Paper-based device based on moving valve and molecular imprinting technology, and manufacturing method and application thereof

A technology of molecular imprinting technology and production method, which is applied in the field of paper-based devices, can solve the problems of strict storage conditions, cumbersome washing process, and long reaction time, and achieve the effects of reducing washing procedures, reducing costs, and simple operation

Active Publication Date: 2019-10-29
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has disadvantages such as high antibody cost, strict storage conditions, long reaction time, and cumbersome washing process.

Method used

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  • Paper-based device based on moving valve and molecular imprinting technology, and manufacturing method and application thereof
  • Paper-based device based on moving valve and molecular imprinting technology, and manufacturing method and application thereof
  • Paper-based device based on moving valve and molecular imprinting technology, and manufacturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for manufacturing a paper-based device based on a moving valve and molecular imprinting technology, comprising the following steps:

[0040] (1.1) Pattern design: use Adobe illustrator drawing software to design 4 kinds of chip patterns (see figure 1 ), which are respectively a working electrode part, four counter / reference electrode parts, a washing liquid recovery part, and a moving valve.

[0041] And requires the working electrode part (see figure 2 ) is 50mm×25mm in size, has two circular sample pools with a diameter of 9mm, a material addition pool with a diameter of 5mm, two disconnected channels pointing to the two sample pools with a width of 2mm, and three rivet holes; washing liquid The size of the recovery part is 40mm×50mm, connected with the working electrode part, and has two washing and drainage hydrophilic channels; the counter / reference electrode part is circular, with a diameter of 24mm, and the center position has a hydrophilic area of ​​t...

Embodiment 2

[0049] A paper-based device based on a moving valve and molecular imprinting technology prepared in Example 1 is used for qualitative and quantitative analysis of cancer precursor antigen tumor markers in human serum, specifically including the following steps:

[0050] (2.1) Select cancer blank antigen tumor markers according to the type of tumor that needs to be diagnosed, and make a paper-based device based on moving valve and molecular imprinting technology that can detect biomarkers;

[0051] (2.2) First, close the counter / reference electrode part, the moving valve, and the washing liquid recovery part, so that they are away from the working electrode part. Add 5 μL serum sample dropwise to the sample cell of the working electrode part, and after equilibrating at room temperature for 10 minutes, fold the washing liquid recovery part to fit the working electrode part, and wash the sample cell with 10 mM, pH=7.4 PBS buffer solution 3 over, to remove interfering substances; ...

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Abstract

The invention provides a paper-based device based on a moving valve and molecular imprinting technology, and a manufacturing method and application thereof. The manufacturing method comprises the following steps of pattern design, chip fabrication, electrode construction on a paper base, chip connection, synthesis of a functionalized molecularly imprinted polymer layer, electrochemical polymerization, and elution of template molecules. The device is used for analysis and detection of biomarkers in serum samples. The invention provides a paper-based device based on moving valve and molecular imprinting technology, a manufacturing method and application thereof, and provide a novel device for clinically detecting biomarkers. The device is used for in-situ synthesis of biomarker molecularlyimprinted polymer, forms a clinical application method, creates a new strategy for antibody-free biomarker analysis, realizes the entire process from device fabrication to test result output on the paper-based device, and is economical, portable, high in speed.

Description

technical field [0001] The invention belongs to the technical field of electrical analysis and medical diagnosis, and relates to the application of microfluidic chip technology, in particular to a paper-based device based on moving valve and molecular imprinting technology, its manufacturing method and application. Background technique [0002] Human biomarker analysis is an effective medical diagnostic method. With the development of biological science, more and more biomarkers have been discovered and used to diagnose human diseases. Among them, the analysis of tumor markers has been widely used in the diagnosis of tumor incidence, but due to untimely diagnosis, the best time for treatment is often delayed. Therefore, it is of great significance to provide an instrument with the characteristics of less sample requirements, low cost, and convenient use for the analysis of tumor markers. [0003] In addition, higher sensitivity, higher informativeness, and low-cost analytic...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/53G01N35/00G01N27/48B01L3/00
CPCG01N33/57484G01N33/5302G01N35/00G01N27/48B01L3/5027G01N2035/00108G01N2035/00247
Inventor 李博伟齐骥陈令新李博峰罗立强周俊瑞
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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