Manufacture method of silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and application thereof

An immunosensor, iron tetroxide technology, applied in the field of new nano functional materials and biosensors, can solve the problems of expensive equipment, complex equipment, long analysis time, etc., achieve high sensitivity and rapid detection, simple preparation process, The effect of increasing sensitivity
CN102749442AActive Publication Date: 2012-10-24UNIV OF JINAN
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2012-10-24

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Abstract

The invention discloses a manufacture method of a silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and the application thereof. The manufacture method of the electrochemical immunosensor includes modifying thionine-graphene mixed solution on the surface of a glassy carbon electrode, conducting cross linking on an antibiotic antibody incubated by Ag-Fe3O4 mesoporous nanometer particles, closing non-specificity active sites by bovine serum albumin to manufacure the antibiotic electrochemical immunosensor. A detection method of antibiotic is that a reference electrode-saturated calomel electrode, an electrode-platinum filament electrode and a working electrode are correctly connected on an electrochemical working station, and immunodetection is conducted through the square wave voltammetry. The antibiotic electrochemical immunosensor has high sensitivity and selectivity, is simple in detection method and has the advantages of being quick, high in efficiency, goodin specificity, low in cost, convenient to operate and the like. It takes to 2-3 minutes to finish one detection process.
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Description

technical field

[0001] The invention relates to a preparation method and application of a silver hybrid mesoporous iron ferric oxide antibiotic immunosensor. Specifically based on Ag-Fe 3 o 4 A label-free electrochemical immunosensor for detecting various antibiotics constructed by a nanocomposite material belongs to the technical field of new nanometer functional materials and biosensors. Background technique

[0002] Antibiotics are drugs against pathogenic microorganisms. They are a class of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher animals and plants during their life. Other chemical substances that function in the development of living cells.

[0003] Commonly used antibiotics include extracts from microbial culture fluids and chemically synthesized or semi-synthesized compounds, which are divided into the following categories: β -Lactams, aminoglycosides, tetracyclines, chloramphenicols, macrolides, an...

Claims

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