Soothing, firming and anti-wrinkle composition, application and gel for fading cheilogramma

Abstract

The invention belongs to the technical field of skin care, and discloses a soothing, firming and anti-wrinkle composition, application and gel for fading cheilogramma The soothing, firming and anti-wrinkle composition is prepared from, by weight, 5-15 parts of hydrogenated polyisobutene, 0.1-1 part of citron fruit extract and 0.1-3 parts of peony seed oil. The hydrogenated polyisobutene, the citron fruit extract and the peony seed oil are compounded, so that the hyaluronidase activity inhibition rate and the DPPH free radical scavenging rate of the composition are improved, and the effects of soothing the skin, tightening the skin and resisting wrinkles can be achieved.

Description

Technical Field

[0001] The present application relates to the technical field of skin care, and in particular to a soothing, firming and anti-wrinkle composition, its use, and a gel for lightening lip lines. Background Art

[0002] With the intensification of global aging and the upgrading of consumers' demand for health and beauty, skin anti-aging technology has moved from traditional skin care to multimodal collaborative treatment; skin soothing, firming and anti-wrinkle are the three core demands for maintaining skin health and youthfulness; now there are different skin care products on the market that provide skin care for soothing, firming and anti-wrinkle respectively.

[0003] For example, prior art 1: Chinese patent application 202410617453.2 discloses a whitening, anti-wrinkle, firming, and soothing multi-effect composition, the raw materials of which include the following components: niacinamide, hydroxypropyl tetrahydropyrantriol, p-hydroxyacetophenone, glycerin, 1,2-pentanediol, 1,2-hexanediol, a barley extract composition, panthenol, a gentian root extract composition, an oat kernel extract composition, and the remainder water. The addition of barley extract, gentian root extract, and oat kernel extract to the whitening, anti-wrinkle, firming, and soothing multi-effect composition of the present invention can inhibit the transfer of melanosomes to the skin surface, repair UV-induced DNA damage, intervene in photoimmunosuppression, inhibit elastase, and inhibit hyaluronidase. The facial cream prepared using the whitening, anti-wrinkle, firming, and soothing multi-effect composition of the present invention has whitening, anti-wrinkle, firming, and soothing effects.

[0004] Similarly, in prior art 2, Chinese patent application 202311538944.X discloses a moisturizing, anti-wrinkle, soothing, and firming composition and a cream containing the same. The moisturizing, anti-wrinkle, soothing, and firming composition comprises the following components: crocus extract, carnation flower extract, Spirulina maxima extract, a humectant, and a solvent. When incorporated into a cream, the composition provides superior moisturizing, anti-wrinkle, soothing, and firming benefits, potentially addressing the problem of current skincare products lacking the functionality to meet facial needs.

[0005] Prior Art 2 mentioned that the skin care products on the current market have a single function and cannot meet people's facial needs. Based on technological development, Prior Art 1 and Prior Art 2 already have multifunctional skin care, but they do not meet consumers' diverse needs for skin care products. Therefore, it is necessary to develop more different skin care compositions that can achieve multifunctional skin care while meeting the diverse needs of the market. Summary of the Invention

[0006] One of the purposes of the present application is to provide a soothing, firming and anti-wrinkle composition. By compounding the components, the soothing effect of the composition can be effectively improved, and it has a higher firming and anti-wrinkle effect, providing skin care products with diverse effects while providing more diverse choices for the market.

[0007] Another object of the present application is to provide a use of a soothing, firming and anti-wrinkle composition, which is used to prepare skin care products, so that the skin care products can have soothing, firming and anti-wrinkle effects.

[0008] Another purpose of the present application is to provide a gel for lightening lip lines, which contains the soothing, firming and anti-wrinkle composition of the present application and has excellent lip line lightening effect.

[0009] To achieve the above objectives, the present application provides a soothing, firming and anti-wrinkle composition, which comprises 5-15 parts of hydrogenated polyisobutene, 0.1-1 parts of citron fruit extract and 0.1-3 parts of peony seed oil, by weight.

