Complex method for improving efficiency of chalcopyrite leaching conducted through sulfur oxidation cultures

A technology of sulfur-oxidizing bacteria and chalcopyrite, applied in the biological field, can solve problems such as low utilization efficiency, low cell culture efficiency, and slow growth rate of chemoautotrophic bacteria, so as to improve leaching efficiency, increase cell concentration, and reduce Effects of blunting threats

Active Publication Date: 2018-03-30
JIANGNAN UNIV
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Problems solved by technology

However, due to the slow growth rate of chemoautotrophic bacteria, especially due to the crystal structure of chalcopyrite, the compactness is strong, the utilization efficiency is low, and the cell culture efficiency is generally low, so it is necessary to intervene and regulate such microorganisms by means of external sources bioleaching process

Method used

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  • Complex method for improving efficiency of chalcopyrite leaching conducted through sulfur oxidation cultures
  • Complex method for improving efficiency of chalcopyrite leaching conducted through sulfur oxidation cultures
  • Complex method for improving efficiency of chalcopyrite leaching conducted through sulfur oxidation cultures

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1 High-density cultivation of Thiobacillus thiooxidans and cell collection without sulfur slag and slag

[0022] Starkey complex medium consists of three parts, the first part contains (NH 4 ) 2 SO 4 0.3g, KH 2 PO 4 3.0g, MgSO 4 ·7H 2 O 0.5g, CaCl 2 0.25g, Fe 2 (SO 4 ) 3 ·7H 2 O 0.018g, H 2 O 1000 mL; the second part: elemental sulfur 5g / L; the third part 0.5% (w / v) chalcopyrite-deficient; the first and second parts are sterilized and mixed to adjust the pH to 2.0. The ore samples were taken from the Tonglingshan mining area in Anhui Province. After preliminary crushing and filtration with steel sieves, the ore particle size is about <48μm. The first part is sterilized at 121°C for 20 minutes by autoclaving. Then the two parts were mixed and shaken, and the pH of the solution was adjusted to 2.0 with 2 mol / L hydrochloric acid. Thiobacillus thiooxidans ZJJN was inoculated into Starkey complex basal medium for culture. When the reduced sulfur in ...

Embodiment 2

[0026] Example 2 Appropriate supplementation of iron ions and elemental sulfur in the early stage of leaching shortens the lag period

[0027] The chalcopyrite compound culture medium adopts Starkey basal medium (formula (NH 4 ) 2 SO 4 0.3g, KH 2 PO 4 3.0g, MgSO 4 ·7H 2 O 0.5g, CaCl 2 0.25g, Fe 2 (SO 4 ) 3 ·7H 2 O 0.018g, H 2 (2 1000mL), the control chalcopyrite slurry concentration is 2.0%; 30 ℃ of temperature, shaker speed 170rpm, control initial cell by inoculation about 5.0 * 10 7 individual / mL. Add 2.0g / L iron ion and 2.0g / L elemental sulfur during the inoculation process of the leaching system (the inoculum size is 5.0×10 7 / mL), the key parameters and leaching results of the bacterial growth in the leaching process are shown in Table 2. Maximum specific growth rate changed from 0.40d -1 Improve to 0.52d -1 , and the time required to reach the maximum specific growth rate is reduced from 11.5d to 9.5d. Add 2.0g / L Fe 3+and 1.0g / L elemental sulfur obta...

Embodiment 3

[0030] Example 3 Constant pH Coupled Pulse Supplementation of Sulfur Oxidizing Bacteria Cells to Enhance Iron / Sulphur Metabolism in the Late Stage of Leaching

[0031] Since the introduction of some iron ions in the early stage enhances iron metabolism, jarosite will be produced in the later stage, covering the mineral surface and making leaching impossible, which is the key factor for the low bioleaching rate of chalcopyrite. 15d begins after leaching begins (adding 2.0g / LFe of other steps and embodiment 2 3+ Consistent with the 1.0g / L elemental sulfur system), 6mol / L hydrochloric acid is used to adjust the pH of the leachate to 1.0 every day to maintain a relatively stable acidic environment and reduce the generation of jarosite. At the same time, add 5.0×10 every three days 7 Each / mL Thiobacillus thiooxidans cells can continuously strengthen the biological effect of the leaching process, and the hydrogen ions produced while enhancing sulfur metabolism can also resist the p...

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Abstract

The invention discloses a complex method for improving efficiency of chalcopyrite leaching conducted through sulfur oxidation cultures and belongs to the technical field of biology. The method comprises the steps that on the basis of a Starkey-chalcopyrite composite culture medium, an external-source energy substrate, namely elemental sulfur, is supplemented in a pulsed manner at the middle and later culture stages, and high-density culture of thiobacillus thiooxidans is achieved; a proper amount of elemental sulfur and iron ions are added while cell inoculation is conducted, start of a leaching biochemical reaction cycle is accelerated, the lag phase is shortened, and the inoculum size is increased; and at the later leaching stage, the low constant pH is maintained, jarosite stress is inhibited, sulfur oxidation culture cells are added as supplementation, iron metabolism is promoted while sulfur metabolism is improved, and furthermore, the leaching microenvironment is improved. By means of the method, the sulfur oxidation cultures can be cultured more efficiently, the leaching lag phase is shortened, and active biochemical reaction state of the leaching microenvironment is maintained better, and thus the leaching rate is increased; and in addition, the method is easy to operate and implement and suitable for being applied and popularized in similar bioleaching processes on a large scale.

Description

technical field [0001] The invention relates to a composite method for improving the chalcopyrite leaching efficiency of sulfur-oxidizing bacteria, and belongs to the field of biotechnology. Background technique [0002] Due to the lack of reasonable and effective mining methods and the high cost of traditional smelting, most of the low-grade ores are discarded and wasted, which in turn leads to heavy metal pollution in the environment. With the increase of high-grade rich ore mining and the decline of reserves, it is becoming more and more urgent to use high-grade ore more effectively. Chalcopyrite is the copper resource with the most abundant reserves, but its grade is usually low and its structure is complex, and conventional high-temperature smelting is difficult to realize its economic value, which makes this inevitability even more important in the mining of copper resources. Compared with traditional thermal power smelting, bioleaching has the advantages of simple op...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C22B3/18C22B15/00C12N1/20C12R1/01
CPCC12N1/20C22B3/18C22B15/0065Y02P10/20
Inventor 冯守帅杨海麟黄壮壮吕妍骅宣紫荆张蕾蕾
Owner JIANGNAN UNIV
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