11 results about "Microbiological Processes" patented technology

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.

The invention provides a method for establishing a soil organic carbon decomposition model based on a microbial process, which comprises the following steps: collecting soil and model parameters, and defining a model dry and wet region carbon library and microbial physiological state activity conditions; calculating the conversion speed of the carbon library between the dry and wet areas caused by the change of the water content in the soil; calculating the speed of forming DOC by enzyme catalytic decomposition of POC in the dry and wet regions and the speed of absorbing DOC by active microorganisms in the wet regions; calculating the speed of extracellular enzyme production, growth, respiration and death of the microorganisms and the MAOC density of the wet region during balance; establishing a mass balance equation of an enzyme carbon library ENZ and soil particle organic carbon POC; establishing a mass balance equation of dissolved organic carbon (DOC) and mineral bound organic carbon (MAOC); and establishing a dry and wet region microbial biomass MBC carbon library mass balance equation. According to the invention, the simulation capability of the model on organic carbon decomposed by soil microorganisms, especially pulse type soil respiration change caused by dry-wet change, is improved.

The present disclosure provides a microbiological process for the production of hydroquinone from benzoquinone in a microalgal system. The method is particularly characterized by the extraction of the hydroquinone product at a specific points in the process followed by re- introduction of the benzoquinone substrate into the microalgal system. The said method of the 0 present disclosure allows sustained production of hydroquinone.

The invention discloses a soil N2O emission estimation method coupled with a microorganism explicit simulation method and an implicit simulation method, and the soil N2O emission estimation method coupled with the microorganism explicit simulation method and the implicit simulation method comprises the following steps: S1, aiming at a research area, estimating a process of nitrifying NH4 < + > into NO2 <-> by using a microorganism implicit method; s2, estimating the biomass of the nitrifying bacteria participating in the nitrification process in the soil based on the nitrification NH4, n calculated in the S1; s3, estimating the denitrification process of the nitrifying bacteria by using a microbial explicit method, wherein the process comprises the following steps: estimating the process of denitrifying NO2 <-> into NO; s4, further estimating the process of denitrifying NO into N2O; and S5, calculating the release amount of N2O based on the steps S1 to S4. According to the method, the problems that implicit simulation lacks consideration on a microbial process and an initial value of explicit simulation is difficult to estimate can be effectively avoided, and the simulation precision of soil N2O emission can be effectively improved.

The present invention relates to a biotechnological process for the production of a fermented pollen comprising the inoculation of pollen with at least one lactic bacterium of the species Lactobacillus kunkeei selected from Lactobacillus kunkeei PF12 (DSM 32843), PF13 (DSM 32845) and / or PL13 (DSM 32844) and the fermentation of the pollen inoculated with said lactic bacterium. The fermented pollen obtained has nutritional and organoleptic properties similar to bee bread naturally produced inside the honeycomb of the beehive and finds application in the food and nutraceutical field. The invention further encompasses Lactobacillus kunkeei PF12 (DSM 32843), PF13 (DSM 32845) and / or PL13 (DSM 32844) as such and compositions comprising these strains.

Disclosed is a soil N2O emission estimation method that couples microbial explicit simulation and implicit simulation methods, comprising S1: for the study area, using the microbial implicit method to estimate the N2O emission from NH4 + Nitrification to NO2 ‑ S2: Based on the nitrification NH calculated in S1 4，n , estimate the biomass of nitrifying bacteria involved in the nitrification process in the soil; S3: use microbial explicit method to estimate the denitrification process of nitrifying bacteria, which includes: estimating NO2 ‑ The process of denitrification to NO; S4: further estimating the process of denitrification of NO to N2O; and S5: calculating the amount of N2O released based on S1-S4. This method effectively circumvents the problems of implicit simulation's lack of consideration of microbial processes and the difficulty in estimating initial values ​​in explicit simulation, effectively improving the simulation accuracy of soil N2O emissions.

The present invention relates to a method for decreasing microbial biomass and biodegradation rates of in situ bioremediation system using acetylene as the inhibitor.

A modular system for treating septic tank wastewater using substrates that support multiple biohabitats is lowered into a septic system where untreated wastewater enters the system from an opening defined by the lower region, travels upwardly through a bioremediation chamber containing a substrate oriented to form at least one vertical path, and treated wastewater exits through an opening defined by the upper region. An aerator positioned below the lower opening sends a stream of bubbles upwardly, thereby urging untreated wastewater upwardly into the bioremediation chamber. A mixer positioned below the lower opening intermittently emits large air bubbles that dislodge biomasses that accumulate on the substrate. A variety of microbiological processes occur on the surfaces and within the recesses of the substrate.

The utility model discloses a biochemical incubator which comprises an outer box body, one side of one end of the outer box body is movably hinged with an outer box door, one end in the outer box body is fixedly connected with an end plate, the center line of the end plate and the center line of the outer box body are on the same vertical plane, one side of the outer box body is fixedly connected with a control panel, and the control panel is fixedly connected with the outer box body. An isolation structure used for isolating the cultivation space is arranged in the outer box body. According to the biochemical incubator, through the arrangement of the partition plate, after the movable baffle is pressed to be separated from one end of the positioning piece in the microorganism cultivation process, the inner box door can be pulled to be opened, so that a culture dish is conveniently placed at the top end of the placing plate in the outer box body, and then the inner box door is closed; the movable baffle is ejected out by the spring and attached to one end of the positioning piece so that the inner box door can be positioned through the positioning piece, the interior of the outer box body can be divided into a plurality of spaces through the multiple sets of partition plates in the outer box body so that separated cultivation can be facilitated, and the problem that the cultivation space cannot be effectively isolated is solved.

The present invention provides a method for establishing a soil organic carbon decomposition model based on microbial processes, including: collecting soil and model parameters, defining the carbon pool in the dry and wet areas of the model and the physiological state and activity of microorganisms; calculating the speed of carbon pool conversion between dry and wet areas caused by changes in soil water content; calculating the speed of enzyme-catalyzed decomposition of POC to form DOC in the dry and wet areas, and the speed of active microorganisms absorbing DOC in the wet area; calculating the speed of microbial production of extracellular enzymes, growth, respiration and death, and the density of MAOC in the wet area at equilibrium; establishing a mass balance equation of enzyme carbon pool ENZ and soil particle organic carbon POC; establishing a mass balance equation of dissolved organic carbon DOC and mineral-bound organic carbon MAOC; and establishing a mass balance equation of microbial biomass MBC carbon pool in dry and wet areas. The present invention improves the model's ability to simulate the decomposition of organic carbon by soil microorganisms, especially the pulsed soil respiration changes caused by dry and wet changes.