Crosslinking hyaluronic acid gel resisting hydrolysis of hyaluronidase
A technology for cross-linking hyaluronic acid and hyaluronidase, applied in the field of materials, can solve problems such as adverse reactions, and achieve the effects of large application potential, large economic benefits, and enhanced maintenance time and plasticity.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0037] Example one:
[0038] The names of natural flavin coenzymes and their derivatives are: including riboflavin: Riboflavin, isoalloxazine: isoalloxazine,
[0039] 7-methyl-8-chloro-10-(1′-D-ribityl)isoalloxazine(7-M-8-C),
[0040] 7-bromo-8-methyl-10-(1′-D-ribityl)isoalloxazine(7-B-8-M),
[0041] 7-methyl-8-bromo-10-(1′-D-ribityl)isoalloxazine(7-M-8-B),
[0042] 7-chloro-8-ethyl-10-(1′-D-ribityl)isoalloxazine(7-C-8-E),
[0043] 7,8-diethyl-10-(1′-D-ribityl)isoalloxazine(7,8-D).
Example Embodiment
[0045] Embodiment two:
[0046] Inhibition of human-derived recombinant hyaluronidase activity using natural flavin coenzyme and its derivatives
[0047] Establishment of reaction system:
[0048] 0.5g cross-linked hyaluronic acid gel, hyaluronic acid content is 28mg / g, add 2mL PBS buffer, pH 6.5-7.5, which contains human-derived recombinant hyaluronidase 250U / mL; in addition, add a certain amount of Natural flavin coenzyme or its derivative molecules. The entire reaction system was kept in a 37°C water bath for 72 hours, and samples were taken every 24 hours. After centrifugation at 12000 rpm for 10 minutes, the supernatant was removed to determine the free hyaluronic acid content.
[0049] (1) Determination of the optimal addition amount of FAD
[0050] Determine the maximum amount of ligand molecules added. In the above reaction system, add 0, 0.2, 0.4, 0.6, 0.8, 1.0 mmol / L of FAD, in a 37℃ water bath for 72 hours, sampling every 24 hours, centrifuge at 12000rpm for 10 minutes, re...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap