52 results about "Hyaluronidase activity" patented technology

The invention discloses a molecular modification design method for improving the catalytic activity and the thermal stability of citrobacter vinegaticus-derived hyaluronidase and expression application of the citrobacter vinegaticus-derived hyaluronidase. The interaction of citrobacter vinegaticus hyaluronidase active pocket amino acid residues and hyaluronate tetrasaccharide molecules is visually explored through Pymol, conservative analysis of pocket amino acid evolution is carried out through Consurf, and rational design is carried out through policies of FoldX for calculating enzyme-substrate binding free energy, so that the hyaluronidase catalytic activity is improved. A mutant with significantly improved enzyme activity is obtained through screening, and the thermal stability of the enzyme is further improved through PROSS design and an analysis strategy of mutation site amino acid evolution conservative property and position. According to the invention, the sequence, structure and function of hyaluronidase are deeply studied, and hyaluronidase is mutated from two aspects of stabilizing a catalytic structure and promoting a catalytic reaction, so that a high-activity enzyme with higher application value in industrial production is obtained.

The invention provides a traditional Chinese medicine preparation for treating allergic rhinitis and a preparation method thereof, and relates to the technical field of traditional Chinese medicine pharmacy, the traditional Chinese medicine preparation is prepared from radix asteris, schizonepeta spike, cynanchum paniculatum, perillaseed, spina gleditsiae, cottonrose hibiscus leaf, magnolia flower, semen brassicae, cortex lycii radicis, dendrobe, oroxylum indicum and rhodomyrtus tomentosa; an in-vitro experiment shows that the traditional Chinese medicine preparation can remarkably inhibit the activity of hyaluronidase, also can inhibit the release of beta-HEX in an RBL-2H3 cell degranulation model, reduces the HIS level in a cell supernatant, and effectively improves the morphological abnormality caused by degranulation of RBL-2H3 cells; in-vivo experiments show that the traditional Chinese medicine preparation can significantly reduce behavioral scores of mice suffering from allergic rhinitis, inhibit expression of inflammatory factors TNF-alpha, serum Ig E and OVE-sIg E levels and HA concentration, reduce histopathologic changes caused by allergic rhinitis, effectively repair immune injuries of nasal mucosa of the mice and improve the curative effect of the mice. And a safe and effective new strategy is provided for the treatment of allergic rhinitis.

The invention discloses application of fermented lactobacillus mucus in preparation of a product for improving tooth color and gingivitis, and relates to the technical field of microorganisms. The lactobacillus fermentum NHNK-623 disclosed by the invention is preserved in the China Center for Type Culture Collection on November 18, 2024, and the preservation number of the lactobacillus fermentum NHNK-623 is CCTCC (China Center for Type Culture Collection) NO: M 20242574. The NHNK-623 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

This invention relates to a hyaluronic acid composite hydrogel based on the supramolecular self-assembly of natural molecules and its preparation method, belonging to the field of gel formulation technology. The invention first involves the self-assembly of specific natural molecules or their derivatives possessing hyaluronidase inhibitory and anti-inflammatory activities into stable supramolecular self-assemblies through intermolecular non-covalent interactions. Subsequently, this supramolecular self-assembly is combined with hyaluronic acid through various interactions such as physical entanglement, hydrogen bonding, van der Waals forces, or appropriate chemical cross-linking to construct a composite hydrogel with a uniform internal structure and stable performance. This invention not only achieves long-term, controllable sustained release of natural active ingredients in the gel through supramolecular loading, continuously inhibiting hyaluronidase activity and local inflammatory responses, but also avoids the residual risks associated with high dosages of traditional chemical cross-linking agents, ultimately obtaining a high-quality product that meets the appearance and performance requirements of medical hyaluronic acid gels.

