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Uric acid detection method based on silver triangular nanoflakes

A detection method and technology of triangular slices, which are used in the measurement of color/spectral characteristics and analysis by chemical reaction of materials, can solve the problems of complicated and time-consuming pretreatment, increased detection costs, and large damage to chromatographic columns, and achieve synthesis Fast and simple method, easy access, high sensitivity and selective effect

Inactive Publication Date: 2019-01-25
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem with this method is the use of expensive uricase, which leads to an increase in detection costs
Although high-performance liquid chromatography has the characteristics of simple and fast operation, the pretreatment of samples is complicated and time-consuming, and most of the mobile phases use phosphate, which greatly damages the chromatographic column.

Method used

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  • Uric acid detection method based on silver triangular nanoflakes
  • Uric acid detection method based on silver triangular nanoflakes
  • Uric acid detection method based on silver triangular nanoflakes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Add a certain amount of ultrapure water (24mL), AgNO 3 The solution (1mL, 5mM), trisodium citrate solution (500μL, 100mM), hydrogen peroxide (60μL, 30wt.%) were added to the round bottom flask and stirred vigorously for 2min, then quickly added freshly prepared NaBH in ice water 4 (140μL, 100mM) continue to stir for 25min to obtain the precursor sol; with NaBH 4 Adding, transfer the synthesized precursor sol to the beaker, wrap the outer wall of the beaker with aluminum foil and place it under a 520nm LED light for 48 hours to obtain blue-violet silver nanosheets; the final silver nanotriangular sheet (Ag NPrs) Packed in a brown bottle and stored at 4℃, then characterize the silver nano triangle sheet; figure 2 As shown, the absorption peak is the characteristic absorption peak of the silver nano-triangular sheet, and the maximum absorption peak is at 575nm, as shown by the transmission electron microscope image ( image 3 ) It can be seen that the shape of the synthesized...

Embodiment 2

[0035] Add a certain amount of ultrapure water, AgNO 3 The solution, trisodium citrate solution, and hydrogen peroxide were added to the round bottom flask and stirred vigorously for 1 min. Among them, ultrapure water: AgNO 3 Solution: trisodium citrate solution: the ratio of hydrogen peroxide (L / mmol / mmol / mL) is 20:8:45:65, and the mass concentration of hydrogen peroxide is 25%; then quickly add freshly prepared NaBH in ice water 4 Continue to stir for 30min, NaBH 4 With AgNO 3 The molar ratio of the solution is 6:25, and the precursor sol is obtained; with NaBH 4 Adding, transfer the synthesized precursor sol to the beaker, wrap the outer wall of the beaker with aluminum foil and place it under a 520nm LED light for 48 hours to obtain blue-violet silver nanosheets; the final silver nanotriangular sheet (Ag NPrs) Packed in a brown bottle and stored at 4℃, then characterize the silver nano triangle sheet; figure 2 As shown, the absorption peak is the characteristic absorption pea...

Embodiment 3

[0037] Add a certain amount of ultrapure water, AgNO 3 The solution, trisodium citrate solution, and hydrogen peroxide were added to the round-bottom flask and stirred vigorously for 3 minutes. Among them, ultrapure water: AgNO 3 Solution: trisodium citrate solution: the ratio of hydrogen peroxide (L / mmol / mmol / mL) is 28:3:55:55, and the mass concentration of hydrogen peroxide is 35%; then quickly add freshly prepared NaBH in ice water 4 Continue stirring for 20min, NaBH 4 With AgNO 3 The molar ratio of the solution is 8:25, and the precursor sol is obtained; with NaBH 4 Adding, transfer the synthesized precursor sol to the beaker, wrap the outer wall of the beaker with aluminum foil and place it under a 520nm LED light for 48 hours to obtain blue-violet silver nanosheets; the final silver nanotriangular sheet (Ag NPrs) Packed in a brown bottle and stored at 4℃, then characterize the silver nano triangle sheet; figure 2 As shown, the absorption peak is the characteristic absorptio...

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Abstract

The invention discloses a uric acid detection method based on silver triangular nanoflakes. The uric acid detection method comprises the following steps that the silver triangular nanoflakes are prepared; a to-be-detected sample is added into the silver triangular nanoflakes, then potassium rhodanide is added, and at this moment, if the absorption peak at 400 nm is weakened and the absorption peakat 575 nm rises again, it is expressed that the to-be-detected sample contains uric acid. The detection method is high in flexibility and selectivity.

Description

Technical field [0001] The invention belongs to the technical field of uric acid detection, and specifically relates to a method for detecting uric acid based on silver nano triangular tablets. Background technique [0002] Under normal circumstances, there is about 1200 mg of uric acid in the body, about 600 mg is newly produced every day, and 600 mg is excreted at the same time, in a balanced state. However, if the body produces too much to be excreted or the uric acid excretion mechanism is degraded, the body will retain too much uric acid. When the blood uric acid concentration is greater than 7 mg / dL, the body fluid will become acidic and affect the normal function of human cells. Long-term neglect will cause gout. In addition, too much fatigue or lack of rest can also lead to relatively slow metabolism and cause the onset of gout. [0003] The detection of uric acid is helpful for the early diagnosis of related diseases. The current detection methods for uric acid are main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75G01N21/33
CPCG01N21/33G01N21/75
Inventor 凌剑谭远航刘安勇
Owner YUNNAN UNIV
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