[0010] The present application also provides the use of the soothing, firming and anti-wrinkle composition in preparing skin care products.

[0011] Preferably, the skin care product comprises at least one of gel, cream, oil, essence and lotion.

[0012] The present application also provides a gel for lightening lip lines, comprising 0.1-99 wt % of the above-mentioned soothing, firming and anti-wrinkle composition.

[0013] Beneficial effects

[0014] Compared with the existing technology, the present application provides a soothing, firming and anti-wrinkle composition. By compounding hydrogenated polyisobutene, citron fruit extract and peony seed oil, the hyaluronidase activity inhibition rate and DPPH free radical scavenging rate of the composition are improved, which can bring skin soothing, skin firming and anti-wrinkle effects. DETAILED DESCRIPTION

[0015] The present application is further described below in conjunction with embodiments, but does not constitute any limitation to the present application. Any limited modifications made within the scope of the claims of the present application are still within the scope of the claims of the present application.

[0016] In order to explain the technical content of this application in detail, further explanation is given below in conjunction with the implementation methods.

[0017] In the following examples and comparative examples, the hydrogenated polyisobutene was purchased from Muyang County Changjiang Oil Co., Ltd.; the citron fruit extract was purchased from Guangzhou Xinsimei Scientific Research Co., Ltd.; the peony seed oil was purchased from Heze Guyu Peony Biotechnology Co., Ltd.; and the jojoba seed oil was purchased from JOJOBA DESERT (ACS) LTD.

[0018] It should be noted that the raw materials used in the technical solution of this application can be obtained through other commercially available products and are not limited to the above sources.

[0019] Unless otherwise specified, in the following examples and comparative examples, parts and % refer to parts by weight and percentage by weight, respectively.

[0020] Example 1 A soothing, firming and anti-wrinkle composition is obtained by mixing 15 parts of hydrogenated polyisobutene, 0.6 parts of citron fruit extract and 2 parts of peony seed oil.

[0021] Example 2 A soothing, firming and anti-wrinkle composition is obtained by mixing 7 parts of hydrogenated polyisobutene, 0.3 parts of citron fruit extract and 3 parts of peony seed oil.

[0022] Example 3 A soothing, firming and anti-wrinkle composition is obtained by mixing 10 parts of hydrogenated polyisobutene, 1 part of citron fruit extract and 0.1 part of peony seed oil.

[0023] Example 4 A soothing, firming and anti-wrinkle composition is obtained by mixing 5 parts of hydrogenated polyisobutene, 0.101 parts of citron fruit extract and 0.101 parts of peony seed oil.

[0024] Comparative Example 1 5.202 parts of hydrogenated polyisobutylene.

[0025] Comparative Example 2 5.202 parts citron fruit extract.

[0026] Comparative Example 3 5.202 parts of peony seed oil.

[0027] Comparative Example 4 A soothing, firming and anti-wrinkle composition is obtained by mixing 5.1 parts of hydrogenated polyisobutene and 0.102 parts of citron fruit extract.

[0028] Comparative Example 5 A soothing, firming and anti-wrinkle composition is obtained by mixing 5.1 parts of hydrogenated polyisobutene and 0.102 parts of peony seed oil.

[0029] Comparative Example 6 A soothing, firming and anti-wrinkle composition is obtained by mixing 2.601 parts of citron fruit extract and 2.601 parts of peony seed oil.

[0030] Comparative Example 7 A soothing, firming and anti-wrinkle composition is obtained by mixing 5 parts of jojoba seed oil, 0.101 parts of citron fruit extract and 0.101 parts of peony seed oil.

[0031] Efficacy testing 1. Soothing effect 1. Test purpose and principle Relevant literature shows that hyaluronic acid is widely present in the skin and other tissues, and its relationship with the skin is very close. Large molecular weight hyaluronic acid can inhibit inflammatory responses, thereby alleviating the discomfort caused by skin inflammation. Large molecular weight hyaluronic acid is degraded by hyaluronidase, resulting in a decrease in its content. This degradation product produces N-acetylglucosamine. Measuring the N-acetylglucosamine content in the reaction system can indirectly reflect the activity of hyaluronidase.

[0032] 2. Correlation of test methods This in vitro method is suitable for cosmetics claiming soothing properties. Relevant literature indicates a certain correlation between the trends observed in in vivo methods and this in vitro method. Through experimental design, the N-acetylglucosamine content in the reaction system was measured to determine the hyaluronidase inhibition rate of the sample group.

[0033] 3. Test methods 1) Weighing the sample; 2) pre-treating the sample set and then pre-treating it to prepare a mixed solution; 3) Add the reactants, mix well, and incubate in a water bath at 37°C; 4) Add pH adjuster, boil in water for 10 minutes, and cool to room temperature; 5) Measure the OD value on the test machine and calculate the hyaluronidase activity inhibition rate.

[0034] The present application commissioned a testing agency to test Examples 1-4 and Comparative Examples 1-7 according to the above method, and the results are shown in Table 1; Table 1 Hyaluronidase activity inhibition results of the compositions of Examples 1-4 and Comparative Examples 1-7 Hyaluronidase activity inhibition rate% Example 1 55.04 Example 2 65.57 Example 3 54.34 Example 4 60.26 Comparative Example 1 50.55 Comparative Example 2 19.87 Comparative Example 3 53.84 Comparative Example 4 42.84 Comparative Example 5 52.73 Comparative Example 6 46.9 Comparative Example 7 50.88

[0035] According to the data in Table 1: According to the data of Examples 1-4, the technical solution of the present application has an excellent soothing effect. Examples 1-4 still maintain a hyaluronidase activity inhibition rate of more than 54% under different changes in component dosage.

[0036] According to the data of comparative examples 1-3, the soothing effect ranking among hydrogenated polyisobutene, citron fruit extract and peony seed oil should be peony seed oil > hydrogenated polyisobutene > citron fruit extract, and peony seed oil and hydrogenated polyisobutene have better hyaluronidase activity inhibition rate.

[0037] At the same time, according to the comparison of the data of Comparative Examples 4-6, it can be seen that since the sample testing makes the compositions diluted to the same concentration, that is, when the total amount of the composition is consistent, the hyaluronidase activity inhibition rate of the compound of hydrogenated polyisobutene and citron fruit extract in Comparative Example 4 is between the hyaluronidase activity inhibition rates of hydrogenated polyisobutene and citron fruit extract itself; the hyaluronidase activity inhibition rate of the compound of hydrogenated polyisobutene and peony seed oil in Comparative Example 5 is between the hyaluronidase activity inhibition rates of hydrogenated polyisobutene and peony seed oil. The hyaluronidase activity inhibition rate of peony seed oil and citron fruit extract in comparative example 6 is between the hyaluronidase activity inhibition rates of peony seed oil and citron fruit extract itself; this shows that the combination of hydrogenated polyisobutene, citron fruit extract and peony seed oil does not produce a significant synergistic effect, but based on the hyaluronidase activity inhibition rate of citron fruit extract itself is only 19.87%, we can speculate based on the data of comparative examples 4-6: (1) There is no synergistic effect between the combination of citron fruit extract and hydrogenated polyisobutene. The lower concentration of citron fruit extract still has a certain effect on the composition of Comparative Example 4, so that the hyaluronidase activity inhibition rate of Comparative Example 4 is lower than that of Comparative Example 1; (2) Although the concentration of peony seed oil in comparative example 5 is low, it still has a certain increase in the inhibition rate of hyaluronidase activity compared with hydrogenated polyisobutene itself. The combination of peony seed oil and hydrogenated polyisobutene has no synergistic effect or a weak synergistic effect; (3) The combination of citron fruit extract and peony seed oil has a certain degree of synergistic effect. In Comparative Example 6, the concentrations of citron fruit extract and peony seed oil are 50% each. Theoretically, the hyaluronidase activity inhibition rate should be much lower than that of Comparative Example 3. However, the hyaluronidase activity inhibition rate of Comparative Example 6 reached 46.9%, which is even higher than that of Comparative Example 4. It is speculated that the combination of citron fruit extract and peony seed oil produces a certain synergistic effect, thereby improving the hyaluronidase activity inhibition rate of the composition.