The embodiment of the invention discloses citrus peel pectin, citrus peel pectin emulsion and application. The content of the citrus peel pectin is 66-67%, the protein content is 13-14%, and the esterification degree is 72-73%; the sugar comprises the following components in percentage by mole: 58-59% of galacturonic acid, 25-26% of arabinose, 5-6% of galactose, 4-5% of rhamnose, 2-3% of glucose and 2-3% of xylose. The citrus peel pectin provided by the invention has excellent emulsibility; the citrus peel pectin emulsion has excellent emulsion stability, and shows a better encapsulation effect on LBE. The LBE emulsion obtained by encapsulating LBE by using CP has the characteristics of high encapsulation efficiency, low toxicity and high biocompatibility, and the synergistic effect of CP obviously enhances the antioxidant activity of LBE and the activity inhibition rate of tyrosinase, elastase and hyaluronidase, so that the LBE emulsion can be used as a novel anti-aging emulsion formula to be applied to cosmetics, and has a good application prospect. Good application prospects are realized.

The invention relates to the technical field of marine microorganisms, in particular to application of lactobacillus salivarius NHNK-603 in preparation of products for improving tooth color and gingivitis. The strain provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2024416. Experiments show; the NHNK-603 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

This invention provides a method for preparing safflower extract and its application. The extract is obtained by extracting safflower using a specific ratio of organic solution and subsequent post-processing. The extraction method of this invention is simple, requires a short production cycle for the raw materials, and the obtained extract has significant effects in inhibiting hyaluronidase activity, reducing transepidermal water loss, and suppressing sebum secretion. It can be used in moisturizing, soothing, oil-controlling, and repairing products.

The present application relates to the field of biotechnology, in particular to a kind of hyaluronidase binding cyclic peptide, the cyclic peptide is obtained by screening phage display technology, its structure includes X 1(n) CX3X4X5X6X7X8X9X 10 CX 12(m) It has the efficacy of inhibiting hyaluronidase activity, higher transdermal absorption capacity and / or anti-inflammatory, can be used as hyaluronidase (especially PH20) inhibitor, applied in the fields of medicine, food and cosmetics.

This invention relates to a Torreya grandis seed polypeptide with antioxidant and hyaluronidase inhibitory activities, its preparation, and its application. The method includes the following steps: S1, mixing Torreya grandis seed powder and ethanol for defatting to obtain a solid phase; S2, preparing the solid phase into a solution, acidifying it, then enzymatically hydrolyzing it with pepsin, inactivating the enzyme, centrifuging to collect the liquid phase, and freeze-drying it to obtain Torreya grandis seed polypeptide powder. Compared with the prior art, this invention provides a simple, reasonable, and efficient preparation method for Torreya grandis seed polypeptide, exhibiting good activity. It also proposes the application of Torreya grandis seed polypeptide in antioxidant and hyaluronidase activity inhibition, expanding its application in the fields of cosmeceuticals and food.

The invention relates to a preparation method of a purslane extracting solution with a soothing effect, and belongs to the field of cosmetics and biological extraction. The preparation method comprises the following steps: crushing fresh wild purslane, adding a butanediol-water mixed solvent, carrying out ultrasonic wall breaking treatment at 55-58 DEG C, and adding plant fiber hydrolase, vegetable gum hydrolase, plant starch hydrolase and plant protein hydrolase to carry out synergistic enzymolysis extraction; heating, inactivating and filtering the extracting solution, sequentially performing multi-stage purification by adopting chitosan flocculation, activated carbon decoloration, ceramic membrane filtration, ultrafiltration and nanofiltration, and collecting trapped fluid with the molecular weight of more than 150-300 Da through nanofiltration; and adding the humectant and the preservative. Through combination of ultrasonic-enzymolysis synergistic extraction and multi-stage membrane separation and purification, active ingredients such as polysaccharide and flavone in purslane are efficiently reserved under mild conditions, the inhibition rate of the obtained extracting solution to hyaluronidase activity reaches 20% or above, and the extracting solution has a soothing effect and good product stability and can be applied to soothing cosmetics or skin care products.

