10 results about "Nucleoside monophosphate" patented technology

This invention discloses a method for the bioenzymatic synthesis of deoxynucleoside monophosphate. The invention provides a mutant protein of dNK, comprising mutating amino acid residues at positions 84, 88, 110, and 172 of the amino acid sequence shown in SEQ ID NO:1, while leaving other amino acid residues unchanged, to obtain a protein with deoxynucleoside kinase activity. This invention obtains a deoxynucleoside kinase mutant capable of stably and efficiently catalyzing the synthesis of 3'-O-NH2-deoxynucleoside monophosphate using 3'-O-NH2-deoxynucleoside as a substrate, providing a new approach and pathway for nucleotide production. The bioenzymatic synthesis of (deoxy)nucleoside monophosphate of this invention has advantages such as high conversion rate, mild reaction conditions, simple production process, environmental friendliness, and ease of large-scale production.

The invention discloses a method for synthesizing deoxynucleoside monophosphate by a biological enzyme method. The mutant protein of dNK provided by the invention is a protein with deoxynucleoside kinase activity, which is obtained by mutating amino acid residues at 84th, 88th, 110th and 172nd positions on an amino acid sequence as shown in SEQ ID NO: 1 and keeping amino acid residues at other positions unchanged. According to the present invention, the deoxynucleoside kinase mutant is obtained, 3 '-O-NH2-deoxynucleoside can be adopted as the substrate, the 3'-O-NH2-deoxynucleoside monophosphate can be stably and efficiently catalyzed and synthesized, and the new thought and the new approach are provided for nucleotide production. The (deoxy) nucleoside monophosphate synthesized by the biological enzyme method has the advantages of high conversion rate, mild reaction conditions, simple production process, environmental friendliness, easiness in large-scale production and the like.

The present invention provides a method for producing nicotinamide mononucleotide, which uses one enzyme to produce nicotinamide mononucleotide from a nucleoside monophosphate, pyrophosphate, and nicotinamide as raw materials. The method for producing nicotinamide mononucleotide according to the present invention includes at least the following steps 1) and 2): 1) A first step of allowing substantially one enzyme to act on a nucleoside monophosphate and pyrophosphate to generate phosphoribosyl diphosphate; 2) A second step of allowing substantially only the above-mentioned one enzyme to act on the phosphoribosyl diphosphate as the product of the first step and nicotinamide to generate nicotinamide mononucleotide.

This application relates to the field of metabolic engineering and genetic engineering technology for production, specifically providing a uracil-producing strain, its construction method, and its application. This strain is... E. coli Knockout of the uridine kinase gene in the W3110 genome udk Simultaneously integrates the T7 RNA polymerase gene; weakens the uridine kinase gene. pyrH Overexpression of carbamoyl phosphate synthase gene carAB ribose-phosphokinase gene prsA pyrimidine-5'-nucleotide nuclease gene ppnN UMP phosphohydrolase gene umpH and umpG Ribonucleotide hydrolase 1 gene rihA and glutamine synthase gene glnA Heterogeneous introduction of wild-type Bacillus subtilis B. subtilis 168 orotate nucleoside monophosphate decarboxylase gene pyrF and orotic acid ribosyltransferase gene pyrE To achieve safe, efficient, and low-cost production of uracil.

The present disclosure generally relates to methods for the enzymatic synthesis of nucleotide triphosphates. In particular, the methods include a one-pot preparation of a modified nucleotide triphosphate starting from 3´-vinyl blocked nucleoside monophosphate.

The present disclosure generally relates to methods for the enzymatic synthesis of nucleotide triphosphates. In particular, the methods include a one-pot preparation of a modified nucleotide triphosphate starting from 3′-vinyl blocked nucleoside monophosphate.

Recombinant microorganisms having reduced activity of endogenous nucleoside monophosphate phosphohydrolases (EC 3.1.3.5 or EC 3.1.3.6), endogenous ribonucleotide monophosphatases (EC 3.1.3.5), endogenous nucleotide triphosphate pyrophosphohydrolases (EC 3.6.1.56), and / or endogenous 5'-ribonucleotide phosphohydrolases (EC 3.1.3.5) relative to parental microorganisms; and engineered to increase activity of a pathway that utilizes cytidine triphosphate (CTP). The recombinant microorganisms can be used in methods for producing isoprenoids and / or methods for increasing recycling of cytidine monophosphate (CMP) to cytidine triphosphate (CTP).

The invention provides a catalytic system. The catalytic system is used for catalyzing nucleoside monophosphate into nucleoside diphosphate; the catalytic system comprises nucleotide kinase, acetate kinase, acetyl phosphate or salts thereof, a phosphoric acid donor, inorganic pyrophosphatase and a solvent. The method has the beneficial effects that a catalytic system of nucleotide kinase, acetate kinase and inorganic pyrophosphatase is simple and efficient, nucleoside monophosphate can be converted into nucleoside diphosphate in one step, and the method is particularly suitable for converting 3, 7-dimethyl guanosine monophosphate into 3, 7-dimethyl guanosine diphosphate. The catalytic system provided by the invention is high in conversion efficiency and can be used for large-scale production.

The application provides a novel energy regeneration system, a cell-free synthesis system, a kit and a method. By using a nucleoside monophosphate or a salt thereof as a main component of the energy regeneration system, a conventional expensive NTP energy system component is replaced, and the problem of high cost of cell-free synthesis is effectively solved.

The invention belongs to the technical field of biosynthesis, and relates to a multi-enzyme catalysis system and method for synthesizing functional glycans. The system comprises a substrate module comprising nucleoside monophosphate, monosaccharide, polyphosphate and acceptor molecules; the nucleoside monophosphoric acid is one or more of uridine monophosphoric acid, adenosine monophosphoric acid, guanosine monophosphoric acid or cytidine monophosphoric acid; an enzyme module comprising a polyphosphate kinase, at least one remedial pathway synthetase, and at least one glycosyltransferase; the cofactor module comprises magnesium ions and optional inorganic pyrophosphatase; the system can realize in-situ regeneration of sugar nucleotide with nucleoside monophosphate as an initial cofactor and one-pot synthesis of target glycan. The invention systematically proposes that cheap NMP is used as a unique or main starting cofactor to replace expensive NTP or NDP for in-situ regeneration of various sugar nucleotides for the first time. Cofactor cost can be reduced by dozens of times, and a foundation is laid for large-scale production of glycans.