A fluffy oil control anti-dandruff composition, its preparation method and application

Abstract

The present application provides a fluffy oil-control anti-dandruff composition, a preparation method and application thereof, and relates to the technical field of cosmetics. The fluffy oil-control anti-dandruff composition comprises a plant composition and an anti-dandruff composition, wherein the plant composition is composed of Capra hircus, Terminalia chebula, flower / leaf of Tamarix and Artemisia annua in a mass ratio of (1-20):20:1:10, has the effects of synergistically inhibiting Malassezia and inhibiting hyaluronidase activity; the anti-dandruff composition comprises at least one of piroctone olamine, white willow bark and salvia miltiorrhiza extract, and is used for enhancing the effects of inhibiting bacteria, controlling oil and relieving. The fluffy oil-control anti-dandruff composition can simultaneously regulate the microecological balance of the scalp, and improve the problems of dandruff, excessive oil and scalp itching caused by sensitive scalp, and can be widely used in the preparation process of products for improving dandruff, excessive oil and scalp itching caused by sensitive scalp.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a fluffy, oil-controlling, and dandruff-removing composition, its preparation method, and its application. Background Technology

[0002] Sensitive scalp is a common and underestimated scalp problem, closely related to dandruff and hair loss. The incidence of sensitive scalp in China is approximately 35.77%. The scalp structure consists of the epidermis, dermis, and subcutaneous tissue from the outside in. Compared to other parts of the skin, the scalp has denser hair, abundant sweat and sebaceous glands, and a more significant interaction between sebum and microorganisms; at the same time, its stratum corneum is thinner, making its barrier function more susceptible to damage.

[0003] The pathogenesis of sensitive scalp is complex, resulting from the interaction of the microecology, skin barrier, and neurovascular system. At its core lies a vicious cycle of "microecological imbalance - skin barrier damage - neurovascular hyperreactivity." When the skin barrier function is impaired, sensory nerve signal transmission increases, triggering an immune inflammatory response. Many types of hair loss (such as androgenetic alopecia and alopecia areata) and dandruff are often accompanied by scalp sensitivity. An epidemiological study showed that approximately 32.18% of hair loss patients experienced symptoms of scalp sensitivity.

[0004] An imbalance in the scalp's microecology, particularly Malassezia, is a key factor contributing to dandruff, oily scalp, and hair loss. As the dominant fungi on the scalp, most Malassezia species are lipid-dependent. When the scalp secretes excessive sebum, Malassezia breaks down triglycerides and saturated fatty acids in the sebum into unsaturated fatty acids through the secretion of lipases. These unsaturated fatty acids can damage the barrier function of the stratum corneum, inducing the production of pro-inflammatory factors that cause scalp itching. They can also allow calcium ions to flow into scalp cells, further leading to excessive keratinization of scalp keratinocytes, thus exacerbating scalp problems.

[0005] A healthy scalp barrier acts as a "protective wall" to maintain scalp homeostasis. It is composed of sebum, keratinocytes, and natural moisturizing factors, effectively locking in moisture and resisting irritation. Once the barrier is damaged, two symptoms will appear: first, the ability to retain moisture will decrease, leading to dryness, tightness, and dandruff; second, it will cause excessive secretion of sebum by the sebaceous glands, resulting in oily scalp, flat scalp, and even seborrheic dermatitis.

[0006] Currently, mainstream chemical anti-dandruff and oil-controlling ingredients have inherent limitations. Commonly used chemical anti-dandruff and oil-controlling agents on the market include salicylic acid, selenium sulfide, zinc pyrithione (ZPT), piroctone olamine (OCT), guanylate, and clomiphene. Salicylic acid is a keratinocyte exfoliant, but it does not have significant antifungal effects, has poor dandruff removal, is highly irritating, and can easily peel off the skin, causing damage. Selenium sulfide is a cell growth inhibitor with strong irritation, causing dryness after use, and long-term use can lead to hair loss and odor. Zinc pyrithione (ZPT, which has been banned in the EU), piroctone olamine (OCT), clomiphene, and ketoconazole are all potent bactericides, but they all have drawbacks such as high irritation and environmental unfriendliness. Moreover, long-term use of chemical reagents can lead to drug resistance and harm the immune system. Long-term reliance on these chemical agents may have adverse effects, potentially leading to drug resistance in Malassezia and reduced dandruff-reducing efficacy. Furthermore, their irritant properties may further damage the scalp barrier, exacerbating nerve sensitization and microecological imbalance, creating a vicious cycle of "the more you use it, the oilier and itchier it becomes," and even worsening hair loss and dandruff problems. In conclusion, existing chemical methods fail to fundamentally solve scalp problems, especially the complexity of sensitive scalps.

[0007] Therefore, there is an urgent need in this field for an innovative solution that can address the root cause by synergistically working through multiple targets such as balancing the microecology, regulating sebum secretion, and inhibiting neurogenic pruritus, in order to achieve safe and long-term management of scalp problems.

[0008] In view of this, the present invention is hereby proposed. Summary of the Invention

[0009] The primary objective of this invention is to provide a fluffy, oil-controlling, and dandruff-removing composition. This composition, through the synergistic combination of a plant-based composition and a dandruff-removing composition, can simultaneously inhibit Malassezia, regulate the scalp's microecological balance, and inhibit hyaluronidase activity to alleviate neurovascular hyperreactivity. It also addresses oil control, dandruff removal, and soothing scalp itching, thereby comprehensively improving multiple problems caused by sensitive scalps.

[0010] A second objective of this invention is to provide a method for preparing the aforementioned fluffy, oil-controlling, and dandruff-removing composition.

[0011] A third objective of this invention is to provide an application of the aforementioned fluffy, oil-controlling, and dandruff-removing composition.

