Synthesis and catalysis process of nicotinamide containing low nicotinic acid byproduct

Abstract

The invention discloses a synthesis and catalysis process of nicotinamide containing a low nicotinic acid byproduct. The method comprises the following steps: preparing a fermentation culture medium,measuring the activity of 3-cyanopyridine hydratase in the culture medium, measuring biomass in the culture medium, carrying out a biological catalytic reaction on nicotinamide, and preserving strainsafter the catalytic reaction. According to the invention, research on production of nicotinamide by biotransformation of immobilized cells is carried out, the repeated utilization frequency of the immobilized cells is increased from 3 times to 6-7 times, and a conversion rate is 99.6%, moreover, the extraction process of nicotinamide is improved, crystallization yield is enabled to reach 88.6%, and total extraction yield reaches 88.5% ; according to the method, 3-cyanopyridine is taken as a substrate, Rhodococcus equi is taken as a catalytic strain, and the method for synthesizing nicotinamide by a microbiological process is simple in production process and stable and easy to control in catalytic process, and overcomes the problems of complex and difficult-to-control operation conditionsof a traditional batch production process based on a biological method.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a catalytic process for synthesizing nicotinamide containing low nicotinic acid by-products. Background technique [0002] Niacinamide is a water-soluble derivative of vitamin B3, which plays a role in transferring hydrogen atoms in the biological oxidation respiratory chain, can promote biological oxidation and tissue metabolism, and has a certain effect on maintaining the integrity of the skin, digestive tract and nervous system. important role. In addition, nicotinamide can be used as an additive in feed, a pharmaceutical intermediate and a nutritional enhancer in food to prevent pellagra and cardiovascular diseases. It is a very important food additive in today's society. [0003] At present, the synthesis process of nicotinamide is mainly divided into three steps: 1) cyclization to cyclize the chain organic molecule into a pyridine ring containing a methyl group; 2) me...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a catalytic process for the synthesis of nicotinamide containing low nicotinic acid by-products, using 3-cyanopyridine as the substrate and Rhodococcus equi as the catalytic strain, so that the production process is simple, the catalytic process is stable and easy to control The method for synthesizing nicotinamide by the microbial method is to solve the problem that the traditional biological batch production process proposed in the above-mentioned background technology is complicated and difficult to control.

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