4 results about "Myostatin Gene" patented technology

A composition for modifying a myostatin gene, the composition comprising: a guide RNA; a Cas protein; wherein the target sequence is one selected from: SEQ ID NO: 38-60 and the guide sequence is one s

This invention discloses a targeted MSTN The sgRNA of the gene and its application in constructing a feline myostatin gene knockout model; the targeted MSTN The nucleotide sequence of the gene's sgRNA is shown in SEQ ID NO.1 and / or SEQ ID NO.2. This invention systematically establishes a CRISPR / Cas9 genome editing platform suitable for domestic cats for the first time and successfully constructs the world's first... MSTN The gene knockout live model in domestic cats represents a breakthrough in the technical barriers to functional gene research in domestic cats. It has been demonstrated that sgRNA is a highly efficient editing tool for the MSTN gene in domestic cats. The constructed... MSTN The gene knockout domestic cat model provides an unprecedented tool for in-depth research on muscle biology and muscle diseases in mammalian (cat) systems that are more closely related to humans. It fills the gap in large animal models in this field and has significant value for basic scientific research and translational medicine.

We provide an animal having a myostatin gene in which 12 base pairs of exon 2 are deleted. [Solution] A transgenic animal is provided having an artificially modified myostatin gene in its genome, wherein the artificial modification is located in the second exon of the myostatin gene, and the artificial modification is a deletion of 12 base pairs in the second exon that encodes an amino acid sequence in the order of leucine, tryptophan, isoleucine, and tyrosine, compared to the amino acid sequence of myostatin in wild-type animals, and the transgenic animal expresses less myostatin gene mRNA than wild-type animals and expresses a mature myostatin protein with the same sequence as wild-type animals.

An antisense oligonucleotide of 15-30 bases or a salt or a solvate thereof, wherein the antisense oligonucleotide has a nucleotide sequence complementary to a target site in exon 1 of the myostatin gene and is capable of inhibiting the production of the mRNA of the myostatin gene. A pharmaceutical drug, a food, a feed, an agent for promoting myocyte proliferation and / or hypertrophy, an agent for increasing muscle mass and / or inhibiting muscle weakness, an agent for inhibiting production of the mRNA of the myostatin gene, and an inhibitor of the function of myostatin, each of which comprises the above antisense oligonucleotide or a salt or a solvate thereof.