[0012] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted: This invention provides a fluffy, oil-controlling, and dandruff-removing composition, comprising a plant-based composition and an dandruff-removing composition, wherein: The plant composition consists of *Hemiberlesia lataniae*, *Terminalia chebula*, *Tamarix chinensis* flowers / leaves, and *Artemisia annua*, wherein the mass ratio of *Hemiberlesia lataniae*, *Terminalia chebula*, *Tamarix chinensis* flowers / leaves, and *Artemisia annua* is (1-20):20:1:10; The anti-dandruff composition contains at least one of piroctone olamine salt, white willow bark, and tanshinone extract.

[0013] Furthermore, the mass ratio of the sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf and Artemisia annua is 5:20:1:10.

[0014] Furthermore, the mass ratio of the plant composition to the anti-dandruff composition is 7:(5-11).

[0015] Furthermore, the anti-dandruff composition comprises piroctone ethanolamine salt, white willow bark, and tanshinone extract; Preferably, the mass ratio of the pyrrolidone ethanolamine salt, white willow bark, and tanshinone extract is 10:4:(1-7).

[0016] Furthermore, the tanshinone extract is a tanshinone extract loaded in nanomicelles.

[0017] The present invention provides a method for preparing the above-mentioned fluffy, oil-controlling, and dandruff-removing composition, the preparation method comprising: S1: Weigh out powders of sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua, and optionally white willow bark, add 8-15 times the amount of alcohol solution, soak for 0.5-2 hours, and then extract 1-4 times using any one of the following methods: ultrasound, reflux heating, or microfluidic extraction, each extraction lasting 10-60 minutes; after extraction, collect the filtrate to obtain the plant composition. S2: Add pyrrolidone ethanolamine salt and tanshinone nano micelles to the filtrate obtained in S1, stir to dissolve, mix well, and obtain the fluffy oil-controlling and dandruff-removing composition.

[0018] Furthermore, the alcohol solution is one of ethanol solution, 1,3-propanediol solution, propylene glycol solution, butanediol solution, 1,2-hexanediol solution or glycerol solution.

[0019] Preferably, the alcohol solution is a mixture of 70% butanediol and 30% water.

[0020] Furthermore, the preparation method includes: S1: Weigh out powders of sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua, and optional white willow bark. Add 8-15 times the amount of a mixed alcohol solution of 70% butanediol and 30% water, soak for 0.5-2 hours, and then extract 1-4 times with ultrasound at 50-70℃ and 30-50 kHz for 10-60 minutes each time. Filter and collect the filtrate. S2: Add pyrrolidone ethanolamine salt and tanshinone nano micelles to the filtrate obtained in S1, stir to dissolve, mix well, and obtain the fluffy oil-controlling and dandruff-removing composition.

[0021] The present invention provides the application of the above-mentioned fluffy oil-controlling and dandruff-removing composition in the preparation of products for improving dandruff, excessive scalp oil, and scalp itching caused by sensitive scalp.

[0022] Furthermore, the product includes any one of shampoo, conditioner, or scalp serum.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: The volumizing, oil-controlling, and dandruff-removing composition provided by this invention comprises a plant composition and an anti-dandruff composition. The plant composition is prepared from *Heliotropium indicum* root, *Terminalia chebula* fruit, *Tamarix chinensis* flower / leaf, and *Artemisia annua* in a mass ratio of (1-20):20:1:10. The anti-dandruff composition comprises at least one of piroctone olamine salt, *Salix babylonica* bark, and *Salvia miltiorrhiza* extract. Through the synergistic combination of the plant composition and the anti-dandruff composition, the volumizing, oil-controlling, and dandruff-removing composition can simultaneously inhibit *Malassezia*, regulate the scalp microecological balance, and inhibit hyaluronidase activity to alleviate neurovascular hyperreactivity. Furthermore, it also provides multiple benefits such as oil control, dandruff removal, and relief of scalp itching, thereby more comprehensively improving dandruff, excessive scalp oil, and scalp itching caused by sensitive scalps.

[0024] The present invention provides a method for preparing a fluffy, oil-controlling, and dandruff-removing composition. The method involves first soaking and gently extracting a plant composition from sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua powder, and optionally white willow bark powder in an alcohol solution. Then, at room temperature, piroctone olamine salt and tanshinone nanomicelles are added. This method avoids the degradation or inactivation of active ingredients in high-temperature or highly polar environments, and ensures the stable existence and effective synergy of each functional component. As a result, the final composition has multiple effects of antibacterial, oil-controlling, dandruff-removing, and soothing properties.

[0025] The above-mentioned fluffy oil-controlling and dandruff-removing composition provided by the present invention can be widely used in the preparation of products for improving dandruff, excessive scalp oil, and scalp itching caused by sensitive scalp. Detailed Implementation

[0026] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] According to one aspect of the present invention, a fluffy, oil-controlling, and dandruff-removing composition comprises a plant-based composition and an anti-dandruff composition, wherein: The plant composition consists of *Hemiberlesia lataniae*, *Terminalia chebula*, *Tamarix chinensis* flowers / leaves, and *Artemisia annua*, wherein the mass ratio of *Hemiberlesia lataniae*, *Terminalia chebula*, *Tamarix chinensis* flowers / leaves, and *Artemisia annua* is (1-20):20:1:10; The anti-dandruff composition contains at least one of piroctone olamine salt, white willow bark, and tanshinone extract.

[0028] The volumizing, oil-controlling, and dandruff-removing composition provided by this invention comprises a plant composition and an anti-dandruff composition. The plant composition is prepared from *Heliotropium indicum* root, *Terminalia chebula* fruit, *Tamarix chinensis* flower / leaf, and *Artemisia annua* in a mass ratio of (1-20):20:1:10. The anti-dandruff composition comprises at least one of piroctone olamine salt, *Salix babylonica* bark, and *Salvia miltiorrhiza* extract. Through the synergistic combination of the plant composition and the anti-dandruff composition, the volumizing, oil-controlling, and dandruff-removing composition can simultaneously inhibit *Malassezia*, regulate the scalp microecological balance, and inhibit hyaluronidase activity to alleviate neurovascular hyperreactivity. Furthermore, it also provides multiple benefits such as oil control, dandruff removal, and relief of scalp itching, thereby more comprehensively improving dandruff, excessive scalp oil, and scalp itching caused by sensitive scalps.

