37 results about "Hyaluronidase activity" patented technology

The invention provides a traditional Chinese medicine preparation for treating allergic rhinitis and a preparation method thereof, and relates to the technical field of traditional Chinese medicine pharmacy, the traditional Chinese medicine preparation is prepared from radix asteris, schizonepeta spike, cynanchum paniculatum, perillaseed, spina gleditsiae, cottonrose hibiscus leaf, magnolia flower, semen brassicae, cortex lycii radicis, dendrobe, oroxylum indicum and rhodomyrtus tomentosa; an in-vitro experiment shows that the traditional Chinese medicine preparation can remarkably inhibit the activity of hyaluronidase, also can inhibit the release of beta-HEX in an RBL-2H3 cell degranulation model, reduces the HIS level in a cell supernatant, and effectively improves the morphological abnormality caused by degranulation of RBL-2H3 cells; in-vivo experiments show that the traditional Chinese medicine preparation can significantly reduce behavioral scores of mice suffering from allergic rhinitis, inhibit expression of inflammatory factors TNF-alpha, serum Ig E and OVE-sIg E levels and HA concentration, reduce histopathologic changes caused by allergic rhinitis, effectively repair immune injuries of nasal mucosa of the mice and improve the curative effect of the mice. And a safe and effective new strategy is provided for the treatment of allergic rhinitis.

This invention relates to a hyaluronic acid composite hydrogel based on the supramolecular self-assembly of natural molecules and its preparation method, belonging to the field of gel formulation technology. The invention first involves the self-assembly of specific natural molecules or their derivatives possessing hyaluronidase inhibitory and anti-inflammatory activities into stable supramolecular self-assemblies through intermolecular non-covalent interactions. Subsequently, this supramolecular self-assembly is combined with hyaluronic acid through various interactions such as physical entanglement, hydrogen bonding, van der Waals forces, or appropriate chemical cross-linking to construct a composite hydrogel with a uniform internal structure and stable performance. This invention not only achieves long-term, controllable sustained release of natural active ingredients in the gel through supramolecular loading, continuously inhibiting hyaluronidase activity and local inflammatory responses, but also avoids the residual risks associated with high dosages of traditional chemical cross-linking agents, ultimately obtaining a high-quality product that meets the appearance and performance requirements of medical hyaluronic acid gels.

The embodiment of the invention discloses citrus peel pectin, citrus peel pectin emulsion and application. The content of the citrus peel pectin is 66-67%, the protein content is 13-14%, and the esterification degree is 72-73%; the sugar comprises the following components in percentage by mole: 58-59% of galacturonic acid, 25-26% of arabinose, 5-6% of galactose, 4-5% of rhamnose, 2-3% of glucose and 2-3% of xylose. The citrus peel pectin provided by the invention has excellent emulsibility; the citrus peel pectin emulsion has excellent emulsion stability, and shows a better encapsulation effect on LBE. The LBE emulsion obtained by encapsulating LBE by using CP has the characteristics of high encapsulation efficiency, low toxicity and high biocompatibility, and the synergistic effect of CP obviously enhances the antioxidant activity of LBE and the activity inhibition rate of tyrosinase, elastase and hyaluronidase, so that the LBE emulsion can be used as a novel anti-aging emulsion formula to be applied to cosmetics, and has a good application prospect. Good application prospects are realized.

The invention relates to the technical field of marine microorganisms, in particular to application of lactobacillus salivarius NHNK-603 in preparation of products for improving tooth color and gingivitis. The strain provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2024416. Experiments show; the NHNK-603 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

This invention provides a method for preparing safflower extract and its application. The extract is obtained by extracting safflower using a specific ratio of organic solution and subsequent post-processing. The extraction method of this invention is simple, requires a short production cycle for the raw materials, and the obtained extract has significant effects in inhibiting hyaluronidase activity, reducing transepidermal water loss, and suppressing sebum secretion. It can be used in moisturizing, soothing, oil-controlling, and repairing products.

