Method for separating anthocyanins monomer from lycium ruthenicum fruits

A technology of black fruit wolfberry and anthocyanin, which is applied in the field of separating and preparing anthocyanin monomers, can solve the problems of volatilization of chemical reagents, harm to operators, loss of anthocyanins, etc., and achieves the advantages of short separation time, safe operation, and prevention of degradation. Effect

Active Publication Date: 2014-03-12
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, high-speed countercurrent chromatography (HSCCC) is mainly used for the separation of anthocyanins. The limitation is that there are fewer chromatographic peaks, poor separation effect, and long separation time; while high-speed countercurrent chromatography is used to separate anthocyanins. Due to the high polarity of anthocyanins, it is necessary to add methyl tert-butyl ether and n-butanol in the separation

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  • Method for separating anthocyanins monomer from lycium ruthenicum fruits
  • Method for separating anthocyanins monomer from lycium ruthenicum fruits
  • Method for separating anthocyanins monomer from lycium ruthenicum fruits

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[0029] Example 1 A method for isolating and preparing anthocyanin monomer from Lycium barbarum fruit, comprising the following steps:

[0030] (1) Raw material pretreatment: select the black fruit wolfberry fruit to remove impurities, wash it with water, and wash away the floating soil on the surface to obtain the processed black fruit wolfberry fruit.

[0031] (2) Prepare a methanol solution with a volume concentration of 2% formic acid: mix 200 mL of formic acid and 9800 mL of methanol evenly.

[0032] (3) Add the formic acid methanol solution obtained in step (2) to the fruit of Lycium ruthenicum fruit, and carry out normal temperature leaching at a ratio of 1 g: 1 mL at room temperature in the dark, and the extraction frequency is 1 time, 12 h each time, and combined to obtain the extract.

[0033] (4) Remove insoluble matter, protein, and polysaccharides from the extract with filter paper to obtain a filtrate, which is vacuum-evaporated on a rotary evaporator at 40...

Example Embodiment

[0042] Example 2 A method for isolating and preparing anthocyanin monomer from Lycium barbarum fruit, comprising the following steps:

[0043] ⑴Raw material pretreatment is the same as Example 1 .

[0044] ⑵Preparation volume concentration is 2% methanol solution of formic acid Example 1 .

[0045] (3) Add the formic acid methanol solution obtained in step (2) to the fruit of Lycium ruthenicum fruit, carry out normal temperature leaching at a ratio of 1 g: 5mL at room temperature in the dark, the extraction times are 3 times, each time for 24 h, combined to obtain extract.

[0046] (4) Remove insoluble matter, protein, and polysaccharides from the extract with filter paper to obtain a filtrate. The filtrate is vacuum evaporated and concentrated to a paste on a rotary evaporator at 50° C. to obtain anthocyanin extract from Lycium barbarum, and methanol is recovered.

[0047] ⑸The treatment of macroporous adsorption resin is the same as Example 1 .

[0048] (6) Re...

Example Embodiment

[0055] Example 3 A method for isolating and preparing anthocyanin monomer from Lycium barbarum fruit, comprising the following steps:

[0056] ⑴Raw material pretreatment is the same as Example 1 .

[0057] ⑵Preparation volume concentration is 2% methanol solution of formic acid Example 1 .

[0058] (3) Add the formic acid methanol solution obtained in step (2) to the fruit of Lycium ruthenicum fruit, and carry out normal temperature leaching at a ratio of 1 g: 3 mL at room temperature in the dark, and the extraction times are 2 times, each time for 18 h, and combined to obtain extract.

[0059] (4) Remove insoluble matter, protein, and polysaccharides from the extract with filter paper to obtain a filtrate, which is vacuum-evaporated on a rotary evaporator at 45°C and concentrated to a paste to obtain anthocyanin extract from Lycium ruthenicum, and recover methanol.

[0060] ⑸The treatment of macroporous adsorption resin is the same as Example 1 .

[0061] (6) R...

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Abstract

The invention relates to a method for separating an anthocyanins monomer from lycium ruthenicum fruits. The method comprises the following steps: (1) pretreating raw materials: removing impurities from lycium ruthenicum fruits, washing the lycium ruthenicum fruits with water to obtain the treated lycium ruthenicum fruits; (2) preparing a formic acid-methanol solution at a volume concentration of 2%; (3) adding the formic acid-methanol solution into the lycium ruthenicum fruits for extraction at room temperature to obtain an extract; (4) filtering, and concentrating the extract by evaporating to obtain an lycium ruthenicum anthocyanins extract; (5) treating macroporous adsorption resin; (6) redissolving the lycium ruthenicum anthocyanins extract, applying the redissolved lycium ruthenicum anthocyanins extract to the treated macroporous adsorption resin, and collecting effluent; (7) concentrating the effluent under reduced pressure to obtain crude anthocyanins; (8) injecting the crude anthocyanins into a high-performance semipreparative chromatographic column, and separating to obtain the anthocyanins monomer with the purity of higher than or equal to 90%; concentrating the anthocyanins monomer under reduced pressure, and performing freeze drying in vacuum to constant weight to obtain finished anthocyanins monomer. The method is good in separating effect, short in time consumption and low in cost.

Description

Technical field [0001] The present invention involves a method of separating the chromoside monomer, especially a method of separating the chromoside monomer from black fruit wolfberry fruits. Background technique [0002] Black fruit wolfberry ( Lycium ruthenicum Murray) is a perennial shrub in soraanaceae, which has significant physiological activity.Both the Tibetan medicine classics and "Crystal Beads" and "Four Medical Consterasses" are recorded: black fruit wolfberry is sweet, flat, clear heart fever, used to treat heart fever, heart disease, irregular menstruation, and menopause."Uyghur Medicine" records: Black fruit wolfberry fruit can treat diseases such as urethral stones, ringworm, gum bleeding, etc., and folks are used as nourishing strong, bright eyes and antihypertensive drugs.Black fruit wolfberry is rich in natural pigments, color, and total polyphenols such as Anthocyanins. It has strong antioxidant activity and is a plant with dual -use medicine. In recent years...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08C09B61/00
Inventor 索有瑞胡娜丁晨旭张秋龙
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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