[0029] In a preferred embodiment of the present invention, the mass ratio of the sheep's milk root, Terminalia chebula, Tamarix flower / leaf and Artemisia annua is 5:20:1:10.

[0030] In a preferred embodiment, the present invention combines *Heliotropium indicum* root, *Terminalia chebula*, *Tamarix chinensis* flower / leaf, and *Artemisia annua* in a mass ratio of 5:20:1:10. This combination enhances the synergistic inhibitory effect of the plant composition on *Malassezia* and increases its inhibitory activity on hyaluronidase, thereby more effectively exerting the comprehensive effects of regulating scalp microecology, relieving neurovascular hyperreactivity, and improving symptoms related to sensitive scalp.

[0031] In a preferred embodiment of the present invention, the mass ratio of the plant composition to the dandruff-removing composition is 7:(5-11).

[0032] In a preferred embodiment, the present invention combines the plant composition with the anti-dandruff composition at a mass ratio of 7:(5-11), which helps to ensure the stability and compatibility of the system while ensuring synergistic antibacterial and soothing effects, thereby more effectively exerting the comprehensive care effects of fluffing, oil control, dandruff removal and relieving scalp itching.

[0033] In a preferred embodiment of the present invention, the anti-dandruff composition comprises piroctone ethanolamine salt, white willow bark, and tanshinone extract; Preferably, the mass ratio of the pyrrolidone ethanolamine salt, white willow bark, and tanshinone extract is 10:4:(1-7).

[0034] As a preferred embodiment, the anti-dandruff composition of the present invention is composed of piroctone olamine salt, white willow bark and tanshinone extract. The three components work synergistically to rapidly inhibit bacteria, regulate keratin metabolism and soothe neurovascular responses. Furthermore, when the three components are combined in a mass ratio of 10:4:(1-7), the ability to regulate the scalp microecology and soothe neurovascular hyperresponsiveness can be optimized while maintaining a good antibacterial effect.

[0035] In a preferred embodiment of the present invention, the tanshinone extract is a tanshinone extract loaded in nanomicelles.

[0036] In a preferred embodiment, the present invention loads tanshinone extract into nanomicelles, which can improve its dispersion stability and bioavailability in the composition system, thereby enhancing its synergistic effect with the plant composition and pyrrolidone ethanolamine salt, further optimizing its ability to regulate the scalp microecology and its soothing effect on neurovascular hyperresponsiveness.

[0037] According to one aspect of the present invention, a method for preparing the above-mentioned fluffy, oil-controlling, and dandruff-removing composition, the method comprising: S1: Weigh out powders of sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua, and optionally white willow bark, add 8-15 times the amount of alcohol solution, soak for 0.5-2 hours, and then extract 1-4 times using any one of the following methods: ultrasound, reflux heating, or microfluidic extraction, each extraction lasting 10-60 minutes; after extraction, collect the filtrate to obtain the plant composition. S2: Add pyrrolidone ethanolamine salt and tanshinone nano micelles to the filtrate obtained in S1, stir to dissolve, mix well, and obtain the fluffy oil-controlling and dandruff-removing composition.

[0038] The present invention provides a method for preparing a fluffy, oil-controlling, and dandruff-removing composition. The method involves first soaking and gently extracting a plant composition from sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua powder, and optionally white willow bark powder in an alcohol solution. Then, at room temperature, piroctone olamine salt and tanshinone nanomicelles are added. This method avoids the degradation or inactivation of active ingredients in high-temperature or highly polar environments, and ensures the stable existence and effective synergy of each functional component. As a result, the final composition has multiple effects of antibacterial, oil-controlling, dandruff-removing, and soothing properties.

[0039] In a preferred embodiment of the present invention, the alcohol solution is one of ethanol solution, 1,3-propanediol solution, propylene glycol solution, butanediol solution, 1,2-hexanediol solution or glycerol solution.

[0040] Preferably, the alcohol solution is a mixture of 70% butanediol and 30% water.

[0041] In a preferred embodiment of the present invention, the preparation method includes: S1: Weigh out powders of sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua, and optional white willow bark. Add 8-15 times the amount of a mixed alcohol solution of 70% butanediol and 30% water, soak for 0.5-2 hours, and then extract 1-4 times with ultrasound at 50-70℃ and 30-50 kHz for 10-60 minutes each time. Filter and collect the filtrate. S2: Add pyrrolidone ethanolamine salt and tanshinone nano micelles to the filtrate obtained in S1, stir to dissolve, mix well, and obtain the fluffy oil-controlling and dandruff-removing composition.

[0042] According to one aspect of the present invention, the above-mentioned fluffy oil-controlling and dandruff-removing composition is used in the preparation of products for improving dandruff, excessive scalp oil, and scalp itching caused by sensitive scalp.

[0043] The above-mentioned fluffy oil-controlling and dandruff-removing composition provided by the present invention can be widely used in the preparation of products that improve dandruff, excessive scalp oil, and scalp itching caused by sensitive scalp.

[0044] In a preferred embodiment of the present invention, the product includes any one of shampoo, conditioner, or scalp serum.

[0045] The technical solution of the present invention will be further described below with reference to the embodiments.

[0046] Note: In the following embodiments and comparative examples of this application, the tanshinone extract used is tanshinone extract loaded in nano micelles, with the trade name Ganyin Qingdou, supplied by Sida (Guangdong) Biomedical Technology Co., Ltd., and the product batch number in the patent test is GDSDY250731.

[0047] Note: In the following embodiments and comparative examples of this application, the particle size of sheep milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua, white willow bark, and ordinary Salvia miltiorrhiza powder is 0.1~4 mm.

[0048] Example 1: Single Plant Extract This embodiment discloses a fluffy, oil-controlling, and dandruff-removing plant composition, which consists of sheep's milk root. The sheep's milk root was provided by Star (Guangdong) Biomedical Technology Co., Ltd.

