A kind of environment-friendly antifreeze agent for fruit trees and preparation method thereof
An environmentally friendly, antifreeze technology, applied in the fields of botanical equipment and methods, chemicals for biological control, applications, etc., can solve the problems of strong inhibition of plant germination, reduced plant growth and development, limited antifreeze effect, etc. Action time, prolonged effective antifreeze period, lasting effect of antifreeze effect
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Embodiment 1
[0038] 1. Preparation of nano liposome-like solution of plant cell resurrection agent:
[0039] 1) After dissolving 0.8 g of soy lecithin and 0.06 g of β-sitosterol with chloroform ultrasonically, they are de-dissolved under reduced pressure at 20-30℃ to obtain a smooth phospholipid film;
[0040] 2) Add 20 mg of seaweed polysaccharides, 20 mg of soybean peptides, and 10 mg of surfactant PVP-K15 to the phospholipid film, then add 20 ml of pH 7.0 phosphate buffer, and diffuse under ultrasonic conditions in a water bath. After ultrasonic diffusion, place it in a cell ultrafine pulverizer. Crush (working 2 s / gap 2 s, power 350 W, 20 min), then centrifuge the resulting product (5°C, 6000 r / min, 10 min) to remove the lower layer of impurities, and finally filter with 0.22 microporous membrane to obtain The filtrate is the nano liposome-like solution of plant cell resurrection agent.
[0041] 2. Preparation of the nano-capsule dispersion of calcium cyclamate:
[0042] 1) 1.0g of calcium he...
Embodiment 2
[0054] The antifreeze performance test was carried out on the environment-friendly antifreeze emulsions for fruit trees prepared in Example 1 and Comparative Examples 1 and 2. The red Fuji apple tree (tree age 8 years) in Zhaoyuan, Yantai was used as the test phase. Mid-to-late month) Weather forecast There will be frost forecast in the early morning of April 26 (only on the same day). The environment-friendly antifreeze emulsion for fruit trees prepared in Example 1, Comparative Examples 1 and 2 (15-20kg per 20g with water) is in April Spray the whole apple tree canopy around noon on the 25th (one antifreeze is used for every 100 trees); the lowest temperature from the night of April 25th to the morning of the 26th is -3.2℃. At noon on April 28th, every tree canopy and middle A total of 90 unfolded petals adjacent to the bottom, all flowers whose floral organs turn light brown or brown are injured flowers (if among 90, the injured flower ≤ 1 is regarded as unfrozen, 2 ≤ the inj...
Embodiment 3
[0060] The antifreeze performance test of the environmentally friendly antifreeze agent emulsion for fruit trees prepared in Example 1 and Comparative Examples 1 and 2 was carried out with pear trees (6 years old) in Laiyang, Yantai as the test phase. During the pear blossom period, the weather forecast 2015 There was a cooling forecast in the early morning of March 09, 2010. The environment-friendly antifreeze emulsion for fruit trees prepared in Example 1, Comparative Examples 1 and 2 (15-20 kg per 20g of water) was used to perform the whole pear tree around noon on March 03 Spraying (one type of antifreeze is used for every 100 trees); the lowest temperature from the night of March 08 to the morning of March 09 is -3.6°C, at noon on March 10, a total of 90 pear flowers adjacent to the top, middle and bottom of each tree , The flowers whose floral organs turn black or brown are injured flowers (if among 90 flowers, the injured flower ≤ 1 is regarded as unfrozen; 2 ≤ injured fl...
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