Bacillus safensis H1, culture method and application thereof

By optimizing the culture conditions of Bacillus salsa H1, a strain of Bacillus salsa H1 with high antibacterial activity and environmental adaptability was provided, which solved the problem of insufficient inhibition of Gram-positive drug-resistant bacteria in the existing technology, and realized the effective inhibition of Gram-positive bacteria and the preparation of drug sensitivity tablets.

CN122344537APending Publication Date: 2026-07-07HENAN UNIV OF SCI & TECH +1
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Patent Information

Application Number
CN202610777341.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-01
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

There is a lack of existing Bacillus salsa strains that have high inhibitory activity against Gram-positive drug-resistant bacteria and strong environmental adaptability, and there is limited research on their secondary metabolites.

Method used

A strain of Bacillus sabolicii H1 and its culture method were provided. The liquid fermentation conditions were optimized as follows: yeast powder 5 g/L, peptone 10 g/L, sucrose 37 g/L, pH=10, temperature 25℃, rotation speed 140 rpm, culture time 24 h. The fermentation supernatant with strong environmental adaptability and highly effective antibacterial substances was obtained and used to prepare drug sensitivity tablets and antibacterial drugs.

Benefits of technology

The S. sarcodactylus H1 strain grows stably under neutral to strongly alkaline conditions and secretes antibacterial substances, significantly improving its inhibitory effect on Gram-positive bacteria such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. It has the potential to prepare drug sensitivity tablets and alleviate the problem of antibiotic resistance.

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Abstract

This invention discloses a strain of *Bacillus saeformis* H1, its culture method, and its applications, relating to the field of microbial technology. The strain was deposited on March 31, 2026, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 38117. This strain exhibits strong environmental adaptability, can stably grow under neutral to strongly alkaline conditions, and secretes antibacterial substances with low toxicity and no pathogenicity. After optimization, its optimal liquid fermentation conditions are: 5 g / L yeast extract, 10 g / L peptone, 37 g / L sucrose, pH=10, temperature 25℃, rotation speed 140 rpm, and culture time 24 h. The fermentation supernatant of this strain shows significant inhibitory effects on Gram-positive bacteria such as *Staphylococcus aureus*, methicillin-resistant *Staphylococcus aureus*, and *Streptococcus pneumoniae*, and can be prepared into drug sensitivity tablets. The *Bacillus saeformis* H1 provided by this invention can be used to prepare antibacterial drugs or antibacterial infection drugs, providing a new strategy for alleviating the problem of antibiotic resistance.
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