Composition for triggering microbiological processes in water and method of producing the same
A composition, microbial technology, applied in the field of crude oil and crude oil derived substances
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example 1
[0095] This example is an offshore situation with the aim of disintegrating crude oil derived from an oil spill.
[0096] like Figure 1-4 As shown, the only apparatus required for the practice of the present invention, such as making and using the composition of the present invention at sea, is a mother ship containing a mixture 1 of petroleum and seawater, which can easily pass through a given mixture. Pump oil 2 and seawater into a storage tank of the mother ship.
[0097] In the next step, a source of phosphorus and nitrogen 3 (including hydrophobic fibers to reduce the density and provide buoyancy of the final product), a source of oxygen and / or an oxidizing agent 4 and a batch of microbial starter 5 Add the mixture 1 of the petroleum and seawater. This liquid premix is left for a time range of 6-18 hours to provide a concentrated culture of the microorganisms.
[0098] Next, the liquid premix is transferred to a smaller vessel with a storage tank that provides for ...
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[0103] One embodiment is as follows:
[0104] The composition comprises the following components: a mixture of microbial strains, an oxygen supplier (SORA=Slow Release Oxygen Agent), a common matrix (fishmeal), an immobilization component (biopolymer) and a floating agent.
[0105] In more detail, 1 liter of a liquid solution of sodium alginate (3.0% w / v) was provided. This solution is added with 37 grams of calcium peroxide, 12 grams of fish meal, 40 grams of dry microorganisms (1x10 9 cells / gram) and 20 grams of coconut fiber, and thoroughly stirred to become a suspension, thus forming a premix. Described dry microorganism comprises following bacterial classification:
[0106] Pseudomonas stutzeri
[0107] Arthrobacter globiformis
[0108] Arthrobacter citreus
[0109] Bacillus pasteurii
[0110] Bacillus subtilis
[0111] Bacillus licheniformis
[0112] The premix was dropped into 5 liters of calcium chloride solution (6% w / v) and immobilized immediately, in the for...
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