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Continuous enlarging cultivation device and method for mineral-bioleaching microorganisms

A technology for leaching microorganisms and expanding culture, which is applied in the field of continuous expanding culture devices for leaching microorganisms, can solve problems such as difficulty in chemical culture, and achieve the effects of convenient inoculation and expansion, compact structure and high culture efficiency.

Active Publication Date: 2018-04-06
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the deficiencies and defects mentioned in the above background technology, and to provide a method for maintaining the continuous production of high-concentration bacterial liquid while effectively alleviating the problem of difficult industrial cultivation of existing ore leaching microorganisms. Continuous expansion culture device and culture method for ore leaching microorganisms with strain loss in reactor

Method used

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  • Continuous enlarging cultivation device and method for mineral-bioleaching microorganisms
  • Continuous enlarging cultivation device and method for mineral-bioleaching microorganisms
  • Continuous enlarging cultivation device and method for mineral-bioleaching microorganisms

Examples

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Effect test

Embodiment 1

[0056] A kind of ore leaching microorganism continuous expansion culture device of the present invention, such as Figure 1-3 As shown, it includes cylinder 1, which is sequentially composed of an upper cylinder that can collect overflow discharge liquid, a middle cylinder that can continuously expand and cultivate microorganisms, and a lower cylinder 14 that can collect and discharge sediment. The top of the body is provided with a top cover 12, and the top cover 12 is provided with a top cover air outlet 13 and an observation window to ensure proper isolation of the microbial cultivation process from the outside world. The bottom side wall of the upper cylinder is provided with a feed liquid outlet 11; The top of the cylinder is provided with a sawtooth overflow weir device 10, which has the function of maintaining the liquid layer on the plate and making the liquid overflow evenly. The overflow weir device 10 is hollow embedded in the bottom of the upper cylinder; The botto...

Embodiment 2

[0061] A method for cultivating Thiobacillus ferrooxidans ( Acidithiobacillus ferrooxidans ATCC 23270, strain collection number: ATCC 23270, purchased from American Type Culture Collection), acidophilic heterotrophic bacteria ( Acidophilium acidophilum ATCC 27807, strain preservation number: ATCC 23270, purchased from American Type Microorganism Culture Collection), culture solution composition: (NH 4 ) 2 SO 4 , 2.5 g / L; MgSO 4 ·7H 2 O, 0.6g / L; K 2 HPO 4, 0.5 g / L; KCl, 0.1 g / L; Ca(NO 3 ) 2 , 0.01g / L; FeSO 4 ·7H 2 O, 50g / L.

[0062] The concrete operation of cultivating method comprises the following steps:

[0063] (1) Add the microbial culture solution through the feed port and the nutrient solution distribution plate, make it submerge the heating device and reach the bottom of the bottom packing assembly, adjust the pH to 1.5 by adjusting the acidity of the feeding medium, and set the culture temperature through the heating device 35°C, then uncover the top co...

Embodiment 3

[0070] A kind of cultivating and cultivating Thiobacillus ferrooxidans ( Acidithiobacillus ferrooxidans ATCC 23270, strain collection number: ATCC 23270, purchased from the American Type Microorganisms Collection), Leptohelicobacter ferrophilum ( Leptospirillum ferriphilum CS13, strain preservation number: M2015010, preservation place: China Center for Type Culture Collection), Sulfobacillus acidophilus ( Sulfobacillus acidophilus CS5, strain preservation number: M2015006, preservation location: China Center for Type Culture Collection). Composition of culture medium Composition of culture medium: (NH 4 ) 2 SO 4 , 2.5 g / L; MgSO 4 ·7H 2 O, 0.6g / L; K 2 HPO 4 , 0.5 g / L; KCl, 0.1 g / L; Ca(NO 3 ) 2 , 0.01g / L; FeSO 4 ·7H 2 O, 50g / L.

[0071] The concrete operation of cultivating method comprises the following steps:

[0072] (1) Add microbial culture solution through the feeding port and nutrient solution distribution plate, make it submerged in the heating device...

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Abstract

The invention discloses a continuous enlarging cultivation device for mineral-bioleaching microorganisms. The cultivation device comprises a cylinder which is formed by sequentially connecting an upper cylinder body, a middle cylinder body and a lower cylinder body, wherein the upper cylinder body can collect feed liquid which is discharged due to overflowing, the middle cylinder body can continuously cultivate the microorganisms in an enlarged mode, and the lower cylinder body can collect discharged precipitates. A detachable multi-layer filler assembly is arranged in the middle cylinder body, the multi-layer filler assembly is composed of a multi-layer filler box and filler which is arranged in the filler box, a heater, a nutrient solution distributing plate and an aeration device are arranged below the multi-layer filler assembly, and the aeration device is arranged below the nutrient solution distributing plate. The cultivation device is simple in design, continuous cultivation canbe achieved, the concentration of the microorganisms in a mature bacteria solution can be improved, and while continuous production of the high-concentration bacteria solution is maintained, the problem about the loss of strains in a reactor is effectively eased. The cultivation efficiency of the mineral-bioleaching microorganisms is improved, and the device has the advantages of being safe, efficient, low in space occupation, low in power consumption, simple and easy in operation and capable of achieving combined cultivation of a plurality of cultivation devices.

Description

technical field [0001] The invention belongs to the technical field of biometallurgy, and in particular relates to an ore leaching microorganism continuous expansion culture device and a culture method thereof. Background technique [0002] Biometallurgy technology is an interdisciplinary subject based on hydrometallurgy and microbiology. It is the fastest-growing new technology in the field of dressing and metallurgy. It has the advantages of processing low-grade complex ores, mild reaction, economical safety, low-carbon environmental protection, etc. It has broad industrial application prospects. This technology uses the extremely acidophilic iron-sulfur oxidizing bacteria widely present in the mining area to interact with the sulfide minerals in the ore to dissolve the minerals, and the valuable metal elements remain in the leaching slag or enter the solution in the form of ions, and then through enrichment, separation and Extraction and other processes have become comme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/04C12N1/20C22B3/18C12R1/01
CPCC12M23/02C12M23/22C12M23/38C12M23/44C12M25/20C12M29/06C12M41/12C12N1/20C22B3/18Y02P10/20
Inventor 周洪波周文博程海娜刘荣辉彭晶薛璟婷陈静葛杨田壮
Owner CENT SOUTH UNIV
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