5 results about "Nucleoside monophosphate" patented technology

This application relates to the field of metabolic engineering and genetic engineering technology for production, specifically providing a uracil-producing strain, its construction method, and its application. This strain is... E. coli Knockout of the uridine kinase gene in the W3110 genome udk Simultaneously integrates the T7 RNA polymerase gene; weakens the uridine kinase gene. pyrH Overexpression of carbamoyl phosphate synthase gene carAB ribose-phosphokinase gene prsA pyrimidine-5'-nucleotide nuclease gene ppnN UMP phosphohydrolase gene umpH and umpG Ribonucleotide hydrolase 1 gene rihA and glutamine synthase gene glnA Heterogeneous introduction of wild-type Bacillus subtilis B. subtilis 168 orotate nucleoside monophosphate decarboxylase gene pyrF and orotic acid ribosyltransferase gene pyrE To achieve safe, efficient, and low-cost production of uracil.

The present disclosure generally relates to methods for the enzymatic synthesis of nucleotide triphosphates. In particular, the methods include a one-pot preparation of a modified nucleotide triphosphate starting from 3´-vinyl blocked nucleoside monophosphate.

The present disclosure generally relates to methods for the enzymatic synthesis of nucleotide triphosphates. In particular, the methods include a one-pot preparation of a modified nucleotide triphosphate starting from 3′-vinyl blocked nucleoside monophosphate.

The application provides a novel energy regeneration system, a cell-free synthesis system, a kit and a method. By using a nucleoside monophosphate or a salt thereof as a main component of the energy regeneration system, a conventional expensive NTP energy system component is replaced, and the problem of high cost of cell-free synthesis is effectively solved.

The invention belongs to the technical field of biosynthesis, and relates to a multi-enzyme catalysis system and method for synthesizing functional glycans. The system comprises a substrate module comprising nucleoside monophosphate, monosaccharide, polyphosphate and acceptor molecules; the nucleoside monophosphoric acid is one or more of uridine monophosphoric acid, adenosine monophosphoric acid, guanosine monophosphoric acid or cytidine monophosphoric acid; an enzyme module comprising a polyphosphate kinase, at least one remedial pathway synthetase, and at least one glycosyltransferase; the cofactor module comprises magnesium ions and optional inorganic pyrophosphatase; the system can realize in-situ regeneration of sugar nucleotide with nucleoside monophosphate as an initial cofactor and one-pot synthesis of target glycan. The invention systematically proposes that cheap NMP is used as a unique or main starting cofactor to replace expensive NTP or NDP for in-situ regeneration of various sugar nucleotides for the first time. Cofactor cost can be reduced by dozens of times, and a foundation is laid for large-scale production of glycans.