Micro rate type sensor for bio-assay of salicylic acid as well as construction method and application thereof

A technology of salicylic acid and microfilament, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of complex biological samples, restrictions on practical application, and poor reproducibility, so as to improve stability and reproducibility, and realize accurate Effects on sex and reliability

Active Publication Date: 2018-06-19
BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the electrochemical sensor will inevitably be affected by environmental and human factors during the modification process, and the reproducibility is poor, which seriously restricts its practical application.
However, biological samples in in vivo research are more complex, and it is necessary to improve the reproducibility and reliability of electrochemical sensors.

Method used

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  • Micro rate type sensor for bio-assay of salicylic acid as well as construction method and application thereof
  • Micro rate type sensor for bio-assay of salicylic acid as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Constructing a Miniature Ratio Sensor

[0046] (1) Connect a platinum microwire with a diameter of 100 μm to a copper wire, seal it in a glass tube, then seal the glass tube with insulating glue and epoxy resin, and expose a part of the copper wire as a wire at one end, and expose about 2mm platinum microwire at the other end Make electrodes.

[0047] Boil the platinum microwires in concentrated nitric acid for about 3-10 minutes, then ultrasonically wash them in acetone and double distilled water respectively; 0.7V cyclic voltammetry scan until the ideal cyclic voltammetry is obtained;

[0048](2) GO and acidified MWNT were dissolved in 1 mL ultrapure water at a mass ratio of 1:2, and then 2 mg of thionine was added to the above 1 mL GO / MWNT mixed solution, and sonicated for 4 h. Preparation of nanoclusters of thionine / GO / MWNT. Purify by centrifugation and wash with ultrapure water several times until it is washed, and resuspend the precipitate in 1 mL of ...

experiment example 1

[0053] Collect 4 ratiometric sensors constructed in Example 1 and non-ratiosensors constructed in Comparative Example 1 to compare the 20 detection results of the same concentration of SA. For the statistical results, see figure 2 . The relative standard deviation (RSD) of the non-ratio sensor is 15.4%, while the RSD of the ratio sensor is 5.2%, and the reproducibility is significantly higher than that of the non-ratio sensor. figure 2 Among them, A represents a non-ratio sensor, and B represents a ratio sensor.

experiment example 2

[0054] Experimental example 2 On-line detection of plant salicylic acid in vivo

[0055] The platinum wire microelectrode sensor modified in Example 1 was used to detect SA in sunflower seedlings treated with salt stress in vivo.

[0056] 1) use the microelectrode modified in embodiment 1 as working electrode, Ag / AgCl wire as reference electrode, platinum wire as counter electrode, test SA standard solution by cyclic voltammetry, ratiometric sensor uses the oxidation current of SA and thionine The ratio of the oxidation current corresponds to the concentration of SA to establish a standard curve.

[0057] For the non-ratiometric sensor constructed in Comparative Example 1, a standard curve was established with the oxidation current of SA corresponding to the concentration of SA.

[0058] 2) Taking sunflower seedlings as the test object, set up the control group (0mm NaCl), the treatment group 1 (50mm NaCl) and the treatment group 2 (100mm NaCl).

[0059] The sunflower seedli...

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Abstract

The invention relates to the technical field of micro electrode biology, in particular to a micro rate type sensor for bio-assay of salicyclic acid as well as a construction method and application thereof. An electrochemical sensor generally outputs a single signal of one substance. The invention provides an SA micro rate type sensor capable of outputting two signals, so that the SA micro rate type sensor can be better applied to the viviperception of plants. An electrode is modified by adopting a nano cluster of thionine / GO / MWNT, the thionine is used as a built-in correction signal and formsa dual-signal output together with an SA signal, thereby constructing the rate type sensor, significantly improving the stability and reproducibility of the SA sensor, and improving the accuracy and reliability when in plant in-vivo SA detection.

Description

technical field [0001] The invention relates to the field of microelectrode biotechnology, in particular to a miniature ratiometric sensor for detecting salicylic acid in a living body and its construction method and application. Background technique [0002] Salicylic acid (SA) is an important plant hormone, which plays an important role in various physiological processes of plants such as seed germination, flowering, stomatal closure, membrane permeability and defense response to stimuli. Traditional methods for detecting SA in plants include high-pressure liquid chromatography, gas chromatography-mass spectrometry, enzyme-linked immunosorbent assay, and fluorescence spectroscopy. Among these methods, SA is mainly detected in vitro by collecting plant samples. The collection of samples usually causes great damage or even death to plants, and the in vitro detection usually reflects a static and cumulative effect, which cannot reflect the immediate information of plant horm...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 陈立平李爱学王成王晓冬侯佩臣胡叶何璐璐周航高权陈泉
Owner BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI
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