The present application relates to the field of biotechnology, in particular to a kind of hyaluronidase binding cyclic peptide, the cyclic peptide is obtained by screening phage display technology, its structure includes X 1(n) CX3X4X5X6X7X8X9X 10 CX 12(m) It has the efficacy of inhibiting hyaluronidase activity, higher transdermal absorption capacity and / or anti-inflammatory, can be used as hyaluronidase (especially PH20) inhibitor, applied in the fields of medicine, food and cosmetics.

This invention relates to a Torreya grandis seed polypeptide with antioxidant and hyaluronidase inhibitory activities, its preparation, and its application. The method includes the following steps: S1, mixing Torreya grandis seed powder and ethanol for defatting to obtain a solid phase; S2, preparing the solid phase into a solution, acidifying it, then enzymatically hydrolyzing it with pepsin, inactivating the enzyme, centrifuging to collect the liquid phase, and freeze-drying it to obtain Torreya grandis seed polypeptide powder. Compared with the prior art, this invention provides a simple, reasonable, and efficient preparation method for Torreya grandis seed polypeptide, exhibiting good activity. It also proposes the application of Torreya grandis seed polypeptide in antioxidant and hyaluronidase activity inhibition, expanding its application in the fields of cosmeceuticals and food.

The invention relates to a preparation method of a purslane extracting solution with a soothing effect, and belongs to the field of cosmetics and biological extraction. The preparation method comprises the following steps: crushing fresh wild purslane, adding a butanediol-water mixed solvent, carrying out ultrasonic wall breaking treatment at 55-58 DEG C, and adding plant fiber hydrolase, vegetable gum hydrolase, plant starch hydrolase and plant protein hydrolase to carry out synergistic enzymolysis extraction; heating, inactivating and filtering the extracting solution, sequentially performing multi-stage purification by adopting chitosan flocculation, activated carbon decoloration, ceramic membrane filtration, ultrafiltration and nanofiltration, and collecting trapped fluid with the molecular weight of more than 150-300 Da through nanofiltration; and adding the humectant and the preservative. Through combination of ultrasonic-enzymolysis synergistic extraction and multi-stage membrane separation and purification, active ingredients such as polysaccharide and flavone in purslane are efficiently reserved under mild conditions, the inhibition rate of the obtained extracting solution to hyaluronidase activity reaches 20% or above, and the extracting solution has a soothing effect and good product stability and can be applied to soothing cosmetics or skin care products.

The invention discloses a hydrogel sensing material for hyaluronidase activity detection and a preparation method and application thereof, and belongs to the technical field of biosensors. The hydrogel sensing material for hyaluronidase activity detection comprises: a three-dimensional network structure formed by crosslinking hyaluronate and polyethyleneimine; methylene blue molecules are loaded in the three-dimensional network structure. Based on the amount of the raw materials, the mass ratio of the hyaluronate to the methylene blue is 24-60; the mass ratio of the hyaluronate to the polyethyleneimine is 2 to 5. According to the method for quantitatively detecting hyaluronidase by using the hydrogel sensing material for hyaluronidase activity detection, the activity concentration of hyaluronidase can be rapidly and sensitively detected, the hyaluronidase activity detection can be realized only in 8-15 minutes, the specificity is good, and the anti-interference performance is strong.

The present disclosure relates to the field of biotechnology, in particular to a method for detecting hyaluronidase activity and use thereof, and a kit and a method for using the same. The method and the kit have high sensitivity and good stability, and only a small amount of sample is required.

The invention relates to the technical field of cosmetics, in particular to a firming and relieving composition and application thereof.The skin care composition is prepared from peach resin extract, inonotus obliquus extract, purslane extract, sodium hyaluronate cross-linked polymer and porphyridium cruentum extract. The composition obtained by compounding the raw materials can significantly inhibit the activity of elastinase, has a certain inhibition effect on the activity of hyaluronidase, has significant tightening and soothing effects, and is mild and non-irritating.

