5'-nucleotidase diagnosis reagent kit and 5'-nucleotidase active concentration determination method
A diagnostic kit and nucleotidase technology, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, and color/spectral property measurement, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0044] The 5'-nucleotidase diagnostic reagent of this embodiment is a single reagent, comprising:
[0045] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0046] Stabilizer 500mmol / L
[0047] Reduced coenzyme 0.25mmol / L
[0048] Pyruvate carboxylase 6000U / L
[0049] Alanine dehydrogenase 10000U / L
[0050] Nucleoside monophosphate 8mmol / L
[0051] Adenosine diphosphate 6mmol / L
[0052] Oxaloacetate 12mmol / L
[0053] Ammonium chloride 12mmol / L
[0054] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0055] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume of the tested 5'-nucleotidase sample and reagent The ratio is 1 / 25, the reaction direction is negative reaction (decreasing rea...
Embodiment 2
[0058] The 5'-nucleotidase diagnostic reagent of this embodiment is a double reagent, comprising:
[0059] Reagent 1
[0060] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0061] Stabilizer 50mmol / L
[0062] Reduced coenzyme 0.25mmol / L
[0063] Nucleoside monophosphate 8mmol / L
[0064] Adenosine diphosphate 6mmol / L
[0065] Oxaloacetate 12mmol / L
[0066] Ammonium chloride 12mmol / L
[0067] Reagent 2
[0068] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0069] Stabilizer 500mmol / L
[0070] Pyruvate carboxylase 6000U / L
[0071] Alanine dehydrogenase 10000U / L
[0072] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0073] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, tested 5'-nucleotidase sample and r...
Embodiment 3
[0076] The 5'-nucleotidase diagnostic reagent of the present embodiment is three reagents, comprising:
[0077] Reagent 1
[0078] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0079] Stabilizer 50mmol / L
[0080] Reduced coenzyme 0.25mmol / L
[0081] Nucleoside monophosphate 8mmol / L
[0082] Adenosine diphosphate 6mmol / L
[0083] Oxaloacetate 12mmol / L
[0084] Ammonium chloride 12mmol / L
[0085] Reagent 2
[0086] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0087] Stabilizer 500mmol / L
[0088] Alanine dehydrogenase 10000U / L
[0089] Reagent 3
[0090] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0091] Stabilizer 500mmol / L
[0092] Pyruvate carboxylase 6000U / L
[0093] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0094] When measuring the concentration of 5'-nucleotidase activity, set it on the aut...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com