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Dopamine polymer/noble metal nanoparticle electrochemical sensor as well as preparation method and application thereof

A technology of dopamine and nanoparticles, applied in the direction of material electrochemical variables, etc., can solve the problems of high price and low detection limit

Inactive Publication Date: 2013-07-10
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The aqueous solution of platinum precursor reacts with reducing materials to form platinum nanoparticles. Platinum nanoparticles have the advantages of low detection limit and good stability, but they are expensive

Method used

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  • Dopamine polymer/noble metal nanoparticle electrochemical sensor as well as preparation method and application thereof
  • Dopamine polymer/noble metal nanoparticle electrochemical sensor as well as preparation method and application thereof
  • Dopamine polymer/noble metal nanoparticle electrochemical sensor as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Preparation and Characterization of Polydopamine / Silver Nanoparticles

[0032] The polydopamine / silver nanoparticle composite was prepared by layer-by-layer assembly method, and the preparation process is shown in the schematic diagram figure 1 . Dopamine solution can be prepared by dissolving dopamine in tris-HCl buffer solution with pH=8.5. 0.1mg / mL dopamine solution was dropped on ITO glass and carbon-coated copper grid respectively, and after drying, 2mg / mL silver nitrate solution was added dropwise, and so on, and multilayer silver nanoparticles could be obtained.

[0033] Using a field emission scanning electron microscope (FESEM, figure 2 ) to characterize the complex. Under aerobic and alkaline conditions, dopamine can easily form a polymer layer on the surface of ITO glass, and its surface is relatively smooth, such as figure 2 as shown in a. After the cleaned polydopamine surface is in contact with silver ions, the silver ions are reduced to silver n...

Embodiment 2

[0042] 1. Preparation of polydopamine / platinum nanoparticles electrochemical sensor

[0043] A glassy carbon electrode with a diameter of 3 mm was polished with sandpaper, Al 2 o 3 (particle size of 0.3 μm) after grinding and polishing, ultrasonically wash with double distilled water, ethanol and double distilled water in sequence. Dissolve 2 mg of dopamine in 20 mL of tris-HCl buffer solution to prepare a 0.1 mg / mL dopamine solution. Add 6 μL of newly prepared dopamine solution dropwise to the treated glassy carbon electrode, and let it dry naturally; then drop 6 μL of 2 mg / mL chloroplatinic acid solution onto the dry polydopamine-modified glassy carbon electrode, and drop repeatedly Plus, a multilayer platinum nanoparticle electrochemical sensor can be fabricated.

[0044] 2. Determination of Hydrogen Peroxide Using Platinum Nanoparticle Electrochemical Sensor

[0045] In 0.1mol / L PBS buffer solution with pH=7.0 or in the test solution containing hydrogen peroxide, use C...

Embodiment 3

[0047] 1. Preparation of poly-levodopa / silver nanoparticles electrochemical sensor

[0048] A glassy carbon electrode with a diameter of 3 mm was polished with sandpaper, Al 2 o 3 (particle size of 0.3 μm) after grinding and polishing, ultrasonically wash with double distilled water, ethanol and double distilled water in sequence. Dissolve 2 mg of levodopa in 20 mL of tris-HCl buffer solution to prepare a 0.1 mg / mL levodopa solution. Add 6 μL of newly prepared levodopa solution dropwise to the treated glassy carbon electrode and let it dry naturally; then drop 6 μL of 2 mg / mL silver nitrate solution onto the dry poly-levodopa-modified glassy carbon electrode , repeated dropwise, can make multi-layer silver nanoparticles electrochemical sensor.

[0049] 2. Determination of hydrogen peroxide using silver nanoparticles electrochemical sensor

[0050] In 0.1mol / L PBS buffer solution with pH=7.0 or in the test solution containing hydrogen peroxide, use CHI660c electrochemical a...

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Abstract

The invention provides a dopamine polymer / noble metal nanoparticle electrochemical sensor as well as a preparation method and application thereof. The novel bioelectrochemical sensor is a three-electrode system sensor, wherein a reference electrode is a saturated calomel electrode, a counter electrode is a platinum wire electrode, and a working electrode is a glassy-carbon electrode modified by noble metal nanoparticles. According to the invention, noble metal nanoparticles are prepared by dropping a new-made dopamine or a ramification solution thereof and a water solution of a noble metal precursor on the glassy-carbon electrode layer by layer and reacting through a self-assembly method; and a dopamine polymer generated through the reaction has adhesion capacity and can increase the firmness of the noble metal nanoparticles and the glassy-carbon electrode. The dopamine polymer / noble metal nanoparticle electrochemical sensor provided by the invention can be applied to the detection of hydrogen peroxide; and a result indicates that the dopamine polymer / noble metal nanoparticle electrochemical sensor provided by the invention has the advantages of simple and fast preparation method, high firmness, low detection limit, good reproducibility and fast response.

Description

technical field [0001] The invention provides a dopamine polymer / noble metal nanoparticle electrochemical sensor, a preparation method and application thereof. Background technique [0002] As a strong oxidant, hydrogen peroxide has bleaching and bactericidal effects, and is widely used in many fields such as clinical, environmental, food, pharmaceutical and biological research. However, as an air pollutant, hydrogen peroxide is one of the factors for the formation of acid rain; in addition, high concentrations of hydrogen peroxide damage the eyes and skin and affect human health. After the residual hydrogen peroxide in food and packaging materials enters the human body, it has great side effects. It can directly stimulate mucous membrane tissue. If it is used in excess, it will also damage human cells on a large scale, and release active oxygen free radicals through chemical reactions causing Human cells become cancerous, accelerate human aging, etc. Therefore, the rapid ...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 王丰韩睿刘桂亭陈惠芬余波文颖任天瑞王虎
Owner SHANGHAI NORMAL UNIVERSITY
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