63results about How to "Increase cell concentration" patented technology

Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.

The invention provides a method for producing biogas through high-solid two-phase three-stage anaerobic digestion by using perishable organic wastes, which comprises the following steps: the perishable organic wastes are hydrolyzed in a hydrolysis acidogenic reactor, and a mixture obtained after hydrolysis is acidified to generate a large number of organic acid products with low molecular weight; the bottom of the hydrolysis acidogenic reactor is communicated with the bottom of a methanogenic reactor through a circulation pump and a pipeline; the top of the methanogenic reactor is communicated with the top of the hydrolysis acidogenic reactor; and the acetic acid producing reaction is carried out in the methanogenic reactor and the methane producing reaction is continuously carried out. The invention can separate the hydrolysis acidogenic process from the acetic acid and methane producing process in the anaerobic digestion process so as to prevent organic acids generated by the perishable organic wastes from inhibiting methanogenesis. The device has simple processing procedure, low cost, easy maintenance and stable and reliable performance, is suitable for anaerobic digestion treatment of various perishable organic wastes, and has highly efficient and stable biogas production capacity of the perishable organic wastes through the anaerobic digestion.

The invention discloses a nutrient solution for arranged flowers. The nutrient solution comprises the raw materials in percentage by weight: 0.8-1.2% of saccharose, 0.7-1.4% of glucose, 0.08-0.105% of edible salt, 0.025-0.035% of aspirin, 0.1-0.14% of vitamin C, and the balance of water. Compared with the prior art, the nutrient solution has the following advantages that nutrient components required by arranged flowers are supplied to guarantee normal metabolism of the arranged flowers; the breath speed of the arranged flowers is slowed down, and the aging and withering of the arranged flowers is reduced; and because the activity of microorganism is blocked, the phenomenon of untimely rotting and decaying of the arranged flowers is avoided. By using the nutrient solution for the arranged flowers, the freshness retaining time of the arranged flowers can keep for 20-30 days, and the color is bright and the luster is lovely.

The invention relates to the microorganism agent for degrading hogwash garbage and the preparation craft, which is formed by mixing seven active germ powders in certain matching. The seven germs are as following: Bacillus subtilis E4, H11, Bacillus licheniformis A2, I1, Bacillus cereus F3, F6 and Bacillus stearothermophilus H13. The invention resolves the problems of bad degrading result and complicated preparation of the hametz due to overfull germ sorts existing in the import microorganism agent degrading the oil component in the garbage, and adopts bacillocin as the strain to obtain the germ powder through the crafts of seed culturing, fermentation culturing and sponging drying. The invention has the advantages of small sorts of germ, simple fermenting process, high concentration of germ in the germ powder and good degrading result, and can achieve minimization treatment, innocent treatment and treatment of being turned into resources, therefore can not only improve the environment sanitary of the city, but also lay a solid foundation for large-scale commercial process. The invention also has an advantage of good economic benefit.

An arrangement for taking a sample of cells from a suspicious lesion or a tumour with the so called fine needle aspiration technique, provides a good penetration of tumours, especially small and/or hard fibrous tumours, and in the meantime yielding an increased amount of cells in the sample, by applying a longitudinal movement to the needle when the needle is penetrating the tumour and by applying both a rotational and a longitudinal movement to the needle when the needle is positioned inside the tumour. The arrangement is further provided with heat generating elements in order to apply a short pulse of heat to the needle in order to lower the risk for the tumour to spread.

The invention discloses a bioreactor for high-density large-scale animal cell culture and application thereof, and belongs to the field of biological cell culture. According to the bioreactor for high-density large-scale animal cell culture, sufficient oxygen is provided in a low shear environment to meet the need of cell growth by using multi-stage microporous aeration combined with slight axial agitation or circulation, and also attenuate the dissolved oxygen gradient within the reactor. At the same time, a wire mesh demister is mounted on the top of the reactor, and external circulation spray is combined to eliminate physical damage of the cells caused by bubble breakage. The bioreactor for high-density large-scale animal cell culture has the following beneficial effects: a demisting device is arranged on the liquid layer of the reactor so that bubbles do not break on the surface of the liquid; and in addition, a gas permeable membrane is used to selectively remove dissolved carbon dioxide in the reactor to eliminate inhibition of the carbon dioxide on cell growth.