[0038] However, from the comparison of the data of Example 4 and Comparative Examples 1-6, it can be seen that when hydrogenated polyisobutene, citron fruit extract and peony seed oil are compounded, the hyaluronidase activity inhibition rate exerted is significantly increased. Based on the above speculation, we have reason to believe that the compounding of hydrogenated polyisobutene, citron fruit extract and peony seed oil further enhances the synergistic effect of citron fruit extract and peony seed oil, thereby producing further synergistic effects and significantly improving the hyaluronidase activity inhibition rate of the composition.

[0039] Although Comparative Example 7 uses jojoba seed oil to replace hydrogenated polyisobutene and also has a higher hyaluronidase activity inhibition rate, its hyaluronidase activity inhibition rate is significantly lower than that of Example 4 of the present application.

[0040] 2. Antioxidant Effect 1. Test purpose and principle External and internal factors can lead to the generation of free radicals, which possess extremely strong oxidative potential. They attack any biomolecule through oxidative reactions, undergoing various oxidative reactions with macromolecules such as lipids, carbohydrates, proteins, and DNA, causing oxidative damage such as denaturation, crosslinking, and breakage. This in turn leads to disruption of cellular structure and function, as well as damage to tissues and organ pathology. This manifests itself on the skin as aging and wrinkles. When a free radical scavenger is present in a sample, the light absorption of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), a stable, long-lived free radical, decreases. This allows evaluation of the sample's free radical scavenging capacity, or, more accurately, its antioxidant activity.

[0041] 2. Correlation of test methods This in vitro method is suitable for cosmetics claiming anti-wrinkle efficacy. Relevant literature indicates that when a free radical scavenger is present in the sample group, the degree of discoloration of the DPPH solution is linearly correlated with the free radical scavenging ability of the free radical scavenger. This allows the free radical scavenging ability of the sample group to be evaluated, i.e., the antioxidant activity.

[0042] 3. Test methods 1) Weighing the sample; 2) Pre-treat the sample and prepare a solution for use; 3) Add the free radical DPPH, prepare multiple tubes of samples, and shake well for later use; 4) After standing at room temperature for a period of time, measure the absorbance value on the test machine; 5) Record the data and compare the DPPH radical scavenging rate of the product group with the sample group and the control group.

[0043] The present application commissioned a testing agency to test Examples 1-4 and Comparative Examples 1-7 according to the above method. The results are shown in Table 2. Table 2 DPPH radical scavenging rate results of the compositions of Examples 1-4 and Comparative Examples 1-7 DPPH free radical scavenging rate% Example 1 28.91 Example 2 66.29 Example 3 25.47 Example 4 61.31 Comparative Example 1 15.45 Comparative Example 2 5.48 Comparative Example 3 20.02 Comparative Example 4 2.14 Comparative Example 5 19.6 Comparative Example 6 5.46 Comparative Example 7 33.18

[0044] According to the results in Table 2, we can see that: According to the data of Examples 1-4, the technical solution of the present application has a certain antioxidant effect. However, unlike Table 1, the DPPH radical scavenging rates shown in Examples 1-4 of the present application are significantly different. Under specific component ratios, the DPPH radical scavenging rates shown in Examples 2 and 4 exceed 60%, while the DPPH radical scavenging rates shown in Examples 1 and 3 are only 25.47% and 28.91% respectively. It is speculated that the components of the composition need to be in a specific ratio to fully exert their antioxidant effect.

