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Conditioning biomass for microbial growth

A technology of biomass and microorganisms, which is applied in the fields of preparing the following compositions, cultivating the following microorganisms, and preparing products, and can solve problems such as unexplored uses

Inactive Publication Date: 2009-11-04
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] Although much research has focused on the detoxification of wood hydrolysates for ethanol fermentation, the use of lignocellulosic biomass as feedstock for other biotechnological processes is relatively unexplored

Method used

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  • Conditioning biomass for microbial growth

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Embodiment Construction

[0019] The present invention focuses on the problem of inhibitory substances in lignocellulosic biomass, which can adversely affect the performance of microbial cultures that use lignocellulosic biomass as a nutrient source. The present invention relates to a method for improving the utilization of lignocellulosic biomass as a source of nutrients for the growth of microorganisms in a variety of processes.

[0020] The present invention relates to the treatment or regulation of lignocellulose biomass as defined below with an enzyme composition comprising one or more phenol oxidizing enzymes. Without intending to be bound by any theory or mechanism, some inhibitors are phenolic compounds released during the decomposition of lignin, and it is believed that the phenol oxidase of the present invention catalyzes the oxidative coupling of such phenolic compounds to form insoluble polymers . The use of phenol oxidase in the present invention is different from using phenol oxidase as an an...

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Abstract

The present invention relates to methods for improving the yield of microbial processes that use lignocellulose biomass as a nutrient source. The methods comprise conditioning a composition comprisinglignocellulose biomass with an enzyme composition that comprises a phenol oxidizing enzyme. The conditioned composition can support a higher rate of growth of microorganisms in a process. In one embodiment, a laccase composition is used to condition lignocellulose biomass derived from non-woody plants, such as corn and sugar cane. The invention also encompasses methods for culturing microorganisms that are sensitive to inhibitory compounds in lignocellulose biomass. The invention further provides methods of making a product by culturing the production microorganisms in conditioned lignocellulose biomass.

Description

[0001] 1. Cross-reference with related applications [0002] This application claims the rights and priority of U.S. Provisional Application Serial No. 60 / 878,616, entitled "Conditioning Biomass for Microbial Growth", filed on January 3, 2007. The application is hereby incorporated in its entirety as a reference. [0003] 2. Government support [0004] Part of this work is based on the main contract number No.DE-AC36-99GO 10337 with the U.S. Department of Energy (USDepartment of Energy) and the sub-contract number No.ZCO-30017-01 under the National Renewable Energy Laboratory (National Renewable Energy Laboratory). Energy Laboratory) provided funding. Therefore, the U.S. government has certain rights in this invention. [0005] 3. Introduction [0006] The present invention relates to a method for improving the yield of a microbial process using lignocellulosic biomass as a nutrient source. 4. Background of the invention [0007] In recent years, considerable progress has been made...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/22C12P21/00C12N9/02C12N9/42C12R1/885C07G1/00
CPCC12N9/0061C12N9/0071C12N9/0059C12N1/38C12N9/2434C12N1/22C12N9/001C12N9/2437
Inventor E·A·博迪G·英格兰德
Owner DANISCO US INC
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