11 results about "Hyaluronidase activity" patented technology

This invention relates to a hyaluronic acid composite hydrogel based on the supramolecular self-assembly of natural molecules and its preparation method, belonging to the field of gel formulation technology. The invention first involves the self-assembly of specific natural molecules or their derivatives possessing hyaluronidase inhibitory and anti-inflammatory activities into stable supramolecular self-assemblies through intermolecular non-covalent interactions. Subsequently, this supramolecular self-assembly is combined with hyaluronic acid through various interactions such as physical entanglement, hydrogen bonding, van der Waals forces, or appropriate chemical cross-linking to construct a composite hydrogel with a uniform internal structure and stable performance. This invention not only achieves long-term, controllable sustained release of natural active ingredients in the gel through supramolecular loading, continuously inhibiting hyaluronidase activity and local inflammatory responses, but also avoids the residual risks associated with high dosages of traditional chemical cross-linking agents, ultimately obtaining a high-quality product that meets the appearance and performance requirements of medical hyaluronic acid gels.

This invention provides a method for preparing safflower extract and its application. The extract is obtained by extracting safflower using a specific ratio of organic solution and subsequent post-processing. The extraction method of this invention is simple, requires a short production cycle for the raw materials, and the obtained extract has significant effects in inhibiting hyaluronidase activity, reducing transepidermal water loss, and suppressing sebum secretion. It can be used in moisturizing, soothing, oil-controlling, and repairing products.

The present application relates to the field of biotechnology, in particular to a kind of hyaluronidase binding cyclic peptide, the cyclic peptide is obtained by screening phage display technology, its structure includes X 1(n) CX3X4X5X6X7X8X9X 10 CX 12(m) It has the efficacy of inhibiting hyaluronidase activity, higher transdermal absorption capacity and / or anti-inflammatory, can be used as hyaluronidase (especially PH20) inhibitor, applied in the fields of medicine, food and cosmetics.

This invention relates to a Torreya grandis seed polypeptide with antioxidant and hyaluronidase inhibitory activities, its preparation, and its application. The method includes the following steps: S1, mixing Torreya grandis seed powder and ethanol for defatting to obtain a solid phase; S2, preparing the solid phase into a solution, acidifying it, then enzymatically hydrolyzing it with pepsin, inactivating the enzyme, centrifuging to collect the liquid phase, and freeze-drying it to obtain Torreya grandis seed polypeptide powder. Compared with the prior art, this invention provides a simple, reasonable, and efficient preparation method for Torreya grandis seed polypeptide, exhibiting good activity. It also proposes the application of Torreya grandis seed polypeptide in antioxidant and hyaluronidase activity inhibition, expanding its application in the fields of cosmeceuticals and food.

The present disclosure relates to the field of biotechnology, in particular to a method for detecting hyaluronidase activity and use thereof, and a kit and a method for using the same. The method and the kit have high sensitivity and good stability, and only a small amount of sample is required.

This invention discloses an extracellular polysaccharide of *Pichia pastoris* ethanol, its preparation method, and its application, belonging to the field of bioengineering. The supernatant of *Pichia pastoris* Y49 fermentation broth is centrifuged, then subjected to ethanol precipitation, protein removal, dialysis, ion exchange column chromatography, and ultrafiltration, followed by lyophilization to obtain purified *Pichia pastoris* ethanol extracellular polysaccharide (PEEPS-1). This invention also discloses the structure of *Pichia pastoris* ethanol extracellular polysaccharide (PEEPS-1), which is an α-mannan composed solely of mannose, with a molecular weight of 7.11 × 10⁻⁶. 4 The main chain of Da, PEEPS-1 consists of (1→2)-mannose residues, with long side chains composed of (1→6)-mannose residues and (1→2)-mannose residues, and short side chains directly connected to the ends. The PEEPS-1 extracellular polysaccharide from *Pichia pastoris* prepared in this invention exhibits significant in vitro antioxidant activity, hygroscopic and moisturizing properties, and inhibition of hyaluronidase activity, making it suitable for use in cosmetics. This application provides a new approach for the development of *Pichia pastoris* extracellular polysaccharides.

