Serum-free culture medium for BHK cells

A culture medium, BHK21 technology, applied in cell culture active agent, animal cells, culture process and other directions, can solve the problems of complex serum components, inability to accurately distinguish, and large differences between serum batches.

Active Publication Date: 2017-12-05
上海倍谙基生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an important substance for cell growth and maintenance, serum plays an important role in cell culture, but it also has many problems and drawbacks that cannot be solved so far, such as: (1) The components in serum are complex and cannot be accurately distinguished; (2) There are great differences between serum batches, and the storage period of serum is short (no more than 1 year), which limits the standardization and serialization of batch produc

Method used

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  • Serum-free culture medium for BHK cells
  • Serum-free culture medium for BHK cells
  • Serum-free culture medium for BHK cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0251] Example 1 - Medium I

[0252] A serum-free medium suitable for large-scale suspension culture of BHK21 cells, including amino acids, vitamins, salts, lipids, trace elements, hormones, buffers, protein hydrolyzates and additives, the contents of which are as follows: mg / L

[0253] Copper Sulfate (Cupric Sulfate Pentahydrate) 10.21

[0254] Ferric Nitrate Nonahydrate 30.19

[0255] Ferrous Sulfate Heptahydrate 0.34

[0256] Magnesium Chloride Hexahydrate 31.38

[0257] Magnesium Sulfate 32.44

[0258] Calcium Chloride Dihydrate 154.21

[0259] Potassium Chloride 302.45

[0260] Sodium Chloride (Sodium Bicarbonate) 2010.22

[0261] Dibasic Sodium Phosphate 52.14

[0262] Sodium Dihydrogen Phosphate 54.4

[0263] Alanine (L-Alanine) 5.01

[0264] Arginine (L-Arginine Hydrochloride) 102.21

[0265] Asparagine (L-Asparagine Monohydrate) 21.3

[0266] Aspartic acid (L-Aspartic Acid) 10.2

[0267] Cystine (L-Cysteine ​​Dihydrochloride) 31.21

[0268] Cysteine ​​(L...

Embodiment 2

[0312] Example 2 - Medium II

[0313] A serum-free medium suitable for large-scale suspension culture of BHK21 cells, including amino acids, vitamins, salts, lipids, trace elements, hormones, buffers, protein hydrolyzates and additives, the contents of which are as follows: mg / L

[0314] Copper Sulfate (Cupric Sulfate Pentahydrate) 50.21

[0315] Ferric Nitrate Nonahydrate 198.19

[0316] Ferrous Sulfate Heptahydrate 0.64

[0317] Magnesium Chloride Hexahydrate 108.38

[0318] Magnesium Sulfate 109.44

[0319] Calcium Chloride Dihydrate 194.21

[0320] Potassium Chloride 492.45

[0321] Sodium Chloride (Sodium Bicarbonate)6980.22

[0322] Dibasic Sodium Phosphate 142.14

[0323] Sodium Dihydrogen Phosphate 144.4

[0324] Alanine (L-Alanine) 7.81

[0325] Arginine (L-Arginine Hydrochloride) 192.21

[0326] Asparagine (L-Asparagine Monohydrate) 38.3

[0327] Aspartic acid (L-Aspartic Acid) 28.2

[0328] Cystine (L-Cysteine ​​Dihydrochloride) 49.21

[0329] Cysteine...

Embodiment 3

[0373] Example 3 - Medium III

[0374] A serum-free medium suitable for large-scale suspension culture of BHK21 cells, including amino acids, vitamins, salts, lipids, trace elements, hormones, buffers, protein hydrolyzates and additives, the contents of which are as follows: mg / L

[0375] Copper Sulfate (Cupric Sulfate Pentahydrate) 30.32

[0376] Ferric Nitrate Nonahydrate 119.44

[0377] Ferrous Sulfate Heptahydrate 0.54

[0378] Magnesium Chloride Hexahydrate 71.55

[0379] Magnesium Sulfate 72.49

[0380] Calcium Chloride Dihydrate 174.24

[0381] Potassium Chloride 402.43

[0382] Sodium Chloride (Sodium Bicarbonate) 4600.22

[0383] Dibasic Sodium Phosphate 102.14

[0384] Sodium Dihydrogen Phosphate 104.4

[0385] Alanine (L-Alanine) 7.04

[0386] Arginine (L-Arginine Hydrochloride) 162.21

[0387] Asparagine (L-Asparagine Monohydrate) 31.3

[0388] Aspartic acid (L-Aspartic Acid) 20.2

[0389] Cystine (L-Cysteine ​​Dihydrochloride) 41.21

[0390] Cysteine...

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PUM

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Abstract

The present invention provides a serum-free culture medium for BHK cells, wherein specifically the culture medium comprises amino acids, vitamins, salts, lipids, trace elements, a buffer agent, protein hydrolysates, acid-alkali indicators, and an additive. According to the present invention, with the culture medium, the separation and purification of the product is easily achieved, the quality of the product can be improved, the long-term BHK cell subculture can be supported, the long-term and complex adaptation process is not required, the BHK cell suspension growth can be supported, the preparation and the use of the serum-free culture medium are easy, and the serum-free culture medium is suitable for the large-scale production of biological products.

Description

technical field [0001] The invention relates to the field of cell culture, more specifically, the invention relates to a serum-free medium for BHK21 cell culture and a corresponding culture method. Background technique [0002] Foot-and-mouth disease is a zoonotic disease and a highly contagious viral disease. It is listed as the top animal disease epidemic in the world by the International Veterinary Bureau. Once foot-and-mouth disease breaks out, it can quickly evolve into a biological disaster, and the animal husbandry and related industries will be hit directly and even destructively. A large number of animals will be culled and destroyed, causing serious public health problems and social panic. From 1999 to 2001, foot-and-mouth disease swept the world. More than 50 countries in Asia, Africa, and Europe broke out foot-and-mouth disease one after another. Countries such as Britain, South Korea, and Japan, which had been free of the disease for many years, also fell ill, w...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0686C12N2501/33C12N2501/998C12N2500/32C12N2500/24C12N2500/90
Inventor 谭文松刘旭平
Owner 上海倍谙基生物科技有限公司
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