The invention relates to the technical field of biology, in particular to lactobacillus paracasei and application of the lactobacillus paracasei to improvement of tooth color and gingivitis. The strain provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251304. Experiments show; the NHNK-632 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

The invention discloses a soothing and repairing skin care composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The skin care composition is prepared from the following components in parts by mass: 3 to 7 parts of herba centellae extract, 0.5 to 1 part of radix scutellariae extract, 2 to 4 parts of rhizoma polygoni cuspidati extract, 1 to 3 parts of radix gentianae extract and 15 to 20 parts of rhizoma atractylodis macrocephalae extract. Wherein the rhizoma atractylodis macrocephalae root extract is prepared by adopting a compound fermentation process of rhodotorula glutinis, lactobacillus brewer and lactobacillus paracasei, and active ingredients are richer. Through the synergistic effect of multiple components, the composition can significantly inhibit secretion of inflammatory factors IL-6 and activity of hyaluronidase and promote HaCaT cell migration, has excellent soothing, anti-inflammatory and repairing effects, and can effectively improve the problems of skin sensitivity, dryness and barrier injury.

The application discloses a traditional Chinese medicine composition for inhibiting hyaluronidase activity and a hyaluronidase inhibitor. The traditional Chinese medicine composition for inhibiting hyaluronidase activity comprises 1-12 parts of Mirabilis jalapa, 1-12 parts of Cnidium monnieri and 1-6 parts of Coptis chinensis by weight. The hyaluronidase inhibitor comprises 1-2 parts of Tween, 2-5 parts of lecithin, 0.1-0.5 parts of cholesterol, 40-80 parts of the traditional Chinese medicine composition and 80-100 parts of deionized water by weight. The traditional Chinese medicine composition and the hyaluronidase inhibitor can effectively inhibit the activity of hyaluronidase. The combination of Mirabilis jalapa, Cnidium monnieri and Coptis chinensis can effectively inhibit the activity of hyaluronidase. Mirabilis jalapa, Cnidium monnieri and Coptis chinensis have a remarkable synergistic effect on the activity of hyaluronidase.

The invention discloses application of a citric acid-based biological material as a cosmetic raw material, and belongs to the technical field of cosmetics, and the citric acid-based biological material can be a citric acid-based small molecule or a citric acid-based polymer; the citric acid-based biological material not only has excellent biocompatibility and chemical stability, but also can selectively absorb ultraviolet rays and release blue fluorescence through unique molecular design and structural modification by utilizing the characteristic that the citric acid-based biological material can effectively inhibit generation of melanin in skin, so that the whitening and sunscreen effects are enhanced. In addition, the citric acid-based biological material also shows an inhibiting effect on the activity of elastinase and hyaluronidase, and has the effects of tightening, resisting wrinkles, relieving and removing cutin. The invention provides novel molecular structure design and formula selection for various types of cosmetics for whitening, sunscreen and the like, and provides an innovative thought for efficient and safe application of related products.

The invention discloses hyaluronidase and application thereof in preparation of small-molecular sodium hyaluronate, and provides hyaluronidase, a recombinant strain is induced to express recombinant hyaluronidase, and after the hyaluronidase is separated and purified, the small-molecular sodium hyaluronate is specifically and efficiently prepared. The hyaluronidase disclosed by the invention is high in activity, rapid cutting of macromolecular sodium hyaluronate can be realized under the condition of 25 DEG C, the reaction time is short, the finally prepared micromolecular sodium hyaluronate is uniform in molecular weight, low in protein residue, bright white in color, free of obvious browning, high in process yield and low in cost.