[0049] Specifically, the preparation method of the sheep milk root includes the following steps: Weigh 17.64 g of *Sheep's Milk Root* powder and add 10 times the amount (based on the weight of the medicinal material) of an alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water). Soak for 1 hour, then extract by ultrasonication at 60℃ and 40 kHz for 30 minutes. Filter and collect the filtrate. Add alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water) to a total mass of 100 g and mix thoroughly to obtain a *Sheep's Milk Root* extract with a mass fraction of 17.64%, referred to as Example 1.

[0050] Examples 2-5 and Comparative Examples 1-8: compound plant compositions A fluffy, oil-controlling, and dandruff-removing plant composition is formulated from sheep's milk root, Terminalia chebula, Tamarix chinensis flowers / leaves, and Artemisia annua. The specific components and contents of each embodiment and comparative example are shown in Table 1.

[0051] Table 1:

[0052] The preparation method of the compound plant composition includes the following steps: Weigh out 17.64 g of the compound plant composition powder (the proportions of each plant are shown in Table 1), add 10 times the amount (based on the weight of the medicinal materials) of alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water), soak for 1 hour, and then extract by ultrasonication at 60℃ and 40 kHz for 30 minutes. Filter and collect the filtrate, then add alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water) to make up to 100 g of total mass, mix thoroughly, and you will get the compound plant composition with a mass fraction of 17.64%.

[0053] Comparative Examples 9-14 To verify the specificity of the selected compositions, control compositions were prepared by replacing them with other common plant extracts. The specific components and contents of each control composition are shown in Table 2.

[0054] Table 2:

[0055] Note: " / " indicates that the ingredient is not added.

[0056] The preparation method of the compound plant composition in the comparative example is the same as that in Example 2.

[0057] Examples 6-9 This embodiment discloses a fluffy, oil-controlling, and dandruff-removing composition, which is compounded from a plant composition and a dandruff-removing composition containing tanshinone extract nanomicelles. The ratio of plant composition to dandruff-removing composition is 7:(5~11), and the total content of the composition is 40.32%, wherein: Plant composition: The composition adopts the proportion of the components in Example 3, that is, it is composed of milkweed root, Terminalia chebula, Tamarix flower / leaf, and Artemisia annua in a ratio of 5:20:1:10.

[0058] Anti-dandruff composition: composed of OCT (pyrrolidone ethanolamine salt), white willow bark, and tanshinone extract in a ratio of 10:4:1; wherein, the tanshinone extract is a tanshinone extract loaded in nano micelles, with the trade name Gan Yin Qing Dou, supplied by Sida (Guangdong) Biomedical Technology Co., Ltd., and the product batch number under patent testing is GDSDY250731.

[0059] The formulations of the plant-based composition and the anti-dandruff composition are shown in Table 3.

[0060] Table 3:

[0061] The preparation method of the fluffy, oil-controlling, and dandruff-removing composition includes the following steps: S1. Weigh each sample according to the proportions shown in Table 3 to prepare a compound system with a total content of 40.32%. This system is composed of a plant composition and an anti-dandruff composition containing tanshinone extract nanomicelles.

[0062] S2. Weigh out powders of sheep's milk root, Terminalia chebula, Tamarix flower / leaf, Artemisia annua, and white willow bark, and add 10 times the amount (based on the total mass of the medicinal materials) of an alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water), soak for 1 hour, and then extract once by ultrasound at 60℃ and 40 kHz for 30 minutes each time; filter and collect the filtrate. S3. Weigh out OCT (pyrrolidone ethanolamine salt) and tanshinone nanomicelles and add them to the filtrate collected in S2. Stir to dissolve. Add alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water) to make up to a total mass of 100 g. Mix thoroughly to obtain a fluffy oil-controlling and dandruff-removing composition with a mass fraction of 40.32%.

[0063] Example 10 Except that the ratio of OCT (pyrrolidone ethanolamine salt), white willow bark, and salvia miltiorrhiza extract in the dandruff composition is 10:4:2, this embodiment is the same as in Example 7.

[0064] Example 11 Except that the ratio of OCT (pyrrolidone ethanolamine salt), white willow bark, and salvia miltiorrhiza extract in the dandruff composition is 10:4:3, this embodiment is the same as in Example 7.

[0065] Example 12 Except that the ratio of OCT (pyrrolidone ethanolamine salt), white willow bark, and salvia miltiorrhiza extract in the dandruff composition is 10:4:5, this embodiment is the same as in Example 7.

[0066] Example 13 Except that the ratio of OCT (pyrrolidone ethanolamine salt), white willow bark, and salvia miltiorrhiza extract in the dandruff composition is 10:4:7, this embodiment is the same as in Example 7.

[0067] Comparative Examples 15-19 To verify the synergistic effect of the tanshinone nanomicelle carrier and the plant composition, Comparative Examples 15-19 were made by replacing the "tanshinone nanomicelles" with ordinary tanshinone powder with the same content of active ingredients. The other components, proportions and preparation methods were the same as in Example 7, and the total amount of butanediol and water was adjusted accordingly to make up 100%.

[0068] Table 4:

[0069] The preparation method of the fluffy, oil-controlling, and dandruff-removing composition includes the following steps: S1. Weigh each sample according to the proportions shown in Table 4 (to prepare a compound system with a total content of 40.32%).

[0070] S2. Weigh out the following herbs: sheep's milk root, Terminalia chebula, Tamarix flower / leaf, Artemisia annua, white willow bark, and Salvia miltiorrhiza powder. Add 10 times the amount (based on the total mass of the herbs) of an alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water), soak for 1 hour, and then extract once by ultrasonication at 60℃ and 40 kHz for 30 minutes each time. Filter and collect the filtrate. S3. Weigh out OCT (pyrrolidone ethanolamine salt) and add it to the filtrate collected in S2. Stir to dissolve. Make up the total mass to 100 g with an alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water). Mix thoroughly to obtain comparative examples 15-19 with a mass fraction of 40.32%.