[0045] It should be noted that the composition has a strong antioxidant capacity and can effectively enhance the skin's firming and anti-wrinkle capabilities.

[0046] In order to further verify the reason for the large difference in antioxidant efficacy of Examples 1-4, the present application verified the DPPH radical scavenging rates of hydrogenated polyisobutene, citron fruit extract and peony seed oil itself through Comparative Examples 1-3, and found that the DPPH radical scavenging rate of citron fruit extract was only 5.48%. Combined with the component content analysis of Examples 1-4, it was speculated that: the high concentration of citron fruit extract in the composition will affect the DPPH radical scavenging rate of the composition, and Comparative Examples 4 and 6 also verified this; however, Comparative Example 4 only slightly increased the concentration of citron fruit extract in the absence of peony seed oil, but the DPPH radical scavenging rate it exhibited was sharply reduced compared to Example 4, which may be that not only the concentration of citron fruit extract has a significant impact on the antioxidant efficacy of the composition.

[0047] Therefore, according to the comparison of the data of Example 4 and Comparative Example 5, it can be seen that when the citron fruit extract is missing, the DPPH radical scavenging rate of the composition of Comparative Example 4 is not significantly improved. Therefore, it can be inferred that: in the technical solution of the present application, the combination of citron fruit extract, hydrogenated polyisobutene, and peony seed oil produces a synergistic effect of improving the DPPH radical scavenging rate of the composition, but excessively high concentrations of citron fruit extract will produce a counterproductive effect. That is to say, the synergistic effect of the composition can only be effectively exerted at a specific concentration of citron fruit extract.

[0048] Application Examples A lip wrinkle-reducing gel is prepared according to the formula in Table 3. The preparation method is as follows: 1. Add phase A to the emulsifying pot, stir and heat to 80℃, homogenize for 3 minutes, keep warm for 20 minutes, and start cooling; 2. Cool down to 55℃ and add phase B into the emulsifier, stirring for 10 minutes.

[0049] Table 3 Lip wrinkle reduction gel formula

[0050] Application Example Efficacy Test The following tests were commissioned by Guangdong Fangtu Testing Technology Co., Ltd. for the application examples: I. Human Efficacy Evaluation Tests (Moisturizing, Anti-Wrinkle, Firming, Repair) 1.1 Test Methods: ALS-SOP-LT-006 "Specifications for Human Moisturizing Efficacy Test", ALS-SOP-LT-032 "Specifications for Human Anti-Wrinkle Efficacy Test", ALS-SOP-LT-033 "Specifications for Human Firming Efficacy Test", ALS-SOP-LT-028 "Specifications for Human Repair Efficacy Test", ALS-SOP-IE-038 "Standard Operating Procedure for Skin 3D Imaging Analysis System Antera3D", ALS-SOP-IE-042 "Standard Operating Procedure for Skin Surface Texture Analysis System (Skin Microscope) Visioscan VC20 Plus" 1.2 Method Source: Refer to T / CAB 0152-2022 "Test Methods for Seven Efficacy of Cosmetics: Anti-Wrinkle, Firming, Moisturizing, Oil Control, Repair, Nourishing, Soothing" 1.3 Test Results, see Table 4; Table 4 Test Results of Human Efficacy Evaluation Test parameters Time point Average improvement rate Difference analysis (compared with before use) Parameter Explanation Stratum corneum moisture content After using the sample for 14 days 33.11% There is a significant difference An increase in the test value indicates an increase in the moisture content of the skin's stratum corneum. Lip wrinkle volume After using the sample for 14 days 7.70% There is a significant difference A decrease in the measurement value indicates a reduction in the volume of the lip lines. Average depth of lip lines After using the sample for 14 days 8.00% There is a significant difference A decrease in the measured value indicates a decrease in the average depth of lip wrinkles. Lip wrinkle length After using the sample for 14 days 19.42% There is a significant difference A decrease in the measured value indicates a decrease in the length of the lip lines. Number of lip lines After using the sample for 14 days 22.97% There is a significant difference A decrease in the measurement value indicates a decrease in the number of lip lines. Skin firmness R0 value After using the sample for 14 days 20.78% There is a significant difference A lower measurement indicates improved skin firmness. Skin elasticity R2 value After using the sample for 14 days 18.88% There is a significant difference An increase in the measured value indicates improved skin elasticity. Transepidermal water loss rate After using the sample for 14 days 24.43% There is a significant difference A decrease in the measured value indicates a decrease in the transepidermal water loss rate. SEsc value of skin scales After using the sample for 14 days 19.32% There is a significant difference A lower measurement indicates less scaling of the skin.

