Preparation method and application of SiO2-MWCNTs enzymatic glucose electrochemical sensor

Abstract

The invention discloses a preparation method and application of a SiO2-MWCNTs enzymatic glucose electrochemical sensor, and belongs to the technical field of electrochemical sensors, wherein the preparation method comprises the steps: taking 0.01-0.05 g of SiO2 nano-powder, fully grinding the SiO2 nano-powder, and adding the ground SiO2 nano-powder into a carbon nanotube NMP dispersion liquid; adding 1 ml of deionized water into the carbon nanotube NMP dispersion liquid, continuously vibrating for 2 days by using an ultrasonic cleaning machine, uniformly mixing the ground SiO2 nano-powder withthe carbon nanotube NMP dispersion liquid, and fully dispersing to obtain a SiO2-MWCNTs composite material; weighing 0.0700 g of thionine and adding into a beaker, and adding deionized water at the temperature of 40 DEG C into the beaker for dissolution; pouring the dissolved thionine solution into a 25 mL brown volumetric flask, and fixing the volume to obtain a 0.01 M thionine solution; and taking 0.001 g of chloroauric acid and putting into a beaker, adding deionized water into the beaker, and carrying out sufficient stirring through a glass rod.

Description

technical field [0001] The present invention relates to an electrochemical sensor, in particular to a SiO 2 -MWCNTs has an enzyme-catalyzed glucose electrochemical sensor preparation method and application, and belongs to the technical field of electrochemical sensors. Background technique [0002] Accurate detection of glucose is of great value to biomedicine. In modern medicine, due to the sharp increase in the number of people with diabetes, its threat to human beings is increasing. However, the lack of its diagnosis and treatment technology has always plagued modern medicine. Therefore, it has become a major problem. By detecting the glucose content in the blood or saliva of diabetic patients, the blood sugar concentration can be quickly and accurately judged, and the blood sugar of diabetic patients can be effectively monitored and treated. [0003] At present, there are mainly the following methods for detecting glucose concentration: kinetic spectrophotometry, intern...

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Problems solved by technology

[0003] At present, there are mainly the following methods for detecting glucose concentration: kinetic spectrophotometry, internal standard method of laser Raman spectroscopy, and biosensor assay. Among them, the electrochemical glucose sensor with the advantages of low cost, high reliability, and easy operation is widely used. Since Updike et al. produced the first glucose oxidase biosensor in 1967, it has been widely used by scientists all over the world due to its advantages of simple operation, low production cost, easy miniaturization, high sensitivity, short response time, good selectivity and stability. Research and development, electrochemical glucose sensors are divided into enzyme electrochemical glucose sensors and enzyme-free electrochemical glucose sensors according to different catalytic methods. Among them, enzyme-free glucose electrochemical sensors usually have the characteristics of high sensitivity and high specificity, but their activity It is susceptible to interference from external environments such as humidity, temperature, and pH, which leads to shortcomings such as poor reproducibility and stability in the detection of glucose. By comparing with the enzyme electrochemical glucose sensor, there is an enzyme electrochemical glucose sensor. The sensor has the advantages of low detection limit, wide detection range, good stability, and low cost, making it widely concerned

[0004] In the prior art, the electrode material of the enzyme-catalyzed glucose electrochemical sensor has the disadvantage of being easily affected by other interfering substances such as uric acid and chloride ions in the process of detecting glucose, which makes the final detection result deviate;

[0005] The existing enzymatic glucose electrochemical sensor has a narrow linear range for detecting glucose, which is relatively small compared with the fasting range of normal blood glucose concentration in the human body, which limits its use in the detection of diabetes

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