[0071] Comparative Example 20 This comparative example uses a simple system containing only OCT to compare the contributions of plant compositions and tanshinone nanomicelles.

[0072] The preparation method of Comparative Example 20 includes the following steps: Weigh 15.12 g of OCT (pyrrolidone ethanolamine salt) (the same OCT content as in Example 7 and Comparative Example 15), add an alcohol solution (a mixed alcohol solution of 70% butanediol and 30% water) to a total mass of 100 g, and mix thoroughly to obtain the final product.

[0073] Test Example 1 This test evaluated the inhibitory effect (regulatory effect on the microecology) of each test drug on Malassezia by determining the minimum inhibitory concentration (MIC) of each example and comparative example using the microbroth dilution method; and evaluated the synergistic antibacterial effect of the composition by exploring the synergistic effect index (CI).

[0074] 1. Test strains: Malassezia furfur (ATCC44344) was purchased from the Shanghai Microbial Culture Collection Center and is a publicly available biological material. This invention uses it only as an experimental test strain and does not involve the preservation of this strain as a biological material. Therefore, no preservation certificate or survival certificate is required.

[0075] 2. Testing Method: (1) Preparation of modified Dxion (mDixon) medium and modified Dxion (mDixon) agar medium: According to the instructions, accurately weigh the medium granules, agar powder and deionized water into a beaker, stir with a glass rod until the granules are completely dissolved, adjust the pH value to 6.1±0.1, dispense into conical flasks, seal, autoclave at 121℃ for 30 min, cool and store in a refrigerator at 4℃ for later use.

[0076] (2) Preparation of bacterial suspension: Take the glycerol tubes of the test bacteria stored in a -20℃ freezer, use an inoculation loop to take 20 μL of bacterial suspension, and inoculate it onto an agar slant using the streak method. Incubate in a 32℃ incubator for 2 days. Rinse the slant with physiological saline to obtain a high-concentration bacterial suspension. Take another test tube, pour in an appropriate amount of raw salt, and take an appropriate amount of the high-concentration bacterial suspension until its turbidity is consistent with that of a No. 5 McFarland turbidity tube, thus obtaining a final concentration of 1×10⁻⁶. 6 CFU / mL bacterial suspension.

[0077] (3) Determination of MIC value: Culture method: Add 100 μL of modified Dixon (mDixon) liquid medium to each of columns 2-11 of a 96-well plate, and add 200 μL of modified Dixon (mDixon) liquid medium to column 12. Add 200 μL of each test drug to column 1, and take 100 μL for horizontal dilution to column 10 (discard the excess 100 μL of drug solution).

[0078] Then, the bacterial suspension prepared in (2) was added to columns 1-11, 100 μL per well. At this point, the final volume of each well was 200 μL. Column 11 was the growth control well, and column 12 was the blank control well. The 96-well plate was incubated at 32℃ for 1-2 days.

[0079] Evaluation criteria: Visual observation shows that the concentration of the drug solution in the sterile growth well is the minimum inhibitory concentration (MIC).

[0080] (4) Synergistic antibacterial test and evaluation: Chou The Talalay model is a commonly used pharmacological model for evaluating the synergistic and antagonistic effects of compound combinations during the onset of action. The MICs of each embodiment were measured according to the method described in (3), and then analyzed by Chou. The Talalay formula calculates the synergy index (CI) to determine whether synergy exists. The formula is as follows:

[0081] Evaluation criteria: C a C b C c C d These represent the concentrations corresponding to the antibacterial effects of components A, B, C, and D acting individually; C A C B C C C D C represents the inhibitory effect observed at corresponding concentrations when the individual components are combined into a composition. A D Result = MIC experimental data of composition × C A D The proportion of a component in the composition. CI < 1 indicates a synergistic effect; CI = 1 indicates no interaction; CI > 1 indicates an antagonistic effect.

[0082] 3. Test Results: 3.1 Table 5 shows the MIC of sheep milk root extract (Example 1) against Malassezia.

[0083] Table 5. Inhibition results of sheep milk root extract (Example 1) against Malassezia:

[0084] As shown in Table 5, the minimum inhibitory concentration of sheep milk root extract against Malassezia is 12.5%, indicating a good inhibitory effect on Malassezia, a pathogenic bacterium of dandruff.

[0085] 3.2 Table 6 shows the inhibition results of Examples 2-5 and Comparative Examples 1-14 on Malassezia.

[0086] Table 6. Inhibition results of plant compositions on Malassezia:

[0087] As can be seen from Examples 2-5 and Comparative Examples 1-8, when the ratio of sheep milk root: Terminalia chebula: Tamarix flower / leaf: Artemisia annua is (1-20): 20: 1: 10, the synergistic index CI < 1, indicating that the composition has a synergistic effect, and the antibacterial ability of the composition is significantly better than that of the single sheep milk root extract.

[0088] Any imbalance in the proportions of any component (as shown in Comparative Examples 1-8) will lead to the loss of synergistic effects, or even antagonistic effects (CI>1) or a decrease in efficacy (increased MIC). Specifically: (1) Increasing the proportion of *Heliotropium indicum* within a certain range can enhance synergy (decreased CI), but excessive amounts (such as in Comparative Example 1) can lead to antagonism. Increasing the ratio of *Tamarix chinensis* flowers / leaves can significantly disrupt synergy, leading to antagonism and increased MIC (Comparative Examples 2-5). Too low a proportion of *Terminalia chebula* can lead to antagonism and increased MIC (Comparative Examples 6-7). Increasing the proportion of *Artemisia annua* can lead to antagonism (Comparative Example 8).