The invention discloses a hydrogel sensing material for hyaluronidase activity detection and a preparation method and application thereof, and belongs to the technical field of biosensors. The hydrogel sensing material for hyaluronidase activity detection comprises: a three-dimensional network structure formed by crosslinking hyaluronate and polyethyleneimine; methylene blue molecules are loaded in the three-dimensional network structure. Based on the amount of the raw materials, the mass ratio of the hyaluronate to the methylene blue is 24-60; the mass ratio of the hyaluronate to the polyethyleneimine is 2 to 5. According to the method for quantitatively detecting hyaluronidase by using the hydrogel sensing material for hyaluronidase activity detection, the activity concentration of hyaluronidase can be rapidly and sensitively detected, the hyaluronidase activity detection can be realized only in 8-15 minutes, the specificity is good, and the anti-interference performance is strong.

The invention relates to a lactobacillus rhamnosus LRa19 capable of inhibiting hyaluronidase activity and tryptase release and application of the lactobacillus rhamnosus LRa19. The lactobacillus rhamnosus LRa19 is classified and named as lactobacillus rhamnosus, the preservation number of the lactobacillus rhamnosus LRa19 is CGMCC (China General Microbiological Culture Collection Center) No.33767, and the preservation date of the lactobacillus rhamnosus LRa19 is March 10, 2025. The strain has good tolerance in gastric juice and cholate, and has the potential of smoothly entering the gastrointestinal tract of a human body. The strain can inhibit hyaluronidase activity and tryptase release, adjust Th1 / Th2 imbalance, improve the abundance of effective microbial communities in intestinal tracts, and effectively relieve and improve atopic dermatitis symptoms.

The present disclosure relates to the field of biotechnology, in particular to a method for detecting hyaluronidase activity and use thereof, and a kit and a method for using the same. The method and the kit have high sensitivity and good stability, and only a small amount of sample is required.

The invention relates to the technical field of cosmetics, in particular to a firming and relieving composition and application thereof.The skin care composition is prepared from peach resin extract, inonotus obliquus extract, purslane extract, sodium hyaluronate cross-linked polymer and porphyridium cruentum extract. The composition obtained by compounding the raw materials can significantly inhibit the activity of elastinase, has a certain inhibition effect on the activity of hyaluronidase, has significant tightening and soothing effects, and is mild and non-irritating.

The invention relates to the technical field of biology, in particular to lactobacillus paracasei and application of the lactobacillus paracasei to improvement of tooth color and gingivitis. The strain provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251304. Experiments show; the NHNK-632 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

The invention discloses a soothing and repairing skin care composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The skin care composition is prepared from the following components in parts by mass: 3 to 7 parts of herba centellae extract, 0.5 to 1 part of radix scutellariae extract, 2 to 4 parts of rhizoma polygoni cuspidati extract, 1 to 3 parts of radix gentianae extract and 15 to 20 parts of rhizoma atractylodis macrocephalae extract. Wherein the rhizoma atractylodis macrocephalae root extract is prepared by adopting a compound fermentation process of rhodotorula glutinis, lactobacillus brewer and lactobacillus paracasei, and active ingredients are richer. Through the synergistic effect of multiple components, the composition can significantly inhibit secretion of inflammatory factors IL-6 and activity of hyaluronidase and promote HaCaT cell migration, has excellent soothing, anti-inflammatory and repairing effects, and can effectively improve the problems of skin sensitivity, dryness and barrier injury.

