Electrochemical biosensor for organic phosphorus pesticide detection and preparation method of electrochemical biosensor

Abstract

The invention relates to an electrochemical biosensor for organic phosphorus pesticide detection and a preparation method of the electrochemical biosensor. The preparation method comprises the following steps: polymerizing thionine to the surface of a silk-screen printing carbon electrode so as to modify polythionine on the silk-screen printing carbon electrode, fixing acetylcholin esterase and a carboxylation multi-wall carbon nanotube on the surface of the polythionine modified silk-screen printing carbon electrode through chitosan gel, and drying, thereby manufacturing the silk-screen printing carbon electrode, that is, the working electrode of the electrochemical biosensor. The invention provides the electrochemical biosensor which is applied to organic phosphorus pesticide detection and is relatively good in stability, relatively high in sensitivity and relatively low in cost; the invention further provides the preparation method of the electrochemical biosensor for organic phosphorus pesticide detection, the cost of the preparation method is relatively low, and an electrochemical biological bed manufactured by using the electrochemical biosensor is relatively good in stability and relatively high in sensitivity.

Description

technical field [0001] The invention relates to an electrochemical biosensor for detecting organophosphorus pesticides and a preparation method thereof. Background technique [0002] Organophosphorus pesticides are one of the commonly used pesticides in my country, which play an important role in the high yield and harvest of crops, but the problems of pesticide residues in agricultural products and environmental pollution are becoming more and more serious. In order to protect the health of the public, various countries have formulated the maximum limit standards for pesticide residues in agricultural products and food. The detection methods of organophosphorus pesticides mainly include chromatography, spectrophotometry, enzyme inhibition method, electrochemical method, chemiluminescence method and so on. However, the existing rapid detection methods have been unable to meet the needs of on-site rapid detection of large quantities of agricultural products and food-related ...

AI Technical Summary

Problems solved by technology

Most of the above electrochemical enzyme biosensors for organophosphorus pesticides are based on glassy carbon, gold and platinum electrodes, which are expensive and difficult to reuse once damaged

[0006]In addition, enzyme sensors also have deficiencies in actual use. First, the sensitivity, stability and service life still need to be further improved; Easy to lose activity, which is a difficulty affecting the commercial application of enzyme sensors

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