[0089] (2) The substitution experiments of Comparative Examples 9-13 demonstrate that the synergistic combination of this application has high specificity and cannot be replaced by other common plant extracts (Magnolia lily, Schisandra chinensis, Scutellaria baicalensis, Sapindus mukorossi). Replacing Tamarix chinensis flowers / leaves (Comparative Example 9) or Terminalia chebula (Comparative Examples 10-12) resulted in the disappearance of the synergistic effect, turning it into antagonism or no effect. Even when retaining the four core components, adding other extracts (Comparative Examples 13-14) would destroy the synergy and produce antagonism.

[0090] In summary, the four extracts of *Heliotropium indicum*, *Terminalia chebula*, *Tamarix chinensis* flower / leaf, and *Artemisia annua* described in the test examples exhibited a clear synergistic antibacterial effect (CI < 1) within a specific ratio range (1–20:20:1:10), and the effect was significantly better than that of a single *Heliotropium indicum* extract. This synergistic effect is highly sensitive to the ratio of each component; deviation from the ratio of any component or substitution of any component with other common plants will lead to a weakening or even disappearance of the synergistic effect, resulting in antagonistic effects (CI > 1) or an increase in MIC.

[0091] Test Example 2 This test evaluates the inhibitory ability and sensitivity of each test drug against Malassezia by using inhibition zones.

[0092] 1. The preparation of the test strains and bacterial suspensions is the same as in Test Example 1.

[0093] 2. Antibacterial test: Dip a sterile cotton swab in the bacterial suspension and spread it evenly three times on the surface of the culture medium plate. Place a blank antibiotic susceptibility test strip onto the prepared culture medium plate using sterile forceps. Use a micropipette to transfer 20 μL of the test sample onto the blank antibiotic susceptibility test strip. Cover the plate and incubate upright in a 32°C biochemical incubator for 48 hours. Observe the results. Measure and record the diameter of the inhibition zone using calipers.

[0094] 3. Evaluation criteria: According to the "Standards for the Implementation of Antimicrobial Susceptibility Testing" issued by the National Committee for Clinical Laboratory Standards (NCCLS) in the United States, and combined with the criteria for judging the results of the inhibition zone test in WS / T650—2019 "Methods for Evaluating Antimicrobial and Bacteriostatic Efficacy": an inhibition zone diameter >20 mm indicates extremely sensitive, 15–20 mm indicates highly sensitive, 10–15 mm indicates moderately sensitive, 7–10 mm indicates lowly sensitive, and ≤7 mm indicates no antimicrobial activity.

[0095] 4. Test Results: Table 7 shows the antibacterial zone results of the synergistic compound system based on nanocarriers (Examples 6-9). This system is composed of a plant composition and an anti-dandruff composition containing tanshinone extract nanomicelles, with a plant composition:anti-dandruff composition ratio of 7:(5-11), and a total composition content of 40.32%. The plant composition is in the ratio described in Example 3 (i.e., *Heliotropium indicum* root, *Terminalia chebula*, *Tamarix chinensis* flower / leaf, and *Artemisia annua* in a ratio of 5:20:1:10), and the anti-dandruff composition ratio is OCT:*White willow bark:*Tanshinone extract = 10:4:1.

[0096] Table 7. Antibacterial zone results of the synergistic compound system 1 based on nanocarriers (Examples 6-9):

[0097] Table 8 shows the antibacterial zone results of the synergistic compound system 2 based on nanocarriers (Examples 7, 10-13). This system is composed of a plant composition and an anti-dandruff composition containing tanshinone extract nanomicelles, with a plant composition:anti-dandruff composition ratio of 7:9 and a total composition content of 40.32%. The plant composition is in the ratio described in Example 3 (i.e., *Heliotropium indicum* root, *Terminalia chebula*, *Tamarix chinensis* flower / leaf, and *Artemisia annua* in a ratio of 5:20:1:10), and the total content of the plant composition is 17.64%. The anti-dandruff composition ratio is OCT:*White willow bark:*tanshinone extract = 10:4:(1-7), and the total content of the anti-dandruff composition is 22.68%.

[0098] Table 8. Antibacterial zone results of the synergistic compound system based on nanocarrier 1 (Examples 7, Examples 10-13):

[0099] Table 9 shows the inhibition zone results for Comparative Examples 15-19. Comparative Examples 15-19 are identical to Example 7 except that the "Salvia miltiorrhiza nanomicelles" were replaced with ordinary Salvia miltiorrhiza extract with the same content of active ingredients. The total amounts of butylene glycol and water were adjusted accordingly to make up 100%.

[0100] Table 9 shows Comparative Example 20, a simple system containing only OCT, used to compare the contributions of plant compositions and tanshinone nanomicelles.

[0101] Table 9. Results of inhibition zones in the comparative examples:

[0102] Table 9 shows that, with the same OCT addition amount (15.120%), the inhibition zones of Comparative Example 20, Comparative Example 15, and Example 7 were 15.83 mm, 16.17 mm, and 17.83 mm, respectively. This indicates that adding plant compositions to OCT, as well as upgrading ordinary Salvia miltiorrhiza extract to Salvia miltiorrhiza nanomicelles, can effectively enhance the antibacterial effect, and the synergistic effect of the compound system of plant compositions and Salvia miltiorrhiza nanomicelles is the most significant.

[0103] Furthermore, comparing the data of Examples 7, 10-13, and Comparative Examples 15-19 under the same OCT, white willow bark, and Salvia miltiorrhiza ratio, the examples using Salvia miltiorrhiza nanomicelles all showed superior inhibition zones compared to their corresponding comparative examples. For example, Example 7 (17.83 mm) > Comparative Example 15 (16.17 mm); Example 11 (16.33 mm) > Comparative Example 17 (15.17 mm). This indicates that compared to ordinary extract forms, Salvia miltiorrhiza nanomicelle carriers can more effectively enhance the antibacterial activity of the entire compound system. This is likely due to the fact that nanomicelle technology improves the solubility and bioavailability of the active ingredients in Salvia miltiorrhiza.