The application discloses a traditional Chinese medicine composition for inhibiting hyaluronidase activity and a hyaluronidase inhibitor. The traditional Chinese medicine composition for inhibiting hyaluronidase activity comprises 1-12 parts of Mirabilis jalapa, 1-12 parts of Cnidium monnieri and 1-6 parts of Coptis chinensis by weight. The hyaluronidase inhibitor comprises 1-2 parts of Tween, 2-5 parts of lecithin, 0.1-0.5 parts of cholesterol, 40-80 parts of the traditional Chinese medicine composition and 80-100 parts of deionized water by weight. The traditional Chinese medicine composition and the hyaluronidase inhibitor can effectively inhibit the activity of hyaluronidase. The combination of Mirabilis jalapa, Cnidium monnieri and Coptis chinensis can effectively inhibit the activity of hyaluronidase. Mirabilis jalapa, Cnidium monnieri and Coptis chinensis have a remarkable synergistic effect on the activity of hyaluronidase.

Disclosed are human hyaluronidase 1 (HYAL1) mutants that highly express and exhibit hyaluronidase activity between pH 3.5 and pH 5.5.

The invention discloses application of a citric acid-based biological material as a cosmetic raw material, and belongs to the technical field of cosmetics, and the citric acid-based biological material can be a citric acid-based small molecule or a citric acid-based polymer; the citric acid-based biological material not only has excellent biocompatibility and chemical stability, but also can selectively absorb ultraviolet rays and release blue fluorescence through unique molecular design and structural modification by utilizing the characteristic that the citric acid-based biological material can effectively inhibit generation of melanin in skin, so that the whitening and sunscreen effects are enhanced. In addition, the citric acid-based biological material also shows an inhibiting effect on the activity of elastinase and hyaluronidase, and has the effects of tightening, resisting wrinkles, relieving and removing cutin. The invention provides novel molecular structure design and formula selection for various types of cosmetics for whitening, sunscreen and the like, and provides an innovative thought for efficient and safe application of related products.

The invention belongs to the technical field of skin care, and discloses a soothing, firming and anti-wrinkle composition, application and gel for fading cheilogramma The soothing, firming and anti-wrinkle composition is prepared from, by weight, 5-15 parts of hydrogenated polyisobutene, 0.1-1 part of citron fruit extract and 0.1-3 parts of peony seed oil. The hydrogenated polyisobutene, the citron fruit extract and the peony seed oil are compounded, so that the hyaluronidase activity inhibition rate and the DPPH free radical scavenging rate of the composition are improved, and the effects of soothing the skin, tightening the skin and resisting wrinkles can be achieved.

The invention discloses hyaluronidase and application thereof in preparation of small-molecular sodium hyaluronate, and provides hyaluronidase, a recombinant strain is induced to express recombinant hyaluronidase, and after the hyaluronidase is separated and purified, the small-molecular sodium hyaluronate is specifically and efficiently prepared. The hyaluronidase disclosed by the invention is high in activity, rapid cutting of macromolecular sodium hyaluronate can be realized under the condition of 25 DEG C, the reaction time is short, the finally prepared micromolecular sodium hyaluronate is uniform in molecular weight, low in protein residue, bright white in color, free of obvious browning, high in process yield and low in cost.

This invention discloses an extracellular polysaccharide of *Pichia pastoris* ethanol, its preparation method, and its application, belonging to the field of bioengineering. The supernatant of *Pichia pastoris* Y49 fermentation broth is centrifuged, then subjected to ethanol precipitation, protein removal, dialysis, ion exchange column chromatography, and ultrafiltration, followed by lyophilization to obtain purified *Pichia pastoris* ethanol extracellular polysaccharide (PEEPS-1). This invention also discloses the structure of *Pichia pastoris* ethanol extracellular polysaccharide (PEEPS-1), which is an α-mannan composed solely of mannose, with a molecular weight of 7.11 × 10⁻⁶. 4 The main chain of Da, PEEPS-1 consists of (1→2)-mannose residues, with long side chains composed of (1→6)-mannose residues and (1→2)-mannose residues, and short side chains directly connected to the ends. The PEEPS-1 extracellular polysaccharide from *Pichia pastoris* prepared in this invention exhibits significant in vitro antioxidant activity, hygroscopic and moisturizing properties, and inhibition of hyaluronidase activity, making it suitable for use in cosmetics. This application provides a new approach for the development of *Pichia pastoris* extracellular polysaccharides.