This invention discloses an extracellular polysaccharide of *Pichia pastoris* ethanol, its preparation method, and its application, belonging to the field of bioengineering. The supernatant of *Pichia pastoris* Y49 fermentation broth is centrifuged, then subjected to ethanol precipitation, protein removal, dialysis, ion exchange column chromatography, and ultrafiltration, followed by lyophilization to obtain purified *Pichia pastoris* ethanol extracellular polysaccharide (PEEPS-1). This invention also discloses the structure of *Pichia pastoris* ethanol extracellular polysaccharide (PEEPS-1), which is an α-mannan composed solely of mannose, with a molecular weight of 7.11 × 10⁻⁶. 4 The main chain of Da, PEEPS-1 consists of (1→2)-mannose residues, with long side chains composed of (1→6)-mannose residues and (1→2)-mannose residues, and short side chains directly connected to the ends. The PEEPS-1 extracellular polysaccharide from *Pichia pastoris* prepared in this invention exhibits significant in vitro antioxidant activity, hygroscopic and moisturizing properties, and inhibition of hyaluronidase activity, making it suitable for use in cosmetics. This application provides a new approach for the development of *Pichia pastoris* extracellular polysaccharides.

This invention discloses a deer antler mushroom extract, its preparation method, and its application in promoting skin barrier repair, belonging to the field of biotechnology. The preparation method includes: extracting the fruiting bodies of deer antler mushrooms using ethanol solution as the extraction solvent and employing a progressively decreasing material-to-liquid ratio; combining the extracts to obtain a deer antler mushroom ethanol extract; extracting the residue of the deer antler mushroom ethanol extract using water as the extraction solvent and employing a progressively decreasing material-to-liquid ratio, and combining the extracts; subjecting the extracts to alcohol precipitation to obtain a deer antler mushroom water-extracted polysaccharide; and combining the deer antler mushroom ethanol extract and the deer antler mushroom water-extracted polysaccharide to obtain the deer antler mushroom extract. The deer antler mushroom extract prepared by this invention has a dual function: inhibiting hyaluronidase activity and repairing the skin barrier by promoting keratinocyte proliferation and migration and upregulating the expression of barrier-related genes. It has clear and broad application prospects in the fields of cosmetics and skin repair pharmaceuticals.

The present application provides a fluffy oil-control anti-dandruff composition, a preparation method and application thereof, and relates to the technical field of cosmetics. The fluffy oil-control anti-dandruff composition comprises a plant composition and an anti-dandruff composition, wherein the plant composition is composed of Capra hircus, Terminalia chebula, flower / leaf of Tamarix and Artemisia annua in a mass ratio of (1-20):20:1:10, has the effects of synergistically inhibiting Malassezia and inhibiting hyaluronidase activity; the anti-dandruff composition comprises at least one of piroctone olamine, white willow bark and salvia miltiorrhiza extract, and is used for enhancing the effects of inhibiting bacteria, controlling oil and relieving. The fluffy oil-control anti-dandruff composition can simultaneously regulate the microecological balance of the scalp, and improve the problems of dandruff, excessive oil and scalp itching caused by sensitive scalp, and can be widely used in the preparation process of products for improving dandruff, excessive oil and scalp itching caused by sensitive scalp.

The present invention discloses a multi-component synovitis therapeutic composition based on cartilage tissue repair and a use thereof, relating to the technical field of biomedicine. The composition comprises glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone, and graded molecular weight sodium hyaluronate. By compounding these components in precise parts by weight, the present invention constructs a gradient sustained-release and targeted delivery system for synovitis lesions. Dimethyl sulfone specifically inhibits hyaluronidase activity in the inflamed joint cavity, significantly reducing sodium hyaluronate degradation and prolonging the retention time of each active ingredient at the lesion site. High molecular weight sodium hyaluronate rapidly forms a lubricating protective film to achieve immediate analgesia and relieve ankylosis, while medium- and low-molecular-weight sodium hyaluronate penetrates the synovial barrier and carries cartilage repair components to the cartilage defect area, thereby achieving synergistic enhancement and comprehensively improving synovitis treatment efficacy.