This invention discloses a deer antler mushroom extract, its preparation method, and its application in promoting skin barrier repair, belonging to the field of biotechnology. The preparation method includes: extracting the fruiting bodies of deer antler mushrooms using ethanol solution as the extraction solvent and employing a progressively decreasing material-to-liquid ratio; combining the extracts to obtain a deer antler mushroom ethanol extract; extracting the residue of the deer antler mushroom ethanol extract using water as the extraction solvent and employing a progressively decreasing material-to-liquid ratio, and combining the extracts; subjecting the extracts to alcohol precipitation to obtain a deer antler mushroom water-extracted polysaccharide; and combining the deer antler mushroom ethanol extract and the deer antler mushroom water-extracted polysaccharide to obtain the deer antler mushroom extract. The deer antler mushroom extract prepared by this invention has a dual function: inhibiting hyaluronidase activity and repairing the skin barrier by promoting keratinocyte proliferation and migration and upregulating the expression of barrier-related genes. It has clear and broad application prospects in the fields of cosmetics and skin repair pharmaceuticals.

The present invention discloses a multi-component synovitis therapeutic composition based on cartilage tissue repair and a use thereof, relating to the technical field of biomedicine. The composition comprises glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone, and graded molecular weight sodium hyaluronate. By compounding these components in precise parts by weight, the present invention constructs a gradient sustained-release and targeted delivery system for synovitis lesions. Dimethyl sulfone specifically inhibits hyaluronidase activity in the inflamed joint cavity, significantly reducing sodium hyaluronate degradation and prolonging the retention time of each active ingredient at the lesion site. High molecular weight sodium hyaluronate rapidly forms a lubricating protective film to achieve immediate analgesia and relieve ankylosis, while medium- and low-molecular-weight sodium hyaluronate penetrates the synovial barrier and carries cartilage repair components to the cartilage defect area, thereby achieving synergistic enhancement and comprehensively improving synovitis treatment efficacy.

The present application belongs to the field of microbial technology, and particularly relates to a strain of M. extremo-utricum and application thereof. The M. extremo-utricum of the present application is derived from Prinsepia uniflora Batal. in Haba Snow Mountain, Yunnan, and has a preservation number of GDMCC No: 65163. The fermentation filtrate of the M. extremo-utricum exhibits outstanding effects of scavenging DPPH free radicals, scavenging ABTS free radicals and inhibiting hyaluronidase activity, and has effects of antioxidation, soothing and anti-aging. Therefore, the M. extremo-utricum can be used as a new type of green and safe functional additive in the field of skin care products, and is added in make-up and personal care products. In addition, the operation of preparing the fermentation filtrate by using the M. extremo-utricum is simple, and the operation process is economical, pollution-free, safe, environmentally friendly, sustainable, and the devices used are easy to clean. These characteristics show that the technology is not only suitable for large-scale industrial production, but also has great potential in the development of skin care product raw materials.

This invention discloses a method for preparing an aqueous extract of Artemisia argyi and its application, particularly in the preparation of drugs for treating allergic contact dermatitis. The preparation method includes step 1: naturally air-drying harvested Artemisia argyi leaves, followed by mechanical pulverization to obtain Artemisia floss raw material; step 2: mixing the Artemisia floss raw material with pure water, soaking at room temperature, extracting with ultrasound assistance, combining the extracts, decocting in a water bath, concentrating under reduced pressure, and freeze-drying to obtain the Artemisia argyi aqueous extract. This invention provides an Artemisia argyi aqueous extract, its preparation method, and its application. This Artemisia argyi aqueous extract has significant anti-allergic and anti-irritant effects, effectively inhibiting hyaluronidase activity, reducing erythrocyte hemolysis rate, and exhibiting good therapeutic effects on 2,4-dinitrofluorobenzene-induced allergic contact dermatitis. It also has high bioavailability and good safety. This invention lays an important foundation for developing efficient and safe natural anti-allergic products.

This application relates to the field of cosmetic formulation technology, and particularly to a method for preparing modified hydroxytyrosol, its application, and cosmetics. The preparation method includes the following steps: hydroxytyrosol, collagen, alkaline protease, and arginine are reacted in an oxygen-containing environment in an aqueous solvent with a pH of 9-10 under stirring conditions to prepare a reaction product containing the modified hydroxytyrosol; wherein the pH of the aqueous solvent is adjusted by arginine. The modified hydroxytyrosol prepared by this method exhibits significantly enhanced free radical scavenging ability, can inhibit the expression of inflammatory factors TNF-α and IL-6, inhibit hyaluronidase activity, and thus possesses soothing and antioxidant effects. It is stable in an alkaline environment and also has moisturizing effects, making it suitable as an active ingredient in cosmetics, endowing cosmetics with at least one of the following effects: antioxidant, soothing, and moisturizing.