient solution is prepared according to the following steps: mix glucose mother liquor and Su Acidic acid mother liquor is added to distilled water, mixes evenly, obtains nutrient solution, the ratio of the quality of described glucose mother liquor and threonine mother liquor to the volume of distilled water is (0.5g~1g): (0.5g~1g): 100mL .

[0028] Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0029] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the cultivation described in step one: cultivated at 28°C to 30°C until the spores of Trichoderma pleurotus ZJ-03 are mature; described in step two Sterilization: put it into a container after fully stirring, and sterilize at 121°C for 2 hours; extraction described in step 2: add the solid fermentation material of Trichoderma pleurotus to pure water, and stand at 35°C-37°C Extraction for 6h-8h; centrifugation described in step 2: centrifuge the extracted solid-liquid mixture at 3500r / min-4000r / min for 10min-15min.

[0030] Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses an application of a trichoderma pleuroides strain ZJ-03 in deep processing of industrial cannabis sativa, and relates to the technical field of food processing. The invention aims to solve the problems of how to induce trichoderma pleuroides to produce xylanase with high enzyme activity and how to prepare a high-purity healthy syrup product with xylobiose and xylotriose asmain components. The method comprises the following steps: adding China hemp scrap powder and China hemp seed meal powder into a nutrient solution, stirring, and inoculating trichoderma pleuroticum ZJ-03 spore suspension; inoculating, putting into a fermentation chamber, culturing to obtain a trichoderma pleuroides solid fermentation material, performing standing and leaching to obtain a leached solid-liquid mixture, roughly filtering, centrifuging, and taking a supernatant to obtain a crude sugar solution; sequentially carrying out primary evaporation and concentration, primary ion exchange,secondary evaporation and concentration, secondary ion exchange and tertiary evaporation and concentration on the crude sugar solution to obtain a functional syrup product. The trichoderma pleuroticumstrain ZJ-03 can be obtained and applied to deep processing of industrial cannabis sativa.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to the application of Trichoderma pleurotus strain ZJ-03 in deep processing of industrial hemp. Background technique [0002] Industrial hemp (hemp), a plant of the genus Moraceae, refers to a plant that has been artificially selected and approved (identified or registered) as a crop variety. The THC content of the top leaves and flower spikes of the plant group during the flowering period is less than 0.3% (dry Percentage of substances), hemp varieties that no longer have the value of extracting drugs or directly using them as drugs. [0003] The sustainable development of the hemp industry has become an urgent problem to be solved. Modern food machinery and equipment integrate its deep processing into the food industry, opening up a broader development space for hemp, deepening the industrial chain and enhancing the value of hemp. In the international market, there are mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12P19/00C12P19/14C12N1/14C07H3/04C07H3/06C07H1/06C12R1/885
CPCC07H1/06C07H3/04C07H3/06C12N1/14C12P19/00C12P19/12C12P19/14
Inventor 钱朋智张梅娟
Owner QIQIHAR UNIVERSITY
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