[0051] II. Acute Oral Toxicity Test Test Method: 1. Materials and Methods Detection Method: Refer to Chapter 6.2 Acute Oral Toxicity Test of "Technical Specifications for Cosmetics Safety" (2015 Edition).

[0052] I 2. Detection Environment: Barrier Environment Animal House, Experimental Animal Use License Number: SYXK (Guangdong) 2022-0198, Temperature: 20~26°C, Relative Humidity: 30~70%.

[0053] 3. Experimental Animals and Feed: 10 SPF-grade Kunming mice, 5 males and 5 females, female animals are not pregnant and have never given birth, body weight ￥19.5~20.9g, experimental animals are purchased from Guangzhou Ruige Biotechnology Co., Ltd., Experimental Animal Production License Number: SCXK (Guangdong) 2023-0059, Animal Quality Certificate Number: 44827200020619. Feed is purchased from Henan Huanyu Hekang Biotechnology Co., Ltd., Certificate Number: 4109782500033951. 4. Sample Preparation: Weigh 5.00g of the sample, add corn oil to make the volume up to 20mL, and prepare a test solution with a concentration of 0.25g / mL.

[0054] 5. Test Method: a) Dosage: The formal trial adopted the single-dose limiting method, and the experimental design was designed to test the substance at a dose of 5000 mg / kg body weight; b) Exposure: Prior to the experiment, the animals were fasted overnight with unlimited access to water. During the formal experiment, the animals were weighed and administered the test solution via oral gavage at a volume of 0.2 mL / 10 g body weight. Three to four hours after exposure, the animals were fed a normal diet. c) Animal Observation: After exposure, each animal was observed for signs of poisoning and death for 14 consecutive days. The animals were weighed on the 7th and 14th days after gavage. At the end of the observation period, the animals were sacrificed and autopsied.

[0055] 6. Evaluation criteria, see Table 5; Table 5 Oral toxicity classification <![CDATA[LD 50 (mg / kg)]]> Toxicity classification <50 Highly toxic 50~500 Moderately toxic 501~5000 Low toxicity >5000 Practically non-toxic

[0056] 7. Experimental Results 1) Weight changes, see Table 6; 2) Death: No mice died during the experiment, and the mortality rate was 0%, see Table 6; 3) Toxicity observation: No toxicity symptoms were observed in the experimental mice; 4) Gross pathological examination: No abnormalities were found in the tissues and organs of all surviving mice.

[0057] Table 6 Body weight changes of experimental mice after oral gavage

[0058] The test substance is a practically non-toxic substance.

[0059] The embodiments presented herein are merely embodiments selected from a combination of all possible embodiments. The appended claims should not be limited by the embodiments described herein. Some numerical ranges used in the claims include subranges therein, and variations in these ranges should also be covered by the appended claims.

Claims

1. A soothing, firming and anti-wrinkle composition, characterized in that: The invention comprises, by weight, 5-15 parts of hydrogenated polyisobutene, 0.1-1 parts of citron fruit extract and 0.1-3 parts of peony seed oil.

2. Use of the soothing, firming and anti-wrinkle composition according to claim 1 in preparing skin care products.

3. A gel for lightening lip lines, characterized in that: Contains 0.1-99 wt % of the soothing, firming and anti-wrinkle composition according to claim 1 or 2.

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