The invention relates to the technical field of
biosynthesis, in particular to a preparation method of gamma-
aminobutyric acid. The invention provides a method for preparing gamma-
aminobutyric acid, according to the method, gamma-
aminobutyric acid is catalytically synthesized by using a
mutant of
glutamate decarboxylase screened by the inventor, the problems of low
catalytic efficiency, long conversion time and limited reuse times of
enzyme are solved, the
immobilized enzyme can be continuously subjected to at least 20 rounds of
enzyme catalytic reaction, and the yield of gamma-aminobutyric acid is increased. And moreover, the
catalytic efficiency is not obviously reduced, the substrate conversion rate can still be stabilized at 99% or above, and the method is suitable for industrial production.