42 results about "Nucleoside monophosphate" patented technology

The present invention is directed to compounds, compositions and methods for treating or preventing cancer and viral infections, in particular, HIV, HCV, Norovirus, Saporovirus, HSV-1, HSV-2, Dengue virus, Yellow fever, and HBV in human patients or other animal hosts. The compounds are certain 6-substituted purine monophosphates, and pharmaceutically acceptable, salts, prodrugs, and other derivatives thereof. In particular, the compounds show potent antiviral activity against HIV-1, HIV-2, HCV, Norovirus, Saporovirus, HSV-1, HSV-2, Dengue virus, Yellow fever, and HBV.

A crystallizing process for 5'-nucleoside-sodium phosphate includes such steps as proportionally adding the inorganic Na salt and solution educing agent, stirring while crystallizing at 15-40 deg.C, filtering, alcohol washing and vacuum drying. It has high output and controllable size of crystal.

Disclosed are certain cycloSalingenyl pyrimidine nucleoside monophosphates comprising positron emitters or gamma-emitting radiohalides, uses thereof for monitoring Alzheimer's disease progression and evaluating response to therapy and process for their preparation.

The invention provides a plurality of recombinant microorganisms for producing orotic acid and a method thereof. The recombinant microbial strains have one or more or all of the following characteristics: (1) the synthesis of phosphoribosyltransferase or orotic acid nucleoside monophosphate decarboxylase in an orotic acid metabolic pathway is weakened or completely lost; (2) a corresponding enzymeamount is increased through overexpression of five genes of enzymes participating in the orotic acid synthesis route; and (3) the corresponding transport protein is increased by overexpression of a transport protein coding gene for intracellular transport of orotic acid.

The invention provides a bitterness screening agent for eliminating the bitterness of a leaven enzyme extract and a seafood enzyme extract as well as a preparation method of the bitterness screening agent. The bitterness screening agent comprises nucleoside or nucleoside monophosphate, ethyl maltol, a licorice extract and amino acetic acid. According to the invention, substances with different debitterizing effect mechanisms are compounded to enable the agent to achieve an effect of comprehensively screening and shielding the bitter peptides in yeast extracts or seafood hydrolysate, so that the acceptance of substrate is higher, and the application range of the substrate is enlarged.

This invention provides method and device for directly crystallizing nucleoside monophosphate (NMP) from its solution. The process comprises: (1) vaporizing and concentrating crude NMP solution to obtain an oversaturate solution; (2) introducing the oversaturated solution to crystallizer by a heat-insulating pipe, and crystallizing at constant temperature and stirring speed to obtain NMP crystals. The process has such advantages as low cost, high crystal quality, good crystal structure, high crystal purity and high yield.

The invention relates to a kit for diagnosing 5'-nucleotidase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the active concentration of 5'-nucleotidase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, coenzyme, nucleoside monophosphate, glutamine, adenosine diphosphate, glutamine synthetase, glutamate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of 5'-nucleotidase.

The invention relates to a kit for diagnosing 5'-nucleotidase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the active concentration of 5'-nucleotidase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, coenzyme, nucleoside monophosphate, sucrose, sucrose phosphorylase, fructose dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of 5'-nucleotidase.

The invention relates to a kit for diagnosing 5'-nucleotidase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the active concentration of 5'-nucleotidase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, nucleoside monophosphate, an oxaloacetic acid, acetylcoenzyme A, phosphoenolpyruvate carboxykinase, pyruvate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of 5'-nucleotidase.

The invention relates to a 5'nucleotidase diagnostic kit utilizing technologies of an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring the activity concentration of 5'nucleotidase and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, reduced coenzyme, nucleoside monophosphate, adenosine diphosphate, oxaloacetic acid, pyruvate carboxylase, formaldehyde dehydrogenase and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV/visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the activity concentration of the 5'nucleotidase.

The invention relates to a kit for diagnosing 5'-nucleotidase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the active concentration of 5'-nucleotidase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, nucleoside monophosphate, adenosine diphosphate, an oxaloacetic acid, phosphoenolpyruvate carboxykinase, formaldehyde deoxygenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of 5'-nucleotidase.

The invention relates to a kit for diagnosing 5'-nucleotidase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the active concentration of 5'-nucleotidase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, nucleoside monophosphate, adenosine diphosphate, an oxaloacetic acid, ammonium chloride, pyruvate carboxylase, alanine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of 5'-nucleotidase.

The invention discloses a method for preparing nucleoside triphosphate and deoxynucleoside triphosphate from polyphosphate. The method comprises the following steps: (1) preparing nucleoside monophosphate kinase and PPK enzyme required by reaction; (2) reacting to generate NTP and dNTP: adding polyphosphate into a reaction system taking NMP and dNMP as substrates, and reacting the NMP, dNMP and polyphosphate under the combined action of nucleotide monophosphate kinase and PPK enzyme to generate dNTP and NTP; and (3) separating a target product. The high-efficiency and specificity of the biological enzyme of the invention enables the whole synthesis process to be simple, stable and rapid, and the whole process is free of addition of pollutants such as organic solvents, the whole process is green and environment-friendly, the production is safe, the period is short and the cost is low. ATP does not need to be added as an energy source in the reaction, and the raw material cost is saved by 50% or above; there are few by-products generated in the reaction, and it is easy for later purification without the interference of ATP, AMP and ADP; the enzyme dosage is small, and the reaction is rapid.

The invention relates to a kit for diagnosing 5'-nucleotidase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the active concentration of 5'-nucleotidase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, nucleoside monophosphate, glutamine, adenosine diphosphate, a pyruvic acid, glutamine synthetase, alanine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of 5'-nucleotidase.

The invention relates to a kit for diagnosing 5'-nucleotidase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the active concentration of 5'-nucleotidase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, nucleoside monophosphate, glutamine, adenosine diphosphate, a ketoglutaric acid, glutamine synthetase, glutamate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of 5'-nucleotidase.