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Zn/H2aip fluorescent probe, preparation and application of Zn/H2aip fluorescent probe in detection of tetracycline antibiotics

A fluorescent probe, tetracycline technology, used in the application field of identifying tetracycline antibiotics by fluorescence integration

Pending Publication Date: 2021-07-23
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For all the above reported MOFs sensors, there is no specific distinction between multiple analytes of the same family, which is the most challenging part of designing sensors

Method used

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  • Zn/H2aip fluorescent probe, preparation and application of Zn/H2aip fluorescent probe in detection of tetracycline antibiotics
  • Zn/H2aip fluorescent probe, preparation and application of Zn/H2aip fluorescent probe in detection of tetracycline antibiotics
  • Zn/H2aip fluorescent probe, preparation and application of Zn/H2aip fluorescent probe in detection of tetracycline antibiotics

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Synthesis of fluorescent probes

[0030] Take ZnO (0.05g), 5-aminoisophthalic acid (0.1g) and 8mL of DMF, treat the mixture with ultrasonic, and put it in a reaction kettle at 120°C for 48 hours. After taking it out, it was washed successively with DMF and absolute ethanol, dried in a vacuum oven at 60 °C and then ground to obtain Zn / H 2 aip fluorescent probe. The probe material is prepared as a 1mg / mL stock solution, which can be stored in the refrigerator for future use.

[0031] (2) Qualitative and quantitative detection of TCs

[0032] Probe Zn / H of the present invention 2 The fluorescence response of aip to TCs was carried out in Tris-HCl buffer (10 mM, pH=8). 100 μL Probe Zn / H 2 aip (1mg / mL) was added to a cuvette containing 2mL of buffer, and it was incubated for 30 minutes. The final concentration of the probe was 0.05mg / mL, and then TCs were added to the above solution. The final concentrations of TCs were 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24...

Embodiment 2

[0040] The doxycycline sample solution was prepared and placed in 2 mL of Tris-HCl buffer solution (10 mM, pH=8) with a final concentration of 4 μM. The integral area method of the present invention is adopted for detection. First 100 μL probe Zn / H 2 aip (1mg / mL) was added to the cuvette of the above-mentioned 2mL Tris-HCl buffer, and incubated for 30 minutes. The final concentration of the probe was 0.05mg / mL, and the fluorescence detection was carried out with 365nm as the excitation wavelength. The fluorescence spectrum was from 385nm is recorded to 700nm, and the area under the curve between 436nm and 700nm is integrated to obtain the area integral value, and then according to image 3 The readings of the four standard curves are calculated to obtain a concentration range of 4.01-12.12 μM in the TCs sample solution. Through the application "TCs Analysis", the interface can directly display "TCs contamination degree: 4.01-12.12μM".

Embodiment 3

[0042] The doxycycline sample solution was prepared so that it was placed in 2 mL Tris-HCl buffer solution with a final concentration of 8 μM. The method described in Example 2 was used for detection, and the concentration of the TCs sample solution was obtained to be 8.05-21.4 μM. The application "TCs Analysis" can directly display "TCs contamination degree: 8.05-21.4μM" on the interface.

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Abstract

The invention relates to a metal organic framework (MOFs) fluorescent probe for detecting tetracycline antibiotics (TCs) and a preparation method of the metal organic framework (MOFs) fluorescent probe, and also provides a fluorescence integration method and an application program for designing, analyzing and recognizing the overall pollution degree of the tetracycline antibiotics. According to the fluorescent probe, 5-aminoisophthalic acid (H2aip) is used as an organic ligand of a stable three-dimensional framework, transition metal Zn (II) is used as a metal source, and the fluorescent probe with unique response to TCs is synthesized. Meanwhile, based on a fluorescence integration method, the method is not limited to identification of a single antibiotic, the pollution degree of the TCs can be quantified through a TCs Analysis application program, through precise data processing and intelligent analysis, a new method is provided for measuring the overall pollution degree of the TCs, and the method is suitable for portable analysis and detection.

Description

technical field [0001] The invention belongs to the technical field of antibiotic detection and analysis, and in particular relates to the synthesis of a fluorescent probe for recognizing tetracyclines (TCs), the proposal of a fluorescence integration method, and an application program for identifying tetracycline antibiotics by using the fluorescence integration method. Background technique [0002] Metal-organic frameworks (MOFs) are composed of ordered combinations of organic ligands and metal ions, also known as porous coordination polymers. Due to the large internal specific surface area, multi-topological network structure, low density, uniform cavity, permanent porosity, acid resistance, alkali resistance, and high thermal stability of MOFs, it has become a new type of supramolecular research material that has been widely explored. Gas separation, catalysis, drug delivery, optical and electronic sensing and other fields of application, and 5-aminoisophthalic acid is a...

Claims

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Application Information

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IPC IPC(8): C09K11/06C08G83/00G01N21/64
CPCC09K11/06C08G83/008G01N21/643C09K2211/188Y02A50/30
Inventor 王素华陈宏霞彭俊翔余龙孙明泰
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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