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Method for preparing low polyxylose through squeezing-assisted wheat bran enzymolysis

A technology of wheat bran and xylooligosaccharides, which is applied in the development of functional food additives, nutrition and health care, and agricultural and sideline products. It can solve problems such as complex processes, dark product colors, and environmental pollution, so as to benefit human health and increase economic benefits. and the effect of social benefits

Inactive Publication Date: 2011-03-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this process is that the process is more complicated, pollutes the environment, and the product has a dark color (browning after xylan alkali treatment), and simultaneously discards ferulic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0030] Embodiment two: the present invention utilizes extrusion-assisted enzymatic hydrolysis of wheat bran to prepare the method for xylo-oligosaccharide adopting the following process steps:

[0031] Sieve the wheat bran to remove impurities and some starch mixed with the bran after wheat milling. Accurately weigh 50 g of wheat bran under a 35-mesh sieve, suspend it in 500 ml of water, control the temperature of the solution at 40 ° C, and process it with an ultrasonic cell disruptor with an ultrasonic power of 400 W and an ultrasonic time of 60 min.

[0032] Use 1mol / l hydrochloric acid solution to adjust the pH to 5.6, then add 4ml / kg bran high-temperature resistant α-amylase, stir the mixture in a boiling water bath at 98°C for 30min, use iodine solution to check whether the starch has reacted completely, if it turns blue, continue React until the iodine solution does not turn blue; after the suspension is cooled to 50°C, use 1mol / l sodium hydroxide to adjust the pH to 9.0,...

Embodiment 3

[0035] Embodiment three: the present invention utilizes extrusion-assisted enzymatic hydrolysis of wheat bran to prepare the method for xylooligosaccharide adopting the following process steps:

[0036] Sieve the wheat bran to remove impurities and some starch mixed with the bran after wheat milling. Accurately weigh 50 g of wheat bran under a 35-mesh sieve, suspend it in 600 ml of water, control the solution temperature at 50 ° C, and process it with an ultrasonic cell disruptor with an ultrasonic power of 500 W and an ultrasonic time of 8 min.

[0037] Use 1mol / l hydrochloric acid solution to adjust the pH to 5.6, then add 4ml / kg bran high-temperature resistant α-amylase, stir the mixture in a boiling water bath at 100°C for 30min, use iodine solution to check whether the starch has reacted completely, if it turns blue, continue React until the iodine solution does not turn blue; after the suspension is cooled to 50°C, use 1mol / l sodium hydroxide to adjust the pH to 9.0, the...

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Abstract

The invention relates to a method for preparing xylo-oligosaccharide which utilizes and extrudes auxiliary enzymolysis wheat bran, in specific belongs to the technology field of agricultural and sideline product development, functional food additive and nutrition healthcare. With the method, wheat bran is taken as the main raw material and the bran is crushed and enzymolysis is used for removing the starch and protein for preparing the wheat bran meal fiber; and then the fiber passes through a double-screw extruder and enzymolysis is implemented with bacillus subtilis xylanase; the conditionsfor the processes of extrusion and enzymolysis are confirmed; the supernatant liquid after the process of enzymolysis is condensed and dried for preparing the xylo-oligosaccharide. The xylo-oligosaccharide yield with the invention is 11 percent (wheat bran is taken for calculation) and the purity exceeds 70 percent; moreover, content of xylo-disaccharide, xylo- trisaccharide and xylo- tetrasaccharide is more than 50 percent; the wheat bran can be effectively appreciated and utilized and the prepared xylo-oligosaccharide is characterized by high purity, excellent biological activity, excellentbifidobacterium multiplication effect, low heat energy and excellent surface activity.

Description

technical field [0001] The invention relates to a method for preparing xylooligosaccharides by extruding assisted enzymatic hydrolysis of wheat bran, and specifically belongs to the technical fields of development of agricultural and sideline products, functional food additives and nutrition and health care. Background technique [0002] Wheat bran is a by-product of wheat processing, which has the advantages of rich nutrition and low price. At present, wheat bran is mostly used as feed and brewing auxiliary materials. It is cheap and has low economic value. However, wheat bran contains a large amount of physiologically active dietary fiber, and cellulose and hemicellulose are high-quality sources of dietary fiber. As a feed component, its economic value has declined significantly. There have been many reports in foreign countries that wheat bran is very beneficial to human health as a dietary fiber for human beings. In recent years, research on wheat bran has mainly focuse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/00
Inventor 陈正行张海波沈国强杨春霞
Owner JIANGNAN UNIV
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