Primer probe combination for detecting six respiratory viruses, kit and application

Abstract

The invention relates to the field of medical detection, in particular to a primer probe combination for detecting six respiratory viruses, a kit and application. According to the kit provided by theinvention, a method of combining isothermal amplification and a micro-fluidic chip is adopted; the kit can accurately detect novel coronavirus (2019-nCoV) S and N target genes and influenza A virus, novel influenza A H1N1 virus (2009), influenza A H3N2 virus, influenza B virus and respiratory syncytial virus at the same time, and the purpose of distinguishing the novel coronavirus from common influenza virus is achieved. The kit disclosed by the invention has the advantages of multiple detection indexes, simplicity and convenience in operation, short detection time and the like, six virus indexes can be simultaneously detected by one-time sample adding, the time from sample treatment to report is within 1.5 hours, and the kit is more time-saving and labor-saving than qPCR method.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a combination of primers and probes, a kit and an application for detecting six respiratory viruses. Background technique [0002] The initial symptoms of 2019-nCoV patients are mostly fever, fatigue and dry cough, and gradually develop severe symptoms such as dyspnea. Most patients have a good prognosis, and some severe cases may develop acute respiratory distress syndrome or septic shock, and even die. Currently, there is no specific treatment for the disease. The incubation period of the disease is generally 3 to 7 days, the shortest incubation period is one day, and the longest incubation period is 14 days. The incubation period is contagious. Mainly through droplet transmission and contact transmission, there may be a risk of aerosol transmission in closed and unventilated places, and prevention and isolation treatment need to be strengthened. The population is generally...

AI Technical Summary

Problems solved by technology

Although real-time fluorescent PCR technology has the characteristics of high sensitivity and high specificity, the experiment requires high detection personnel and the experimental operation steps require frequent heating and cooling processes, requiring expensive instruments, and light detection takes at least 1.5 hours above, and most of them are single-index detection with low throughput; PCR capillary electrophoresis fragment analysis also has high requirements for detection personnel and frequent and time-consuming experimental steps; although the immunochromatography test strip method has high sensitivity, the detection results may be including false positives and false negatives

Images