The invention relates to a growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and a using method of the growth promoting agent. The growth prompting agent is obtained as follows: adding 1 part weight of one or a mixture of sorbic acid, potassium sorbate or sodium sorbate to 1-4 parts by weight of alcohol for stirring, mixing and dissolving. The using method of the growth promoting agent is as follows: adding the growth promoting agent to a prepared photosynthetic bacteria liquid medium for stirring until the growth promoting agent is sufficiently dissolved; adding active photosynthetic bacteria strains for filling to a sealed or semi-open light transmission container for culturing at 25-32 DEG C by radiating under 1500lux-1600lux, wherein sorbate radial concentration of the growth promoting agent, which is added to the photosynthetic bacteria liquid medium, is controlled within a range of 0.005 mmol/L-1.0 mmol/L. The growth promoting agent for promoting photosynthetic bacteria strain to quickly breed is simple to use, less in usage, low in cost and capable of promoting photosynthetic bacteria strain to quickly breed. Moreover, the using method of the growth promoting agent is safe, ecological and environment-friendly and extensive in market prospect.

The invention provides a method for fermenting and producing coenzyme Q10, comprising the following steps: firstly carrying out high-density aerobic fermentation for 60 to 84 hours on agrobacterium tumefaciens by inoculum size of 5 to 15 percent; then carrying out separation on the obtained fermentation liquor and obtaining separated thallus; and finally suspending the separated thallus, then backflowing, extracting and obtaining the coenzyme Q10, wherein the fermentation culture medium comprises the following components by weight percent: 12 to 20 percent of glucose, 0.5 to 1.5 percent of ammonium sulfate, 0.1 to 0.5 percent of monopotassium phosphate, 0.01 to 0.05 percent of magnesium sulfate, 0.5 to 2.5 percent of corn steep liquor and 0.2 to 1 percent of diammonium hydrogen phosphate; and pH is 6 to 7.5 and the fermentation temperature is 28 to 31 DEG C. The method has simple technique, low thallus concentration of the fermentation liquor and low production cost and is applicable to large-scale production.

The present invention provides one method of culturing photosynthetic bacteria with ultrahigh cell density. The present invention features that in the photosynthetic bacteria culturing medium, ethanol is used as the unique organic carbon source and the ethanol concentration in the liquid culture medium is controlled in 1.0-10.0 g/L. The present invention has the advantage of making photosynthetic bacteria grow continuously to reach high cell density while remitting the suppression of raised pH value on the growth of photosynthetic bacteria.

Fine needle arrangement for taking a sample of cells from suspicious lesions by using fine needle aspiration (FNA) technique, including a tubular needle member (1), a storage compartment (2) enclosing a chamber, and a coupling (3) to which a connector may be attached. The chamber is configured with a gradually increasing cross-sectional area from the distal part of the chamber to the proximal part of the chamber, such that the chamber has no residual spaces where cell samples may be trapped, and that an efficient air streaming is achieved.

The invention discloses a method for improving efficiency of leaching chalcopyrite from thiobacillus and belongs to the technical field of bioengineering. According to the method disclosed by the invention, on the basis of an improved Starkey-chalcopyrite composite culture medium, iron ions and ferrous ions are supplemented at the initial stage of culture and elemental sulfur is added at stages inthe bio-leaching process, various biochemical reactions in the leaching environment are regulated, chemical and biological factors in the bio-leaching process are enhanced, iron metabolism is promoted while sulfur metabolism is improved, and production of sulfur passivation films and iron passivation films is reduced, so that the leaching rate is improved. The method is simple and feasible in operation, and is applicable to large-scale popularization and application of similar bio-leaching processes.