The invention discloses a traditional Chinese medicine composition with relieving and anti-allergy effects as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from a radix sophorae flavescentis extract, a cortex phellodendri extract and a pithecellobium clypearia extract in a mass ratio of (2-4): (1-2): (0.5-1). The composition can remarkably inhibit hyaluronidase activity, the inhibition rate reaches 67.68% or above, skin itch reaction induced by Chinese yam juice can be rapidly relieved within 5.5 min, the itching relieving aging of the composition is shortened by 26.5% compared with that of a positive control group, and therefore the effects of rapid itching relieving and strong relieving are achieved through the multi-target-point synergistic effect, and the effect of the composition is remarkably superior to that of a single component or a combination of every two components. A skin irritation experiment verifies that the composition has no sensitization risk. The technical problems that an existing chemical anti-allergy agent is large in irritation and single in effect of natural components are solved, the anti-allergy composition can be widely applied to cosmetic dosage forms such as cream and essence, and an innovative solution is provided for developing safe and efficient natural anti-allergy products.

This invention discloses a deer antler mushroom extract, its preparation method, and its application in promoting skin barrier repair, belonging to the field of biotechnology. The preparation method includes: extracting the fruiting bodies of deer antler mushrooms using ethanol solution as the extraction solvent and employing a progressively decreasing material-to-liquid ratio; combining the extracts to obtain a deer antler mushroom ethanol extract; extracting the residue of the deer antler mushroom ethanol extract using water as the extraction solvent and employing a progressively decreasing material-to-liquid ratio, and combining the extracts; subjecting the extracts to alcohol precipitation to obtain a deer antler mushroom water-extracted polysaccharide; and combining the deer antler mushroom ethanol extract and the deer antler mushroom water-extracted polysaccharide to obtain the deer antler mushroom extract. The deer antler mushroom extract prepared by this invention has a dual function: inhibiting hyaluronidase activity and repairing the skin barrier by promoting keratinocyte proliferation and migration and upregulating the expression of barrier-related genes. It has clear and broad application prospects in the fields of cosmetics and skin repair pharmaceuticals.

The invention belongs to the technical field of gene engineering, and particularly relates to streptococcus agalactiae Mu-20 and a construction method and application thereof. The fish-derived streptococcus agalactiae which is lack of capsular polysaccharide, free of hyaluronidase activity, free of hemolysis and negative in trehalose fermentation is constructed, the strain number is Mu-20, the fish-derived streptococcus agalactiae is preserved in Guangdong Microbial Culture Collection Center on June 13, 2022, and the preservation number is GDMCC No: 62540; the strain does not carry chloramphenicol resistance marker genes, is high in biological safety, has no risk of virulence enhancement, is high in immunogenicity, and can protect immunized fish from resisting invasion of streptococcus agalactiae, and meanwhile, the attenuated live vaccine is easy to culture, simple to use and low in production cost; the strain provided by the invention can be used for preparing attenuated live vaccines for fish streptococcosis, and has extremely high commercial application value.

The invention belongs to the technical field of probiotics, and particularly relates to bifidobacterium animalis XY01 with an inflammation relieving effect and application of the bifidobacterium animalis XY01. A novel Bifidobacterium animalis XY01 strain is separated from faeces of a healthy infant in Guangzhou city, Guangdong province, and research finds that the strain can inhibit aggregation of in-vivo macrophages at an inflammation part, promote subtraction of the macrophages from the inflammation part, reduce expression of in-vivo IL-6, IL-1beta and TNF-alpha, reduce hyaluronidase activity and inhibit inflammation of the infant. The chitinase activity is improved, and the potential inflammation relieving effect is achieved. Therefore, the bacterium has huge potential in the aspects of treatment and prevention of inflammation-related diseases, can be used for developing novel anti-inflammatory drugs, can also be used as a functional component to be added into health-care products, is beneficial to relieving inflammatory response and maintaining human health, and provides a new way and method for improving inflammation-related health problems.

