Cyclic beta-1,2-glucan fermenting method capable of inhibiting chromatogenesis

A technology of dextran and production method, which is applied in the field of intelligent light industry process control, and can solve problems such as the inability to increase the cell concentration and the deepening of the color of the fermentation broth

Active Publication Date: 2018-02-02
JIANGNAN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

This is mainly because the strains producing cyclic β-1,2-glucan mainly use glutamic acid as the nitrogen source. Generally speaking, increasing the nitrogen source can increase the cell concentration, but when increasing the concentration of the nitrogen source glutamic acid, Pigmentation will occur, darkening the broth and causing the cell concentration to fail to increase
The improvement of cell biomass is closely related to the yield of cyclic β-1,2-glucan; at the same time, the formation of pigment will increase the cost of removing pigment in the subsequent purification process of cyclic β-1,2-glucan

Method used

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  • Cyclic beta-1,2-glucan fermenting method capable of inhibiting chromatogenesis
  • Cyclic beta-1,2-glucan fermenting method capable of inhibiting chromatogenesis
  • Cyclic beta-1,2-glucan fermenting method capable of inhibiting chromatogenesis

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Embodiment 1

[0030] Seed medium (g / L): peptone 10, yeast extract 2, MgSO 4 ·7H 2 O 1, adjust the pH to 7.0-7.2, and sterilize at 121°C for 20 minutes.

[0031] The fermentation medium is (g / L) mannitol 10.0, glutamic acid 5.0, NaCl 0.2, K 2 HPO 4 1.0,MgSO 4 ·7H 2 O0.2, CaCl 2 2H 2 O 0.04, FeCl 3 ·6H 2 O 0.0025, MnCl 2 4H 2 O 0.001,Na 2 MO 4 ·7H 2 O 0.00001, ZnSO 4 ·7H 2 O 0.00001, CuSO 4 ·5H 2 O 0.00001,H 3 BO 3 0.00001,CoCl 2 ·6H 2 O 0.00001, biotin 0.00001, vitamin B10.00001, pH 7.0–7.2.

[0032]Inoculate Rhizobium radiobacter NBRC13259 into the seed medium, cultivate at 30°C for 20 hours, inoculate the seed solution at a ratio of 10%, and the number of bacteria in the seed solution is 1.2×10 10 CFU / mL.

[0033] The fermentation process is based on the PID system to control the pH, and at the same time, the fermentation process of Rhizobium radiobacter NBRC13259 to produce cyclic β-1,2-glucan by adding glutamic acid to supplement the nitrogen source with timed puls...

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Abstract

The invention discloses a cyclic beta-1,2-glucan fermenting method capable of inhibiting chromatogenesis and belongs to the field of intelligent light industry process control. The cyclic beta-1,2-glucan fermenting method disclosed by the invention successfully inhibits chromatogenesis in a Rhizobium radiobacter NBRC 13259 fermenting process, improves a cell concentration to 8.0g / L, solves the problems of difficulty in controlling chromatogenesis and difficulty in improving the cell concentration in the Rhizobium radiobacter NBRC 13259 fermenting process and establishes foundation for improving a cyclic beta-1,2-glucan concentration in a unit volume.

Description

technical field [0001] The invention relates to a cyclic β-1,2-glucan fermentation method for inhibiting pigment formation, and belongs to the field of intelligent light industry process control. Background technique [0002] Cyclic β-1,2-glucan is a cyclic polysaccharide with glucose as a monomer, linked by β-1,2 glycosidic bonds, with a degree of polymerization between 17 and 25 and strong water solubility. It plays a key role in the formation of nodules, the invasion of the body's cells by Brucella bacteria, and their intracellular escape from the body's natural immune system. Obtaining enough cyclic β-1,2-glucan is the basis for analyzing its function, but there is no effective method and way to obtain a large amount of β-1,2-glucan. [0003] Through the research on Rhizobium meliloti MTCC 3402 to produce cyclic β-1,2-glucan by fermentation method, the highest biomass obtained is only 1.8g / L. This is mainly because the strains producing cyclic β-1,2-glucan mainly use g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08C12R1/41
CPCC12P19/08
Inventor 詹晓北曲娟娟张洪涛朱莉周献超
Owner JIANGNAN UNIV
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