The present application belongs to the field of microbial technology, and particularly relates to a strain of M. extremo-utricum and application thereof. The M. extremo-utricum of the present application is derived from Prinsepia uniflora Batal. in Haba Snow Mountain, Yunnan, and has a preservation number of GDMCC No: 65163. The fermentation filtrate of the M. extremo-utricum exhibits outstanding effects of scavenging DPPH free radicals, scavenging ABTS free radicals and inhibiting hyaluronidase activity, and has effects of antioxidation, soothing and anti-aging. Therefore, the M. extremo-utricum can be used as a new type of green and safe functional additive in the field of skin care products, and is added in make-up and personal care products. In addition, the operation of preparing the fermentation filtrate by using the M. extremo-utricum is simple, and the operation process is economical, pollution-free, safe, environmentally friendly, sustainable, and the devices used are easy to clean. These characteristics show that the technology is not only suitable for large-scale industrial production, but also has great potential in the development of skin care product raw materials.

This invention discloses a method for preparing an aqueous extract of Artemisia argyi and its application, particularly in the preparation of drugs for treating allergic contact dermatitis. The preparation method includes step 1: naturally air-drying harvested Artemisia argyi leaves, followed by mechanical pulverization to obtain Artemisia floss raw material; step 2: mixing the Artemisia floss raw material with pure water, soaking at room temperature, extracting with ultrasound assistance, combining the extracts, decocting in a water bath, concentrating under reduced pressure, and freeze-drying to obtain the Artemisia argyi aqueous extract. This invention provides an Artemisia argyi aqueous extract, its preparation method, and its application. This Artemisia argyi aqueous extract has significant anti-allergic and anti-irritant effects, effectively inhibiting hyaluronidase activity, reducing erythrocyte hemolysis rate, and exhibiting good therapeutic effects on 2,4-dinitrofluorobenzene-induced allergic contact dermatitis. It also has high bioavailability and good safety. This invention lays an important foundation for developing efficient and safe natural anti-allergic products.

The application belongs to the field of chemical industry, and particularly relates to a magnolia officinalis polysaccharide, a preparation method and application thereof, and comprises the following steps: S1: ultrasonic-assisted enzyme extraction of magnolia officinalis polysaccharide: mixing magnolia officinalis powder with cellulase, dissolving in water, and then performing ultrasonic extraction to obtain a magnolia officinalis polysaccharide extract liquid; S2: impurity removal of the magnolia officinalis polysaccharide extract liquid: centrifuging the magnolia officinalis polysaccharide extract liquid obtained in step S1, taking a filtrate, and sequentially performing TCA method protein removal, dialysis, ethanol precipitation and freeze-drying to obtain the magnolia officinalis polysaccharide. Compared with the prior art, the application solves the problems of complex magnolia officinalis polysaccharide preparation, high cost, limited application of the magnolia officinalis polysaccharide and the like in the prior art, realizes the preparation of the magnolia officinalis polysaccharide with simple preparation method, reasonable process, high extraction efficiency and good purification effect, and proposes the application of the magnolia officinalis polysaccharide in hyaluronidase activity inhibition and ultraviolet resistance, thereby expanding the application of the magnolia officinalis polysaccharide in the field of chemical industry.

The present invention relates to: a use of a subcutaneous injection formulation comprising hyaluronidase and used in combination with an antibody-drug conjugate; a subcutaneous injection kit comprising same; and a method for treating diseases including cancer using same. Preferably, the present invention relates to a method for treating diseases by subcutaneously injecting an antibody-drug conjugate, in which an antibody against a cancer-specific antigen and a drug having the characteristics of killing or inhibiting the growth of cancer cells are conjugated, together with an enzyme having hyaluronidase activity, and a subcutaneous administration kit, and presents an injection route that minimizes side effects, particularly systemic toxicity (e.g., neutrophil reduction) and local toxicity of drugs, through subcutaneous injection using hyaluronidase.