[0104] Furthermore, in the tests shown in Table 7, when the plant composition formulation (Example 3 ratio) and the internal ratio of the desiccant composition (OCT: white willow bark: salvia miltiorrhiza = 10:4:1) were fixed, and the mixing ratio of the plant composition and the desiccant composition was changed, the diameter of the inhibition zone of all samples (Examples 6-9) was greater than 15 mm, all reaching a highly sensitive level. This proves that within this ratio range, the system can maintain excellent antibacterial performance.

[0105] Of particular note is that, even with reduced OCT dosage, the compound system still exhibits antibacterial effects superior to or equivalent to those of a single OCT system. For example, in Example 8, the OCT content was 1.68% lower than in Comparative Example 20, but its antibacterial sensitivity (16.33 mm) was still superior to the single OCT system (15.83 mm); in Example 9, the OCT content was reduced by 3.92%, but its inhibition zone (15.33 mm) was still comparable to that of the single OCT system. This demonstrates that the compound composition of the present invention can effectively enhance the antibacterial efficacy of the chemical antidandruff agent OCT, providing a possibility for reducing OCT dosage and developing gentler products while maintaining or even improving the antidandruff effect.

[0106] In summary, this invention utilizes a specific quaternary plant composition, scientifically formulated with a dandruff-reducing composition based on tanshinone nanomicelles, to construct a highly efficient, multi-level synergistic system. This system not only leverages the clear synergistic antibacterial effect of the plant composition itself but also significantly enhances the antibacterial activity of the chemical dandruff agent OCT, and further improves the efficacy of the active ingredients through nanocarrier technology. This invention provides an innovative solution for developing highly efficient, gentle, and multi-target scalp care products.

[0107] Test Example 3 This test analyzes the effects of each test sample on neurovascular hyperreactivity and inflammatory response by measuring the hyaluronidase inhibition rate.

[0108] There is a close interaction between hyaluronidase and neurovascular system disorders, neurovascular hyperresponsiveness, and skin inflammation: Hyaluronidase increases vascular permeability and capillary fragility by degrading hyaluronic acid in the extracellular matrix, promoting the migration of inflammatory cells to tissues, thereby exacerbating the skin inflammatory response; at the same time, under neurovascular hyperresponsiveness, neuropeptides (such as substance P, CGRP, etc.) released by sensory nerve endings can induce mast cell degranulation and release inflammatory mediators such as histamine and trypsin-like proteins. These mediators further upregulate hyaluronidase activity, forming a vicious cycle amplification mechanism of "neuropeptide-mast cell-hyaluronidase"; and excessively high hyaluronidase activity can damage the tissue barrier, making it easier for vascular regulatory factors and inflammatory mediators to spread, affecting the sympathetic / parasympathetic nerve regulation of blood vessels, leading to abnormal vasomotor responses, and ultimately manifesting as neurovascular disorder symptoms such as paroxysmal flushing, persistent erythema, and telangiectasia.

[0109] Therefore, hyaluronidase plays a crucial amplifying role in this pathological network, and inhibiting its activity has become one of the important strategies for treating neurovascular inflammatory diseases. Furthermore, hyaluronidase is also associated with most IgE-mediated type I and T cell-mediated type IV hypersensitivity reactions.

[0110] 1. Testing method: Enzyme and CaCl2 were added sequentially to the reaction tube and incubated at 37°C for 20 minutes. The sample to be tested was then added, and the reaction was continued at 37°C for another 20 minutes. Hyaluronic acid solution was then added, and the reaction was continued at 37°C for 30 minutes. After the reaction was complete, acetylacetone and sodium hydroxide solution were added sequentially, and the mixture was cooled in a boiling water bath followed by an ice bath. Finally, the colorimetric reagent was added, and the absorbance was measured at 530 nm.

[0111] 2. Calculation method: Hyaluronidase inhibition rate (%) = [1 - (AB) / (CD)] × 100% In the formula: A is the absorbance of the experimental group (calcium chloride solution, hyaluronidase solution, sample solution (sample group) / water (negative control group / dipotassium glycyrrhizate solution (positive control group)); B represents the absorbance of the experimental blank group (calcium chloride solution, acetate buffer, sample solution (sample group) / water (negative control group / dipotassium glycyrrhizate solution (positive control group)); C represents the absorbance of the control group (calcium chloride solution, acetate buffer, hyaluronidase solution); D represents the absorbance of the blank group (calcium chloride solution, acetate buffer).

[0112] 3. Test Results: (1) Table 10 shows the inhibitory effect of sheep milk root extract (Example 1) on hyaluronidase.

[0113] Table 10:

[0114] As shown in Table 10, the inhibition rate of the sheep milk root extract in Example 1 of the present invention against hyaluronidase reached 33.33%, which was statistically different from the negative control group (p < 0.05). This indicates that the sheep milk root extract has the ability to inhibit hyaluronidase activity at the tested concentration, which can be used as one of the evidences supporting the claim that the sample has a soothing effect. Furthermore, the sheep milk root extract can alleviate neurovascular inflammatory diseases and pruritus by inhibiting hyaluronidase.

[0115] (2) Table 11 shows the inhibitory effect of other test samples on hyaluronidase.

[0116] Table 11:

[0117] As shown in Table 11, the inhibition rate of hyaluronidase in Example 3 was significantly better than that of sheep milk root extract alone, indicating that the composition has a synergistic effect and can significantly inhibit hyaluronidase activity, thereby alleviating neurovascular inflammatory diseases and pruritus.

[0118] Table 11 further compares the hyaluronidase inhibition rates of Comparative Examples 20, 15, and 7 under the same OCT addition conditions. The results show that the hyaluronidase inhibition rate of Comparative Example 15 was slightly higher than that of Comparative Example 20; while the inhibition rate of Example 7 was higher than that of Comparative Example 15. This indicates that introducing a plant composition based on OCT and replacing ordinary tanshinone extract with tanshinone nanomicelles can significantly enhance the composition's inhibitory ability on hyaluronidase, i.e., enhance the inhibitory effect on neurovascular hyperreactivity and pruritus response.