The invention discloses a biologic fresh-keeping microbial inoculum fermented with yellow serofluid generated during making bean curds and an application of the biologic fresh-keeping microbial inoculum to fresh keeping of fruits and vegetables. According to the biologic fresh-keeping microbial inoculum, fermentation liquid obtained by fermenting the yellow serofluid which is generated during making bean curds and is used as the fermentation raw material with bacillus subtilis is directly used as fresh-keeping microbial inoculum, wherein the thalli concentration in the fermentation liquid achieves 1*10<13>-2.7*10<13>CFU/ml, the gamma-polyglutamic acid concentration achieves 30-50g/L, and the antibacterial peptide yield achieves 1.2-2g/l. Compared with obtaining methods of other biologic fresh-keeping microbial inoculum, the obtaining method of the biologic fresh-keeping microbial inoculum is lower in cost and more environmental-friendly, waste resources namely the yellow serofluid generated during making bean curds can be used, so that the potential safety hazards that during natural fermentation of the yellow serofluid during processing of the bean products, sour pulp can generatepathogenic bacteria can be reduced, and besides, the gamma-polyglutamic acid content and the antibacterial material content in fermented products are high; and therefore, the biologic fresh-keeping microbial inoculum has favorable fresh-keeping effects on picked fruits and vegetables, is free from pollution and free from nuisance, and has long development prospects and industrial utilization rate.

The invention discloses a carrier attachment type cyclic culture device of a heterotrophic nitrification-aerobic denitrification bacterial agent. The carrier attachment type cyclic culture device comprises a main reactor and a pre-reactor, wherein a material supplement port and a culture medium injection port are formed at the top of each reactor, an aeration device, a sedimentation area and a discharge valve are arranged at the lower part of each reactor, the reactors are respectively connected with two solid-liquid separators through the discharge valves; and the main reactor is communicated with the solid-liquid separator of the pre-reactor through the material supplement port. The pre-reactor utilizes a residual solution after culture of the main reactor for re-culture and an unsaturated carrier is further transferred into the main reactor for further culture. The carrier attachment type cyclic culture device disclosed by the invention can fully utilize a culture medium solution, provide a pre-attachment process for the carrier, improve the utilization rate of a culture medium and the attachment efficiency of the carrier, avoid the excessive loss of the bacterial agent, improve the stability and the tolerance of the bacterial agent and realize the self-control continuous operation of the two reactors.

The invention discloses a carrier attached cyclic culture method of heterotrophic nitrification-aerobic denitrifying bacteria, wherein a light spherical filler having the diameter of 3cm is taken as a carrier, beer wastewater and various kinds of industrial wastewater are taken as a culture base fluid, and a culture device is composed of a main reactor and a pre-reactor. The pre-reactor is used for subculture by using the residual liquid left after the culture in the main reactor is finished; the unsaturated carrier is then transferred to the main reactor for further culture; the culture base liquid is utilized sufficiently and a pre-attachment process is provided for the carrier; therefore, the utilization rate of the culture medium and the attachment efficiency of the carrier are improved, and the self-control continuous operation of the two reactors is realized. The carrier attached cyclic culture method avoids too much loss of the bacteria; the bacterial can be saved more conveniently after being air-dried, high cell concentration is obtained, and the stability and endurance capacity of the bacteria are improved.

The invention discloses a method for improving soluble expression quantity of beta-cyclodextrin glucosyltransferase, and belongs to the technical field of enzyme engineering and fermentation engineering. In order to improve the yield of beta-cyclodextrin glucosyltransferase, the invention provides a strategy for improving the soluble expression of foreign protein in recombinant escherichia coli by changing the dissolved oxygen level of a fermentation culture medium and adding metal ions. An effective strategy is provided for efficient expression of the beta-cyclodextrin glucosyltransferase, the operation process is simple, the cost is low, the effect is remarkable, a certain foundation is laid for industrial production of the beta-cyclodextrin glucosyltransferase, and potential industrial application value is achieved.