Method for separating anthocyanins monomer from lycium ruthenicum fruits

Abstract

The invention relates to a method for separating an anthocyanins monomer from lycium ruthenicum fruits. The method comprises the following steps: (1) pretreating raw materials: removing impurities from lycium ruthenicum fruits, washing the lycium ruthenicum fruits with water to obtain the treated lycium ruthenicum fruits; (2) preparing a formic acid-methanol solution at a volume concentration of 2%; (3) adding the formic acid-methanol solution into the lycium ruthenicum fruits for extraction at room temperature to obtain an extract; (4) filtering, and concentrating the extract by evaporating to obtain an lycium ruthenicum anthocyanins extract; (5) treating macroporous adsorption resin; (6) redissolving the lycium ruthenicum anthocyanins extract, applying the redissolved lycium ruthenicum anthocyanins extract to the treated macroporous adsorption resin, and collecting effluent; (7) concentrating the effluent under reduced pressure to obtain crude anthocyanins; (8) injecting the crude anthocyanins into a high-performance semipreparative chromatographic column, and separating to obtain the anthocyanins monomer with the purity of higher than or equal to 90%; concentrating the anthocyanins monomer under reduced pressure, and performing freeze drying in vacuum to constant weight to obtain finished anthocyanins monomer. The method is good in separating effect, short in time consumption and low in cost.

Description

Technical field [0001] The present invention involves a method of separating the chromoside monomer, especially a method of separating the chromoside monomer from black fruit wolfberry fruits. Background technique [0002] Black fruit wolfberry ( Lycium ruthenicum Murray) is a perennial shrub in soraanaceae, which has significant physiological activity.Both the Tibetan medicine classics and "Crystal Beads" and "Four Medical Consterasses" are recorded: black fruit wolfberry is sweet, flat, clear heart fever, used to treat heart fever, heart disease, irregular menstruation, and menopause."Uyghur Medicine" records: Black fruit wolfberry fruit can treat diseases such as urethral stones, ringworm, gum bleeding, etc., and folks are used as nourishing strong, bright eyes and antihypertensive drugs.Black fruit wolfberry is rich in natural pigments, color, and total polyphenols such as Anthocyanins. It has strong antioxidant activity and is a plant with dual -use medicine. In recent years...

AI Technical Summary

Problems solved by technology

[0004] At present, high-speed countercurrent chromatography (HSCCC) is mainly used for the separation of anthocyanins. The limitation is that there are fewer chromatographic peaks, poor separation effect, and long separation time; while high-speed countercurrent chromatography is used to separate anthocyanins. Due to the high polarity of anthocyanins, it is necessary to add methyl tert-butyl ether and n-butanol in the separation system. These two highly polar reagents are difficult to remove in the subsequent concentration process, and even if they can be removed, high temperatures are required. will cause loss of anthocyanins

Furthermore, high-speed countercurrent chromatography is an open separation system, and the chemical reagents, especially methyl tert-butyl ether, are easy to volatilize, causing harm to operators and easy to pollute the environment.

Images