[0119] Application Example 1 This application uses the compositions of Example 7, Comparative Example 15, and Comparative Example 20 as examples to test the application effect of the compositions in shampoo products.

[0120] (1) Prepare a shampoo for improving problems such as oily scalp, dandruff, and itchy scalp. The weight parts of each component are shown in Table 12. The preparation method includes the following steps: S1. Add A1 phase water to the main pot, add the remaining A1 phase raw materials under homogenization, disperse completely, add A2 phase raw materials, stir thoroughly, heat to 80~85°C, add A3 phase raw materials and stir until completely dissolved; S2. Add phase B1 and stir thoroughly until completely dissolved. Then add phase B2 and stir until completely dissolved. S3. Cool down to 45~50℃, add the C phase raw materials in sequence, and stir evenly; S4. Add the D phase raw materials in sequence, adjust the pH value and consistency, and stir until well mixed. S5. Sampling and testing; material is discharged if the test is qualified.

[0121] Table 12 Shampoo Formulas:

[0122] (2) Consumer testing: Ninety male and female volunteers aged 18 to 50 were recruited (selection criteria were healthy volunteers with oily scalp, dandruff, and itchy scalp). Each group consisted of 30 people. The volunteers used the shampoo every other day (once every two days) for four consecutive weeks. They were evaluated on three aspects: hair volume, dandruff control, and itch relief. The rating range was 0-7 (7 for extremely satisfied, 6 for very satisfied, 5 for fairly satisfied, 4 for neutral, 3 for somewhat dissatisfied, 2 for very dissatisfied, and 1 for extremely dissatisfied). Higher scores indicated greater agreement. Overall satisfaction refers to the percentage of participants who rated their scalp between 5 and 7. The consumer test results are shown in Table 13.

[0123] Table 13 Consumer Test Results:

[0124] As shown in Table 13, under the premise of the same OCT addition amount, Example 7 is superior to Comparative Example 15 and Comparative Example 20 in terms of hair volume, dandruff removal, and itch relief. This further verifies that adding specific quaternary plant compositions to OCT, and upgrading the ordinary tanshinone extract in the dandruff removal composition to tanshinone nanomicelles, can effectively enhance the antibacterial, oil-controlling, and itch-relieving effects, and the compound system of plant composition and tanshinone nanomicelles has the most significant synergistic effect.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fluffy, oil-controlling, and dandruff-removing composition, characterized in that, It comprises a plant-based composition and an anti-dandruff composition, wherein: The plant composition consists of *Hemiberlesia lataniae*, *Terminalia chebula*, *Tamarix chinensis* flowers / leaves, and *Artemisia annua*, wherein the mass ratio of *Hemiberlesia lataniae*, *Terminalia chebula*, *Tamarix chinensis* flowers / leaves, and *Artemisia annua* is (1-20):20:1:10; The anti-dandruff composition contains at least one of piroctone olamine salt, white willow bark, and tanshinone extract.

2. The fluffy, oil-controlling, and dandruff-removing composition according to claim 1, characterized in that, The mass ratio of the following ingredients is 5:20:1:10: *Heliotropium indicum* root, *Terminalia chebula* fruit, *Tamarix chinensis* flower / leaf, and *Artemisia annua*.

3. The fluffy, oil-controlling, and dandruff-removing composition according to claim 1, characterized in that, The mass ratio of the plant composition to the anti-dandruff composition is 7:(5-11).

4. The fluffy, oil-controlling, and dandruff-removing composition according to claim 1, characterized in that, The anti-dandruff composition consists of piroctone ethanolamine salt, white willow bark, and salvia miltiorrhiza extract; Preferably, the mass ratio of the pyrrolidone ethanolamine salt, white willow bark, and tanshinone extract is 10:4:(1-7).

5. The fluffy, oil-controlling, and dandruff-removing composition according to claim 4, characterized in that, The tanshinone extract is a tanshinone extract loaded in nanomicelles.

6. A method for preparing the fluffy, oil-controlling, and dandruff-removing composition according to any one of claims 1 to 5, characterized in that, The preparation method includes: S1: Weigh out powders of sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua, and optionally white willow bark, add 8-15 times the amount of alcohol solution, soak for 0.5-2 hours, and then extract 1-4 times using any one of the following methods: ultrasound, reflux heating, or microfluidic extraction, each extraction lasting 10-60 minutes; after extraction, collect the filtrate to obtain the plant composition. S2: Add pyrrolidone ethanolamine salt and tanshinone nano micelles to the filtrate obtained in S1, stir to dissolve, mix well, and obtain the fluffy oil-controlling and dandruff-removing composition.

7. The method for preparing the fluffy, oil-controlling, and dandruff-removing composition according to claim 6, characterized in that, The alcohol solution is one of ethanol solution, 1,3-propanediol solution, propylene glycol solution, butanediol solution, 1,2-hexanediol solution or glycerol solution; Preferably, the alcohol solution is a mixed alcohol solution of 70% butanediol and 30% water.

8. The method for preparing the fluffy, oil-controlling, and dandruff-removing composition according to claim 6, characterized in that, The preparation method includes: S1: Weigh out powders of sheep's milk root, Terminalia chebula, Tamarix chinensis flower / leaf, Artemisia annua, and optional white willow bark. Add 8-15 times the amount of a mixed alcohol solution of 70% butanediol and 30% water, soak for 0.5-2 hours, and then extract 1-4 times with ultrasound at 50-70℃ and 30-50 kHz for 10-60 minutes each time. Filter and collect the filtrate. S2: Add pyrrolidone ethanolamine salt and tanshinone nano micelles to the filtrate obtained in S1, stir to dissolve, mix well, and obtain the fluffy oil-controlling and dandruff-removing composition.

9. The use of the volumizing, oil-controlling, and dandruff-removing composition according to any one of claims 1 to 5 in the preparation of products for improving dandruff, excessive scalp oil, and scalp itching caused by sensitive scalp.

10. The application according to claim 9, characterized in that, The product includes any one of shampoo, conditioner, or scalp serum.

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