The present invention discloses a traditional Chinese medicine composition with soothing and anti-allergic effects, as well as its preparation method and application. The composition comprises a Sophora flavescens extract, a Phellodendron amurense extract, and a Monkey Ear Ring extract in a mass ratio of (2-4):(1-2):(0.5-1). The composition significantly inhibits hyaluronidase activity, with an inhibition rate exceeding 67.68%. It can also rapidly relieve yam juice-induced skin pruritus within 5.5 minutes, with an antipruritic effect duration 26.5% shorter than that of the positive control group. This composition achieves a "fast antipruritic and strong soothing" effect through multi-target synergistic action, and its efficacy is significantly superior to that of single components and two-component combinations. Skin irritation experiments have verified that the composition has no allergic risk. The present invention solves the technical problems of existing chemical anti-allergic agents with high irritation and natural ingredients with limited efficacy. It can be widely used in cosmetic dosage forms such as creams and essences, providing an innovative solution for the development of safe and effective natural anti-allergic products.

The present application provides a fluffy oil-control anti-dandruff composition, a preparation method and application thereof, and relates to the technical field of cosmetics. The fluffy oil-control anti-dandruff composition comprises a plant composition and an anti-dandruff composition, wherein the plant composition is composed of Capra hircus, Terminalia chebula, flower / leaf of Tamarix and Artemisia annua in a mass ratio of (1-20):20:1:10, has the effects of synergistically inhibiting Malassezia and inhibiting hyaluronidase activity; the anti-dandruff composition comprises at least one of piroctone olamine, white willow bark and salvia miltiorrhiza extract, and is used for enhancing the effects of inhibiting bacteria, controlling oil and relieving. The fluffy oil-control anti-dandruff composition can simultaneously regulate the microecological balance of the scalp, and improve the problems of dandruff, excessive oil and scalp itching caused by sensitive scalp, and can be widely used in the preparation process of products for improving dandruff, excessive oil and scalp itching caused by sensitive scalp.

The invention discloses recombinant corynebacterium glutamicum with hyaluronic acid hydrolase displayed on the surface as well as a construction method and application of the recombinant corynebacterium glutamicum. The recombinant corynebacterium glutamicum with hyaluronic acid hydrolytic activity on the surface is obtained by fusing a hyaluronic acid hydrolase gene with genes of different anchoring proteins by adopting a surface display technology and introducing the fused gene into the corynebacterium glutamicum to carry out surface display, so that the recombinant corynebacterium glutamicum with hyaluronic acid hydrolytic activity on the surface is obtained. The recombinant corynebacterium glutamicum with ultrahigh hyaluronidase activity is obtained through optimization of anchoring protein, connecting peptide, culture conditions and the like, the strain can realize efficient immobilization and reuse of hyaluronidase, meanwhile, the redundant step of enzyme purification is omitted, the production cycle is shortened, and the production cost is reduced. Good application prospects are realized in the fields of cosmetics, medicines and the like.

The present invention discloses a multi-component synovitis therapeutic composition based on cartilage tissue repair and a use thereof, relating to the technical field of biomedicine. The composition comprises glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone, and graded molecular weight sodium hyaluronate. By compounding these components in precise parts by weight, the present invention constructs a gradient sustained-release and targeted delivery system for synovitis lesions. Dimethyl sulfone specifically inhibits hyaluronidase activity in the inflamed joint cavity, significantly reducing sodium hyaluronate degradation and prolonging the retention time of each active ingredient at the lesion site. High molecular weight sodium hyaluronate rapidly forms a lubricating protective film to achieve immediate analgesia and relieve ankylosis, while medium- and low-molecular-weight sodium hyaluronate penetrates the synovial barrier and carries cartilage repair components to the cartilage defect area, thereby achieving synergistic enhancement and comprehensively improving synovitis treatment efficacy.