Double signal-double recognition imprinted electrochemical sensor and preparation method thereof and detection method of ovalbumin

A technology of ovalbumin and detection methods, applied in the field of imprinted electrochemical sensors, can solve the problems of expensive, time-consuming, poor selectivity, etc.

Active Publication Date: 2020-01-14
ANHUI NORMAL UNIV
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  • Abstract
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Problems solved by technology

However, severe matrix interference and low concentrations of complex biological samples present great obstacles for the identification and detection of ovalbumin
[0003] So far, many techniques have been developed for the analysis of ovalbumin, such as enzyme-linked immunosorbent assay, liquid chromatography, etc., but these methods usually have the disadvantages of being expensive, time-consuming, poor selectivity, and low sensitivity

Method used

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  • Double signal-double recognition imprinted electrochemical sensor and preparation method thereof and detection method of ovalbumin
  • Double signal-double recognition imprinted electrochemical sensor and preparation method thereof and detection method of ovalbumin
  • Double signal-double recognition imprinted electrochemical sensor and preparation method thereof and detection method of ovalbumin

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preparation example Construction

[0024] The invention provides a method for preparing a double-signal-double-recognition imprinted electrochemical sensor, the preparation method comprising:

[0025] 1) Perform electrochemical polymerization on the surface of the electrode carrier to form a poly(p-aminobenzenesulfonic acid) film pABSA to obtain pABSA / electrode carrier;

[0026] 2) Carry out electrochemical polymerization on the surface of pABSA / electrode carrier to form polythionine film pTH to obtain pTH / pABSA / electrode carrier;

[0027] 3) placing the pTH / pABSA / electrode carrier in a mixed solution for electrochemical polymerization, and then eluting the electrode to remove ovalbumin OVA to form an imprinted polymer MIP to obtain MIP / pTH / pABSA / electrode carrier;

[0028] Wherein, the mixed solution contains ovalbumin OVA and 3-thiophene boronic acid 3-TBA.

[0029] In step 1) of the above preparation method, the conditions of electrochemical polymerization can be selected in a wide range, but in order to ma...

Embodiment 1

[0049] 1) Preparation of pABSA / GCE

[0050] Immerse the glassy carbon electrode GCE containing 2×10 -4 mol / L ABSA in PBS solution with a pH of 7.0, scan 15 cycles by CV method, the polymerization potential is -1.5~+2.5V, and the scanning speed is 100mV / s, rinse the polymerized electrode with deionized water, and then pABSA / GCE was obtained.

[0051] 2) Preparation of pTH / pABSA / GCE

[0052] Immerse pABSA / GCE in PBS solution with pH 5.0 containing 5mmol / L TH, scan 30 times by CV method, the polymerization potential is -0.4~+0.4V, and the scanning speed is 100mV / s, and use the polymerized electrode Rinse with deionized water to obtain pTH / pABSA / GCE.

[0053] 3) Preparation of imprinted electrochemical sensors

[0054] Immerse pTH / pABSA / GCE in PBS solution at pH 8.0 containing 10mmol / L 3-TBA and 0.4mg / mL OVA, and conduct electropolymerization by CV method: the potential range is -0.2~+1.2V, and the sweep rate is 125mV / s, the number of aggregation circles is 20 circles. Then...

Embodiment 2

[0056] 1) Preparation of pABSA / GCE

[0057] Immerse the glassy carbon electrode GCE containing 1 × 10 -4 mol / L ABSA in PBS solution with a pH of 7.5, scan 18 circles by CV method, the polymerization potential is -1.5~+2.5V, and the scanning speed is 80mV / s, rinse the polymerized electrode with deionized water, then pABSA / GCE was obtained.

[0058] 2) Preparation of pTH / pABSA / GCE

[0059] Immerse pABSA / GCE in PBS solution with pH 5.5 containing 4mmol / L TH, scan 32 circles by CV method, the polymerization potential is -0.4~+0.4V, and the scanning speed is 80mV / s. Rinse with deionized water to obtain pTH / pABSA / GCE.

[0060] 3) Preparation of imprinted electrochemical sensors

[0061] Immerse pTH / pABSA / GCE in a PBS solution with a pH of 8.5 containing 8mmol / L 3-TBA and 0.3mg / mL OVA, and conduct electropolymerization by CV method: the potential range is -0.2~+1.2V, and the sweep rate is 120mV / s, the number of aggregation circles is 22. Then the obtained modified electrode wa...

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Abstract

The invention discloses a double signal-double recognition imprinted electrochemical sensor and a preparation method thereof and a detection method of ovalbumin. The preparation method includes the steps that (1), electrochemical polymerization is conducted on the surface of an electrode carrier to form a poly-p-aminobenzene sulfonic acid film pABSA to obtain a pABSA/electrode carrier; (2), electrochemical polymerization is conducted on the surface of the pABSA/electrode carrier to form a poly thionine film pTH to obtain a pTH/pABSA/electrode carrier; and (3), the pTH/pABSA/electrode carrier is put into a mixed solution for electrochemical polymerization, and then the electrode is eluted to remove ovalbumin OVA to form an imprinted polymer MIP to obtain a MIP/pTH/pABSA/electrode carrier, wherein, the mixed solution contains ovalbumin OVA and 3-thienylboric acid 3-TBA. The double signal-double recognition imprinted electrochemical sensor has excellent sensitivity and selectivity for thedetection of the ovalbumin.

Description

technical field [0001] The invention relates to an imprinted electrochemical sensor, in particular to a dual-signal-double-recognition imprinted electrochemical sensor, a preparation method thereof and a detection method for ovalbumin. Background technique [0002] Ovalbumin is a high-quality protein that is an important part of eggs. Studies have found that eggs can improve human intelligence, reduce cholesterol levels, treat iron deficiency anemia, detoxify and fight cancer. Therefore, it is of great importance to develop highly selective and efficient analytical methods for the identification and quantification of ovalbumin in biological samples. However, severe matrix interference and low concentration of complex biological samples provide great obstacles for the identification and detection of ovalbumin. [0003] So far, many techniques have been developed for ovalbumin analysis, such as enzyme-linked immunosorbent assay, liquid chromatography, etc., but these methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/48
Inventor 阚显文柴绒
Owner ANHUI NORMAL UNIV
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