The invention belongs to the technical field of microorganisms, and in particular relates to a Merschhia pastoris strain and application thereof. The Metzithromyces rupestris provided by the invention is derived from prinsepia utilis royle in Harba snow mountain of Yunnan province, and the preservation number is GDMCC No: 65163. The fermentation filtrate shows outstanding effects of removing DPPH free radicals, removing ABTS free radicals, inhibiting hyaluronidase activity and the like, and has the effects of resisting oxidation, relieving and resisting aging. Therefore, the product can be used as a novel, green and safe functional additive in the field of skin care products, and can be added in beauty makeup and personal care products. In addition, the operation of preparing the fermentation filtrate by using the strain is simple and convenient, the operation process is economic, pollution-free, safe, environment-friendly and sustainable, the used devices are easy to clean, and the characteristics show that the technology is not only suitable for large-scale industrial production, but also has huge potential in the development of raw materials of skin care products.

Disclosed is an additive with anti-hyaluronidase activity comprising at least one positively charged amino acid and at least one hydrophobic amino acid, its use for counteracting the degradation of fillers or topical formulations based on hyaluronic acid and a method for inhibiting the degradation of injected hyaluronic acid for cosmetic purposes.

The invention belongs to the technical field of pharmacy, and particularly relates to a method for maintaining the activity of hyaluronidase in a composition. The invention provides a method for maintaining stability of hyaluronidase. The method can eliminate potential safety hazards caused by instability of hyaluronidase in the process of assisting antibiotic administration with hyaluronidase, promote rapid absorption of antibiotics after administration, effectively improve anaphylactic reaction and remarkably improve patient compliance.

The present application belongs to the technical field of genetic engineering, and particularly relates to a Streptococcus agalactiae Mu-20 and a construction method and application thereof. The present application constructs a fish-derived Streptococcus agalactiae strain with a capsule polysaccharide deficiency, no hyaluronidase activity, no hemolysis and no trehalose fermentation, and the strain number is Mu-20. The strain was preserved in the Guangdong Microbial Culture Collection Center on June 13, 2022, and the preservation number is GDMCC No: 62540. The strain does not carry a chloramphenicol resistance marker gene, has high biological safety, has no risk of virulence returning to strength, has high immunogenicity, can protect immunized fish against the invasion of Streptococcus agalactiae, and is easy to cultivate, simple to use and low in production cost. The strain involved in the present application can be used to prepare a live attenuated vaccine for fish streptococcosis, and has extremely high commercial application value.

The invention discloses a multi-dimensional oil-control soothing composition and a preparation method thereof, and relates to the technical field of cosmetics. The multi-dimensional oil-controlling and soothing composition is prepared from a composite plant extract, dipeptide-15, capryloyl glycine, EDTA-2Na, sodium hydroxide and a solvent, and the composite plant extract is obtained by taking artemisia capillaris and oldenlandia diffusa as raw materials according to a specific mass ratio and extracting by adopting a dynamic countercurrent extraction technology. The obtained composite plant extract has a remarkable effect of inhibiting propionibacterium acnes; the obtained composite plant extract, dipeptide-15 and capryloyl glycine are combined according to a specific mass percentage to be used in the multi-dimensional oil-controlling and soothing composition, the activity of 5 alpha-reductase and the activity of hyaluronidase can be remarkably inhibited, and the multi-dimensional oil-controlling and soothing composition has the remarkable oil-controlling and soothing effects and is mild and non-irritant.

The invention provides a preparation method and application of flos carthami extract. The flos carthami extract is obtained by performing extraction and post-treatment on flos carthami by using an organic solution with a specific ratio. The extraction method disclosed by the invention is simple, the production cycle of the required raw materials is short, and the obtained extract has remarkable effects of inhibiting hyaluronidase activity, reducing percutaneous moisture loss and inhibiting grease secretion, and can be applied to products for moisturizing, relieving, controlling oil and repairing.